Cargando…

Evaluation of Antibiotic Resistance and adeABC, adeR, adeS Efflux Pump Genes among Foodborne and Clinical Acinetobacter spp. in Türkiye

BACKGROUND: The adeABC efflux pump has a crucial role in the resistance of Acinetobacter baumannii strains to antimicrobial agents; it is encoded by adeABC, adeR, adeS genes. We evaluated antibiotic resistance, efflux pump genes, clonal relationships, and analyzed a probable correlation that can exi...

Descripción completa

Detalles Bibliográficos
Autores principales: Terkuran, Mevhibe, Erginkaya, Zerrin, Konuray, Gözde, Meral, Melda, Ünal, Nevzat, Yaşar, Sertdemir, Köksal, Fatih
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9874188/
https://www.ncbi.nlm.nih.gov/pubmed/36742236
http://dx.doi.org/10.18502/ijph.v51i12.11466
_version_ 1784877751499489280
author Terkuran, Mevhibe
Erginkaya, Zerrin
Konuray, Gözde
Meral, Melda
Ünal, Nevzat
Yaşar, Sertdemir
Köksal, Fatih
author_facet Terkuran, Mevhibe
Erginkaya, Zerrin
Konuray, Gözde
Meral, Melda
Ünal, Nevzat
Yaşar, Sertdemir
Köksal, Fatih
author_sort Terkuran, Mevhibe
collection PubMed
description BACKGROUND: The adeABC efflux pump has a crucial role in the resistance of Acinetobacter baumannii strains to antimicrobial agents; it is encoded by adeABC, adeR, adeS genes. We evaluated antibiotic resistance, efflux pump genes, clonal relationships, and analyzed a probable correlation that can exist between antibiotic resistance and the aforementioned genes. METHODS: We conducted this study on 27 food-originated and 50 human clinical Acinetobacter spp. in Southern Türkiye. MALDI-TOF system and disc diffusion/agar dilution (colistin) methods were used for the identification and antibiotic susceptibility. The efflux pump genes and genetic relatedness of the two groups were investigated by (PCR) and (PFGE) methods. RESULTS: Foodborne A. dijkshoorniae strain was multidrug- resistant (MDR), and none of them resistant to colistin. Most of the clinical isolates (92%) were Extensive-Drug Resistant (XDR); highest resistant to ceftazidime, piperacillin-tazobactam, and imipenem (47, 94%), and were lowest to colistin (7, 14%), respectively. adeABC, and adeR, adeS genes were (23, 85.2%), (9, 33.3%), (27, 100%) and (10, 37.3%), (18, 66.7%) in foodborne strains respectively. These rates were (43, 86%), (48, 96%), (50, 100%), and (34, 68%), (48, 96.7%) in clinical strains respectively. A positive correlation existed between adeA gene positivity and piperacillintazobactam, ceftazidime, gentamycin, imipenem (P=0.048), amikacin (P=0.007) and trimethoprimsulfamethoxazole (P=0.029) resistance in clinical strains. A positive correlation of trimethoprimsulfamethoxazole resistance and adeS gene positivity was seen in foodborne strains (P=0.018). CONCLUSION: Multiple-efflux pump genes rise in parallel to multidrug-resistance in clinical isolates, while susceptible to diverse antibiotics; food may be a potential provenance for the dissemination of adeABC, adeR and adeS genes.
format Online
Article
Text
id pubmed-9874188
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Tehran University of Medical Sciences
record_format MEDLINE/PubMed
spelling pubmed-98741882023-02-02 Evaluation of Antibiotic Resistance and adeABC, adeR, adeS Efflux Pump Genes among Foodborne and Clinical Acinetobacter spp. in Türkiye Terkuran, Mevhibe Erginkaya, Zerrin Konuray, Gözde Meral, Melda Ünal, Nevzat Yaşar, Sertdemir Köksal, Fatih Iran J Public Health Original Article BACKGROUND: The adeABC efflux pump has a crucial role in the resistance of Acinetobacter baumannii strains to antimicrobial agents; it is encoded by adeABC, adeR, adeS genes. We evaluated antibiotic resistance, efflux pump genes, clonal relationships, and analyzed a probable correlation that can exist between antibiotic resistance and the aforementioned genes. METHODS: We conducted this study on 27 food-originated and 50 human clinical Acinetobacter spp. in Southern Türkiye. MALDI-TOF system and disc diffusion/agar dilution (colistin) methods were used for the identification