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Cold tolerance strategy and cryoprotectants of Megabruchidius dorsalis in different temperature and time stresses

The honey locusts (genus Gleditsia) are a genus of high-value trees in Asia. Seed beetle, Megabruchidius dorsalis (Fåhraeus) (Col.: Chrysomelidae: Bruchinae), is a Gleditsia oligophagous pest that causes severe yield reduction. To understand the cold tolerance of M. dorsalis adults, this study inves...

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Autores principales: Chen, Si-Yu, Zhao, Ru-Na, Li, You, Li, He-Ping, Xie, Ming-Hui, Liu, Jian-Feng, Yang, Mao-Fa, Wu, Cheng-Xu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9873968/
https://www.ncbi.nlm.nih.gov/pubmed/36714316
http://dx.doi.org/10.3389/fphys.2022.1118955
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author Chen, Si-Yu
Zhao, Ru-Na
Li, You
Li, He-Ping
Xie, Ming-Hui
Liu, Jian-Feng
Yang, Mao-Fa
Wu, Cheng-Xu
author_facet Chen, Si-Yu
Zhao, Ru-Na
Li, You
Li, He-Ping
Xie, Ming-Hui
Liu, Jian-Feng
Yang, Mao-Fa
Wu, Cheng-Xu
author_sort Chen, Si-Yu
collection PubMed
description The honey locusts (genus Gleditsia) are a genus of high-value trees in Asia. Seed beetle, Megabruchidius dorsalis (Fåhraeus) (Col.: Chrysomelidae: Bruchinae), is a Gleditsia oligophagous pest that causes severe yield reduction. To understand the cold tolerance of M. dorsalis adults, this study investigated its cold tolerance strategy and the influence of low temperatures on its physiology and biochemistry. The low-temperature treatments were divided into three groups: long-term temperature acclimation (Group 1; 15°C, or 20°C, or 25°C, or 28°C [control check, CK] for 10 days), short-term low-temperature exposure (Group 2; 0°C or 4°C for 2 h), and long-term low-temperature induction (Group 3; 0°C or 4°C for 1, 3, or 5 d). The supercooling point (SCP; temperature at which spontaneous nucleation and ice lattice growth begin), freezing point (FP; temperature at which insect fluids freeze), low lethal temperature (LLT; temperature at which all individuals are killed), water, lipid, glycerol, and total sugars contents were measured under different temperature stresses. The results showed that M. dorsalis adults were a freeze-avoidant species. The SCP and LLT at 28°C were −10.62°C and −19.48°C, respectively. The SCP and FP of long-term temperature acclimation (15°C, or 20°C, or 25°C) were significantly lower than that of the control group (28°C). The water content of the long-term low temperature induction (0°C) group was significantly lower than that of the control group. The lipid and glycerol content in the acclimated group at 20°C and 25°C were significantly higher than in the control group. M. dorsalis adults may maintain their biofluids in a supercooled state via cryoprotectant accumulation and cryoprotective dehydration to prevent ice nucleation. This study provides a theoretical basis for future research on overwintering and potential distribution and related prediction of M. dorsalis adults.
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spelling pubmed-98739682023-01-26 Cold tolerance strategy and cryoprotectants of Megabruchidius dorsalis in different temperature and time stresses Chen, Si-Yu Zhao, Ru-Na Li, You Li, He-Ping Xie, Ming-Hui Liu, Jian-Feng Yang, Mao-Fa Wu, Cheng-Xu Front Physiol Physiology The honey locusts (genus Gleditsia) are a genus of high-value trees in Asia. Seed beetle, Megabruchidius dorsalis (Fåhraeus) (Col.: Chrysomelidae: Bruchinae), is a Gleditsia oligophagous pest that causes severe yield reduction. To understand the cold tolerance of M. dorsalis adults, this study investigated its cold tolerance strategy and the influence of low temperatures on its physiology and biochemistry. The low-temperature treatments were divided into three groups: long-term temperature acclimation (Group 1; 15°C, or 20°C, or 25°C, or 28°C [control check, CK] for 10 days), short-term low-temperature exposure (Group 2; 0°C or 4°C for 2 h), and long-term low-temperature induction (Group 3; 0°C or 4°C for 1, 3, or 5 d). The supercooling point (SCP; temperature at which spontaneous nucleation and ice lattice growth begin), freezing point (FP; temperature at which insect fluids freeze), low lethal temperature (LLT; temperature at which all individuals are killed), water, lipid, glycerol, and total sugars contents were measured under different temperature stresses. The results showed that M. dorsalis adults were a freeze-avoidant species. The SCP and LLT at 28°C were −10.62°C and −19.48°C, respectively. The SCP and FP of long-term temperature acclimation (15°C, or 20°C, or 25°C) were significantly lower than that of the control group (28°C). The water content of the long-term low temperature induction (0°C) group was significantly lower than that of the control group. The lipid and glycerol content in the acclimated group at 20°C and 25°C were significantly higher than in the control group. M. dorsalis adults may maintain their biofluids in a supercooled state via cryoprotectant accumulation and cryoprotective dehydration to prevent ice nucleation. This study provides a theoretical basis for future research on overwintering and potential distribution and related prediction of M. dorsalis adults. Frontiers Media S.A. 2023-01-11 /pmc/articles/PMC9873968/ /pubmed/36714316 http://dx.doi.org/10.3389/fphys.2022.1118955 Text en Copyright © 2023 Chen, Zhao, Li, Li, Xie, Liu, Yang and Wu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Chen, Si-Yu
Zhao, Ru-Na
Li, You
Li, He-Ping
Xie, Ming-Hui
Liu, Jian-Feng
Yang, Mao-Fa
Wu, Cheng-Xu
Cold tolerance strategy and cryoprotectants of Megabruchidius dorsalis in different temperature and time stresses
title Cold tolerance strategy and cryoprotectants of Megabruchidius dorsalis in different temperature and time stresses
title_full Cold tolerance strategy and cryoprotectants of Megabruchidius dorsalis in different temperature and time stresses
title_fullStr Cold tolerance strategy and cryoprotectants of Megabruchidius dorsalis in different temperature and time stresses
title_full_unstemmed Cold tolerance strategy and cryoprotectants of Megabruchidius dorsalis in different temperature and time stresses
title_short Cold tolerance strategy and cryoprotectants of Megabruchidius dorsalis in different temperature and time stresses
title_sort cold tolerance strategy and cryoprotectants of megabruchidius dorsalis in different temperature and time stresses
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9873968/
https://www.ncbi.nlm.nih.gov/pubmed/36714316
http://dx.doi.org/10.3389/fphys.2022.1118955
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