Nano-probe for determination of phenobarbital of green synthesized fluorescent carbon dots using Scrophularia striata

In this study, using a thirsty plant extract and a simple hydrothermal method, a nano-probe was introduced to detect the phenobarbital based on fluorescence. Functional groups, particle size, surface morphology, and types of elements were identified using analysis such as FTIR, TEM, SEM, EDX, respectively. The excitation at 355 nm and emission intensity at 446 nm for nano-probe, the nano-probe shows that various parameters such as pH, temperature, and time were investigated for optimization conditions. After optimizing the factors affecting the sensor’s response, a linear range between 0 and 750 µM with a detection limit of 5 µM was obtained. Then, the effect of interfering with other materials was investigated and finally, the ability of this sensor to measure the phenobarbital in real samples has been studied.