Cargando…
Portulaca oleracea L. organic acid extract inhibits persistent methicillin-resistant Staphylococcus aureus in vitro and in vivo
Staphylococcus aureus continues to be one of the most important pathogens capable of causing a wide range of infections in different sites of the body in humans and livestock. With the emergence of methicillin-resistant strains and the introduction of strict laws on antibiotic usage in animals, anti...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9874160/ https://www.ncbi.nlm.nih.gov/pubmed/36713183 http://dx.doi.org/10.3389/fmicb.2022.1076154 |
_version_ | 1784877744612442112 |
---|---|
author | Liu, Gengsong Liu, Aijing Yang, Cheng Zhou, Congcong Zhou, Qiaoyan Li, Haizhu Yang, Hongchun Mo, Jiahao Zhang, Zhidan Li, Gonghe Si, Hongbin Ou, Changbo |
author_facet | Liu, Gengsong Liu, Aijing Yang, Cheng Zhou, Congcong Zhou, Qiaoyan Li, Haizhu Yang, Hongchun Mo, Jiahao Zhang, Zhidan Li, Gonghe Si, Hongbin Ou, Changbo |
author_sort | Liu, Gengsong |
collection | PubMed |
description | Staphylococcus aureus continues to be one of the most important pathogens capable of causing a wide range of infections in different sites of the body in humans and livestock. With the emergence of methicillin-resistant strains and the introduction of strict laws on antibiotic usage in animals, antibiotic replacement therapy has become increasingly popular. Previous studies have shown that Portulaca oleracea L. extract exerts a certain degree of bacteriostatic effect, although the active ingredients are unknown. In the present study, the antibacterial activity of the organic acid of P. oleracea (OAPO) against S. aureus was examined using a series of experiments, including the minimum inhibitory concentration, growth curve, and bacteriostasis curve. In vitro antibacterial mechanisms were evaluated based on the integrity and permeability of the cell wall and membrane, scanning electron microscopy, and soluble protein content. A mouse skin wound recovery model was used to verify the antibacterial effects of OAPO on S. aureus in vivo. The results showed that OAPO not only improved skin wound recovery but also decreased the bacterial load in skin wounds. Moreover, the number of inflammatory cells and cytokines decreased in the OAPO-treated groups. In summary, this study reports a botanical extract that can inhibit S. aureus in vitro and in vivo, indicating the potential use of OAPO to prevent and control S. aureus infection in the near future. |
format | Online Article Text |
id | pubmed-9874160 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-98741602023-01-26 Portulaca oleracea L. organic acid extract inhibits persistent methicillin-resistant Staphylococcus aureus in vitro and in vivo Liu, Gengsong Liu, Aijing Yang, Cheng Zhou, Congcong Zhou, Qiaoyan Li, Haizhu Yang, Hongchun Mo, Jiahao Zhang, Zhidan Li, Gonghe Si, Hongbin Ou, Changbo Front Microbiol Microbiology Staphylococcus aureus continues to be one of the most important pathogens capable of causing a wide range of infections in different sites of the body in humans and livestock. With the emergence of methicillin-resistant strains and the introduction of strict laws on antibiotic usage in animals, antibiotic replacement therapy has become increasingly popular. Previous studies have shown that Portulaca oleracea L. extract exerts a certain degree of bacteriostatic effect, although the active ingredients are unknown. In the present study, the antibacterial activity of the organic acid of P. oleracea (OAPO) against S. aureus was examined using a series of experiments, including the minimum inhibitory concentration, growth curve, and bacteriostasis curve. In vitro antibacterial mechanisms were evaluated based on the integrity and permeability of the cell wall and membrane, scanning electron microscopy, and soluble protein content. A mouse skin wound recovery model was used to verify the antibacterial effects of OAPO on S. aureus in vivo. The results showed that OAPO not only improved skin wound recovery but also decreased the bacterial load in skin wounds. Moreover, the number of inflammatory cells and cytokines decreased in the OAPO-treated groups. In summary, this study reports a botanical extract that can inhibit S. aureus in vitro and in vivo, indicating the potential use of OAPO to prevent and control S. aureus infection in the near future. Frontiers Media S.A. 2023-01-11 /pmc/articles/PMC9874160/ /pubmed/36713183 http://dx.doi.org/10.3389/fmicb.2022.1076154 Text en Copyright © 2023 Liu, Liu, Yang, Zhou, Zhou, Li, Yang, Mo, Zhang, Li, Si and Ou. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Liu, Gengsong Liu, Aijing Yang, Cheng Zhou, Congcong Zhou, Qiaoyan Li, Haizhu Yang, Hongchun Mo, Jiahao Zhang, Zhidan Li, Gonghe Si, Hongbin Ou, Changbo Portulaca oleracea L. organic acid extract inhibits persistent methicillin-resistant Staphylococcus aureus in vitro and in vivo |
title | Portulaca oleracea L. organic acid extract inhibits persistent methicillin-resistant Staphylococcus aureus in vitro and in vivo |
title_full | Portulaca oleracea L. organic acid extract inhibits persistent methicillin-resistant Staphylococcus aureus in vitro and in vivo |
title_fullStr | Portulaca oleracea L. organic acid extract inhibits persistent methicillin-resistant Staphylococcus aureus in vitro and in vivo |
title_full_unstemmed | Portulaca oleracea L. organic acid extract inhibits persistent methicillin-resistant Staphylococcus aureus in vitro and in vivo |
title_short | Portulaca oleracea L. organic acid extract inhibits persistent methicillin-resistant Staphylococcus aureus in vitro and in vivo |
title_sort | portulaca oleracea l. organic acid extract inhibits persistent methicillin-resistant staphylococcus aureus in vitro and in vivo |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9874160/ https://www.ncbi.nlm.nih.gov/pubmed/36713183 http://dx.doi.org/10.3389/fmicb.2022.1076154 |
work_keys_str_mv | AT liugengsong portulacaoleracealorganicacidextractinhibitspersistentmethicillinresistantstaphylococcusaureusinvitroandinvivo AT liuaijing portulacaoleracealorganicacidextractinhibitspersistentmethicillinresistantstaphylococcusaureusinvitroandinvivo AT yangcheng portulacaoleracealorganicacidextractinhibitspersistentmethicillinresistantstaphylococcusaureusinvitroandinvivo AT zhoucongcong portulacaoleracealorganicacidextractinhibitspersistentmethicillinresistantstaphylococcusaureusinvitroandinvivo AT zhouqiaoyan portulacaoleracealorganicacidextractinhibitspersistentmethicillinresistantstaphylococcusaureusinvitroandinvivo AT lihaizhu portulacaoleracealorganicacidextractinhibitspersistentmethicillinresistantstaphylococcusaureusinvitroandinvivo AT yanghongchun portulacaoleracealorganicacidextractinhibitspersistentmethicillinresistantstaphylococcusaureusinvitroandinvivo AT mojiahao portulacaoleracealorganicacidextractinhibitspersistentmethicillinresistantstaphylococcusaureusinvitroandinvivo AT zhangzhidan portulacaoleracealorganicacidextractinhibitspersistentmethicillinresistantstaphylococcusaureusinvitroandinvivo AT ligonghe portulacaoleracealorganicacidextractinhibitspersistentmethicillinresistantstaphylococcusaureusinvitroandinvivo AT sihongbin portulacaoleracealorganicacidextractinhibitspersistentmethicillinresistantstaphylococcusaureusinvitroandinvivo AT ouchangbo portulacaoleracealorganicacidextractinhibitspersistentmethicillinresistantstaphylococcusaureusinvitroandinvivo |