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Portulaca oleracea L. organic acid extract inhibits persistent methicillin-resistant Staphylococcus aureus in vitro and in vivo

Staphylococcus aureus continues to be one of the most important pathogens capable of causing a wide range of infections in different sites of the body in humans and livestock. With the emergence of methicillin-resistant strains and the introduction of strict laws on antibiotic usage in animals, anti...

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Autores principales: Liu, Gengsong, Liu, Aijing, Yang, Cheng, Zhou, Congcong, Zhou, Qiaoyan, Li, Haizhu, Yang, Hongchun, Mo, Jiahao, Zhang, Zhidan, Li, Gonghe, Si, Hongbin, Ou, Changbo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9874160/
https://www.ncbi.nlm.nih.gov/pubmed/36713183
http://dx.doi.org/10.3389/fmicb.2022.1076154
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author Liu, Gengsong
Liu, Aijing
Yang, Cheng
Zhou, Congcong
Zhou, Qiaoyan
Li, Haizhu
Yang, Hongchun
Mo, Jiahao
Zhang, Zhidan
Li, Gonghe
Si, Hongbin
Ou, Changbo
author_facet Liu, Gengsong
Liu, Aijing
Yang, Cheng
Zhou, Congcong
Zhou, Qiaoyan
Li, Haizhu
Yang, Hongchun
Mo, Jiahao
Zhang, Zhidan
Li, Gonghe
Si, Hongbin
Ou, Changbo
author_sort Liu, Gengsong
collection PubMed
description Staphylococcus aureus continues to be one of the most important pathogens capable of causing a wide range of infections in different sites of the body in humans and livestock. With the emergence of methicillin-resistant strains and the introduction of strict laws on antibiotic usage in animals, antibiotic replacement therapy has become increasingly popular. Previous studies have shown that Portulaca oleracea L. extract exerts a certain degree of bacteriostatic effect, although the active ingredients are unknown. In the present study, the antibacterial activity of the organic acid of P. oleracea (OAPO) against S. aureus was examined using a series of experiments, including the minimum inhibitory concentration, growth curve, and bacteriostasis curve. In vitro antibacterial mechanisms were evaluated based on the integrity and permeability of the cell wall and membrane, scanning electron microscopy, and soluble protein content. A mouse skin wound recovery model was used to verify the antibacterial effects of OAPO on S. aureus in vivo. The results showed that OAPO not only improved skin wound recovery but also decreased the bacterial load in skin wounds. Moreover, the number of inflammatory cells and cytokines decreased in the OAPO-treated groups. In summary, this study reports a botanical extract that can inhibit S. aureus in vitro and in vivo, indicating the potential use of OAPO to prevent and control S. aureus infection in the near future.
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spelling pubmed-98741602023-01-26 Portulaca oleracea L. organic acid extract inhibits persistent methicillin-resistant Staphylococcus aureus in vitro and in vivo Liu, Gengsong Liu, Aijing Yang, Cheng Zhou, Congcong Zhou, Qiaoyan Li, Haizhu Yang, Hongchun Mo, Jiahao Zhang, Zhidan Li, Gonghe Si, Hongbin Ou, Changbo Front Microbiol Microbiology Staphylococcus aureus continues to be one of the most important pathogens capable of causing a wide range of infections in different sites of the body in humans and livestock. With the emergence of methicillin-resistant strains and the introduction of strict laws on antibiotic usage in animals, antibiotic replacement therapy has become increasingly popular. Previous studies have shown that Portulaca oleracea L. extract exerts a certain degree of bacteriostatic effect, although the active ingredients are unknown. In the present study, the antibacterial activity of the organic acid of P. oleracea (OAPO) against S. aureus was examined using a series of experiments, including the minimum inhibitory concentration, growth curve, and bacteriostasis curve. In vitro antibacterial mechanisms were evaluated based on the integrity and permeability of the cell wall and membrane, scanning electron microscopy, and soluble protein content. A mouse skin wound recovery model was used to verify the antibacterial effects of OAPO on S. aureus in vivo. The results showed that OAPO not only improved skin wound recovery but also decreased the bacterial load in skin wounds. Moreover, the number of inflammatory cells and cytokines decreased in the OAPO-treated groups. In summary, this study reports a botanical extract that can inhibit S. aureus in vitro and in vivo, indicating the potential use of OAPO to prevent and control S. aureus infection in the near future. Frontiers Media S.A. 2023-01-11 /pmc/articles/PMC9874160/ /pubmed/36713183 http://dx.doi.org/10.3389/fmicb.2022.1076154 Text en Copyright © 2023 Liu, Liu, Yang, Zhou, Zhou, Li, Yang, Mo, Zhang, Li, Si and Ou. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Liu, Gengsong
Liu, Aijing
Yang, Cheng
Zhou, Congcong
Zhou, Qiaoyan
Li, Haizhu
Yang, Hongchun
Mo, Jiahao
Zhang, Zhidan
Li, Gonghe
Si, Hongbin
Ou, Changbo
Portulaca oleracea L. organic acid extract inhibits persistent methicillin-resistant Staphylococcus aureus in vitro and in vivo
title Portulaca oleracea L. organic acid extract inhibits persistent methicillin-resistant Staphylococcus aureus in vitro and in vivo
title_full Portulaca oleracea L. organic acid extract inhibits persistent methicillin-resistant Staphylococcus aureus in vitro and in vivo
title_fullStr Portulaca oleracea L. organic acid extract inhibits persistent methicillin-resistant Staphylococcus aureus in vitro and in vivo
title_full_unstemmed Portulaca oleracea L. organic acid extract inhibits persistent methicillin-resistant Staphylococcus aureus in vitro and in vivo
title_short Portulaca oleracea L. organic acid extract inhibits persistent methicillin-resistant Staphylococcus aureus in vitro and in vivo
title_sort portulaca oleracea l. organic acid extract inhibits persistent methicillin-resistant staphylococcus aureus in vitro and in vivo
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9874160/
https://www.ncbi.nlm.nih.gov/pubmed/36713183
http://dx.doi.org/10.3389/fmicb.2022.1076154
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