and antibiotic susceptibility. The efflux pump genes and genetic relatedness of the two groups were investigated by (PCR) and (PFGE) methods. RESULTS: Foodborne A. dijkshoorniae strain was multidrug- resistant (MDR), and none of them resistant to colistin. Most of the clinical isolates (92%) were Extensive-Drug Resistant (XDR); highest resistant to ceftazidime, piperacillin-tazobactam, and imipenem (47, 94%), and were lowest to colistin (7, 14%), respectively. adeABC, and adeR, adeS genes were (23, 85.2%), (9, 33.3%), (27, 100%) and (10, 37.3%), (18, 66.7%) in foodborne strains respectively. These rates were (43, 86%), (48, 96%), (50, 100%), and (34, 68%), (48, 96.7%) in clinical strains respectively. A positive correlation existed between adeA gene positivity and piperacillintazobactam, ceftazidime, gentamycin, imipenem (P=0.048), amikacin (P=0.007) and trimethoprimsulfamethoxazole (P=0.029) resistance in clinical strains. A positive correlation of trimethoprimsulfamethoxazole resistance and adeS gene positivity was seen in foodborne strains (P=0.018). CONCLUSION: Multiple-efflux pump genes rise in parallel to multidrug-resistance in clinical isolates, while susceptible to diverse antibiotics; food may be a potential provenance for the dissemination of adeABC, adeR and adeS genes. Tehran University of Medical Sciences 2022-12 /pmc/articles/PMC9874188/ /pubmed/36742236 http://dx.doi.org/10.18502/ijph.v51i12.11466 Text en Copyright © 2022 Terkuran et al. Published by Tehran University of Medical Sciences https://creativecommons.org/licenses/by-nc/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International license (https://creativecommons.org/licenses/by-nc/4.0/). Non-commercial uses of the work are permitted, provided the original work is properly cited.
spellingShingle Original Article
Terkuran, Mevhibe
Erginkaya, Zerrin
Konuray, Gözde
Meral, Melda
Ünal, Nevzat
Yaşar, Sertdemir
Köksal, Fatih
Evaluation of Antibiotic Resistance and adeABC, adeR, adeS Efflux Pump Genes among Foodborne and Clinical Acinetobacter spp. in Türkiye
title Evaluation of Antibiotic Resistance and adeABC, adeR, adeS Efflux Pump Genes among Foodborne and Clinical Acinetobacter spp. in Türkiye
title_full Evaluation of Antibiotic Resistance and adeABC, adeR, adeS Efflux Pump Genes among Foodborne and Clinical Acinetobacter spp. in Türkiye
title_fullStr Evaluation of Antibiotic Resistance and adeABC, adeR, adeS Efflux Pump Genes among Foodborne and Clinical Acinetobacter spp. in Türkiye
title_full_unstemmed Evaluation of Antibiotic Resistance and adeABC, adeR, adeS Efflux Pump Genes among Foodborne and Clinical Acinetobacter spp. in Türkiye
title_short Evaluation of Antibiotic Resistance and adeABC, adeR, adeS Efflux Pump Genes among Foodborne and Clinical Acinetobacter spp. in Türkiye
title_sort evaluation of antibiotic resistance and adeabc, ader, ades efflux pump genes among foodborne and clinical acinetobacter spp. in türkiye
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9874188/
https://www.ncbi.nlm.nih.gov/pubmed/36742236
http://dx.doi.org/10.18502/ijph.v51i12.11466
work_keys_str_mv AT terkuranmevhibe evaluationofantibioticresistanceandadeabcaderadeseffluxpumpgenesamongfoodborneandclinicalacinetobactersppinturkiye
AT erginkayazerrin evaluationofantibioticresistanceandadeabcaderadeseffluxpumpgenesamongfoodborneandclinicalacinetobactersppinturkiye
AT konuraygozde evaluationofantibioticresistanceandadeabcaderadeseffluxpumpgenesamongfoodborneandclinicalacinetobactersppinturkiye
AT meralmelda evaluationofantibioticresistanceandadeabcaderadeseffluxpumpgenesamongfoodborneandclinicalacinetobactersppinturkiye
AT unalnevzat evaluationofantibioticresistanceandadeabcaderadeseffluxpumpgenesamongfoodborneandclinicalacinetobactersppinturkiye
AT yasarsertdemir evaluationofantibioticresistanceandadeabcaderadeseffluxpumpgenesamongfoodborneandclinicalacinetobactersppinturkiye
AT koksalfatih evaluationofantibioticresistanceandadeabcaderadeseffluxpumpgenesamongfoodborneandclinicalacinetobactersppinturkiye