Characteristics of exopolysaccharides produced by isolates from natural bioflocculant of Ruditapes philippinarum conglutination mud

Three novel types of exopolysaccharides (EPS) EPS-S8, EPS-S5, and EPS-F10 were extracted and purified from bacterial isolates Bacillus sp. GHS8, Pseudoalteromonas sp. GHS5 and Psychrobacter sp. GHF10, which were originated from natural bioflocculant of Ruditapes philippinarum conglutination mud (RPM...

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Autores principales: Feng, Lijuan, Qian, Tingting, Yang, Guangfeng, Mu, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9874098/
https://www.ncbi.nlm.nih.gov/pubmed/36713164
http://dx.doi.org/10.3389/fmicb.2022.1068922
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author Feng, Lijuan
Qian, Tingting
Yang, Guangfeng
Mu, Jun
author_facet Feng, Lijuan
Qian, Tingting
Yang, Guangfeng
Mu, Jun
author_sort Feng, Lijuan
collection PubMed
description Three novel types of exopolysaccharides (EPS) EPS-S8, EPS-S5, and EPS-F10 were extracted and purified from bacterial isolates Bacillus sp. GHS8, Pseudoalteromonas sp. GHS5 and Psychrobacter sp. GHF10, which were originated from natural bioflocculant of Ruditapes philippinarum conglutination mud (RPM), respectively. The EPS had similar function groups C-H, N-H, C-O, and C = O. The EPS were composed of different monosaccharides (EPS-F10, Man: GlcN: GlcUA: GalUA = 1:0.66:5.75:0.51; EPS-S5, Man: Gal: GlcN: Rib = 1: 0.50: 2.94: 0.26; EPS-S8, Man: Gal: GlcN = 1:1.54:7.69). The molecular weights (Mw) of EPS were ordered as 51.4 kDa (EPS-S5) > 9.15 kDa (EPS-S8) > 4.41 kDa (EPS-F10). Three types of EPS all showed higher peak flocculation activities than the reported crude EPS from the RPM. Besides, the EPS also exhibited efficient decoloration and antioxidation activities, especially for EPS-S8, which might be due to the low Mw and specific monosaccharide composition.
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spelling pubmed-98740982023-01-26 Characteristics of exopolysaccharides produced by isolates from natural bioflocculant of Ruditapes philippinarum conglutination mud Feng, Lijuan Qian, Tingting Yang, Guangfeng Mu, Jun Front Microbiol Microbiology Three novel types of exopolysaccharides (EPS) EPS-S8, EPS-S5, and EPS-F10 were extracted and purified from bacterial isolates Bacillus sp. GHS8, Pseudoalteromonas sp. GHS5 and Psychrobacter sp. GHF10, which were originated from natural bioflocculant of Ruditapes philippinarum conglutination mud (RPM), respectively. The EPS had similar function groups C-H, N-H, C-O, and C = O. The EPS were composed of different monosaccharides (EPS-F10, Man: GlcN: GlcUA: GalUA = 1:0.66:5.75:0.51; EPS-S5, Man: Gal: GlcN: Rib = 1: 0.50: 2.94: 0.26; EPS-S8, Man: Gal: GlcN = 1:1.54:7.69). The molecular weights (Mw) of EPS were ordered as 51.4 kDa (EPS-S5) > 9.15 kDa (EPS-S8) > 4.41 kDa (EPS-F10). Three types of EPS all showed higher peak flocculation activities than the reported crude EPS from the RPM. Besides, the EPS also exhibited efficient decoloration and antioxidation activities, especially for EPS-S8, which might be due to the low Mw and specific monosaccharide composition. Frontiers Media S.A. 2023-01-11 /pmc/articles/PMC9874098/ /pubmed/36713164 http://dx.doi.org/10.3389/fmicb.2022.1068922 Text en Copyright © 2023 Feng, Qian, Yang and Mu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Feng, Lijuan
Qian, Tingting
Yang, Guangfeng
Mu, Jun
Characteristics of exopolysaccharides produced by isolates from natural bioflocculant of Ruditapes philippinarum conglutination mud
title Characteristics of exopolysaccharides produced by isolates from natural bioflocculant of Ruditapes philippinarum conglutination mud
title_full Characteristics of exopolysaccharides produced by isolates from natural bioflocculant of Ruditapes philippinarum conglutination mud
title_fullStr Characteristics of exopolysaccharides produced by isolates from natural bioflocculant of Ruditapes philippinarum conglutination mud
title_full_unstemmed Characteristics of exopolysaccharides produced by isolates from natural bioflocculant of Ruditapes philippinarum conglutination mud
title_short Characteristics of exopolysaccharides produced by isolates from natural bioflocculant of Ruditapes philippinarum conglutination mud
title_sort characteristics of exopolysaccharides produced by isolates from natural bioflocculant of ruditapes philippinarum conglutination mud
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9874098/
https://www.ncbi.nlm.nih.gov/pubmed/36713164
http://dx.doi.org/10.3389/fmicb.2022.1068922
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