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Online Microextraction Coupled with HPLC-ABTS for Rapid Analysis of Antioxidants from the Root of Polygonum bistorta

The root of Polygonum bistorta (PB) is a traditional Chinese medicinal plant material widely used in China. It has been commonly used for the treatment of hemostasis, detumescence, diarrhea, snake bite, and acute gastroenteritis. However, the research on the antioxidant properties and bioactive comp...

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Autores principales: Yang, Wei-Qi, Qian, Zheng-Ming, Wu, Meng-Qi, Gao, Jian-Li, Huang, Qi, Zou, Yuan-Sheng, Tang, Dan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9873428/
https://www.ncbi.nlm.nih.gov/pubmed/36704212
http://dx.doi.org/10.1155/2023/7496848
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author Yang, Wei-Qi
Qian, Zheng-Ming
Wu, Meng-Qi
Gao, Jian-Li
Huang, Qi
Zou, Yuan-Sheng
Tang, Dan
author_facet Yang, Wei-Qi
Qian, Zheng-Ming
Wu, Meng-Qi
Gao, Jian-Li
Huang, Qi
Zou, Yuan-Sheng
Tang, Dan
author_sort Yang, Wei-Qi
collection PubMed
description The root of Polygonum bistorta (PB) is a traditional Chinese medicinal plant material widely used in China. It has been commonly used for the treatment of hemostasis, detumescence, diarrhea, snake bite, and acute gastroenteritis. However, the research on the antioxidant properties and bioactive compounds from PB is inadequate. In the current research, an online microextraction (OLME) coupled with a high-performance liquid chromatography coupled with the 2,2-nitrogen-di (3-ethyl-benzothiazole-6-sulfonic acid) diammonium salt antioxidant assay (HPLC-ABTS) system for rapid analysis of antioxidants from PB was proposed. The PB sample (0.17 mg) was online extracted by mobile phase (acetonitrile and 0.2% acetic acid); a Poroshell 120 SB-Aq column was used for separation; then, an online ABTS assay system was used for screening the antioxidants. Finally, ten components were found in PB, and among them, eight components possessed antioxidant activities. Furthermore, five components (gallic acid, neochlorogenic acid, caffeic acid, chlorogenic acid, and an unknown compound) were proved as major antioxidants when compared with rutin as an antioxidant marker. The results showed that the developed OLME-HPLC-ABTS system was a simple, rapid, green, and efficient instrument for the screening of antioxidants from PB, which provides a powerful tool for the discovery of natural antioxidants in Chinese medicines.
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spelling pubmed-98734282023-01-25 Online Microextraction Coupled with HPLC-ABTS for Rapid Analysis of Antioxidants from the Root of Polygonum bistorta Yang, Wei-Qi Qian, Zheng-Ming Wu, Meng-Qi Gao, Jian-Li Huang, Qi Zou, Yuan-Sheng Tang, Dan Evid Based Complement Alternat Med Research Article The root of Polygonum bistorta (PB) is a traditional Chinese medicinal plant material widely used in China. It has been commonly used for the treatment of hemostasis, detumescence, diarrhea, snake bite, and acute gastroenteritis. However, the research on the antioxidant properties and bioactive compounds from PB is inadequate. In the current research, an online microextraction (OLME) coupled with a high-performance liquid chromatography coupled with the 2,2-nitrogen-di (3-ethyl-benzothiazole-6-sulfonic acid) diammonium salt antioxidant assay (HPLC-ABTS) system for rapid analysis of antioxidants from PB was proposed. The PB sample (0.17 mg) was online extracted by mobile phase (acetonitrile and 0.2% acetic acid); a Poroshell 120 SB-Aq column was used for separation; then, an online ABTS assay system was used for screening the antioxidants. Finally, ten components were found in PB, and among them, eight components possessed antioxidant activities. Furthermore, five components (gallic acid, neochlorogenic acid, caffeic acid, chlorogenic acid, and an unknown compound) were proved as major antioxidants when compared with rutin as an antioxidant marker. The results showed that the developed OLME-HPLC-ABTS system was a simple, rapid, green, and efficient instrument for the screening of antioxidants from PB, which provides a powerful tool for the discovery of natural antioxidants in Chinese medicines. Hindawi 2023-01-17 /pmc/articles/PMC9873428/ /pubmed/36704212 http://dx.doi.org/10.1155/2023/7496848 Text en Copyright © 2023 Wei-Qi Yang et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Yang, Wei-Qi
Qian, Zheng-Ming
Wu, Meng-Qi
Gao, Jian-Li
Huang, Qi
Zou, Yuan-Sheng
Tang, Dan
Online Microextraction Coupled with HPLC-ABTS for Rapid Analysis of Antioxidants from the Root of Polygonum bistorta
title Online Microextraction Coupled with HPLC-ABTS for Rapid Analysis of Antioxidants from the Root of Polygonum bistorta
title_full Online Microextraction Coupled with HPLC-ABTS for Rapid Analysis of Antioxidants from the Root of Polygonum bistorta
title_fullStr Online Microextraction Coupled with HPLC-ABTS for Rapid Analysis of Antioxidants from the Root of Polygonum bistorta
title_full_unstemmed Online Microextraction Coupled with HPLC-ABTS for Rapid Analysis of Antioxidants from the Root of Polygonum bistorta
title_short Online Microextraction Coupled with HPLC-ABTS for Rapid Analysis of Antioxidants from the Root of Polygonum bistorta
title_sort online microextraction coupled with hplc-abts for rapid analysis of antioxidants from the root of polygonum bistorta
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9873428/
https://www.ncbi.nlm.nih.gov/pubmed/36704212
http://dx.doi.org/10.1155/2023/7496848
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