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PaR1 secreted by the type IX secretion system is a protective antigen of Riemerella anatipestifer

Riemerella anatipestifer mainly infects domestic ducks, geese, turkeys, and other birds, and causes considerable economic losses to the global duck industry. Previous studies have shown that concentrated cell-free culture filtrates of R. anatipestifer induce highly significant protection against hom...

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Autores principales: Wang, Jialing, Chen, Yan, He, Xiaohua, Du, Xiaoli, Gao, Yongheng, Shan, Xinggen, Hu, Zhiqun, Hu, Qinghai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9874225/
https://www.ncbi.nlm.nih.gov/pubmed/36713192
http://dx.doi.org/10.3389/fmicb.2022.1082712
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author Wang, Jialing
Chen, Yan
He, Xiaohua
Du, Xiaoli
Gao, Yongheng
Shan, Xinggen
Hu, Zhiqun
Hu, Qinghai
author_facet Wang, Jialing
Chen, Yan
He, Xiaohua
Du, Xiaoli
Gao, Yongheng
Shan, Xinggen
Hu, Zhiqun
Hu, Qinghai
author_sort Wang, Jialing
collection PubMed
description Riemerella anatipestifer mainly infects domestic ducks, geese, turkeys, and other birds, and causes considerable economic losses to the global duck industry. Previous studies have shown that concentrated cell-free culture filtrates of R. anatipestifer induce highly significant protection against homologous challenge. In this study, 12 immunogenic proteins were identified in the culture supernatant of R. anatipestifer strain Yb2 with immunoproteomic analysis. Of these, three immunogenic proteins, AS87_RS06600 (designated “PaR1” in this study), AS87_RS09020, and AS87_RS09965, which appeared in more than three spots on the western-blotted membrane, were expressed in Escherichia coli and purified. Animal experiments showed that the recombinant PaR1 (rPaR1) protein protected 41.67% of immunized ducklings against challenge with virulent Yb2, whereas rAS87_RS09020 or rAS87_RS09965 did not, and that ducklings immunized once with rPaR1 were 20, 40, and 0% protected from challenge with R. anatipestifer strains WJ4 (serotype 1), Yb2 (serotype 2), and HXb2 (serotype 10), respectively. In addition, rPaR1 immunized rabbit serum showed bactericidal activity against strain Yb2 at a titer of 1:8. These results indicate that rPaR1 of strain Yb2 protects against homologous challenge. Amino acid homology analysis show that PaR1 is a non-serotype-specific protein among different R. anatipestifer serotypes. Furthermore, PaR1 is mainly secreted outside the cell through the T9SS. Overall, our results demonstrate that R. anatipestifer PaR1 is a non-serotype-specific protective protein secreted by the T9SS.
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spelling pubmed-98742252023-01-26 PaR1 secreted by the type IX secretion system is a protective antigen of Riemerella anatipestifer Wang, Jialing Chen, Yan He, Xiaohua Du, Xiaoli Gao, Yongheng Shan, Xinggen Hu, Zhiqun Hu, Qinghai Front Microbiol Microbiology Riemerella anatipestifer mainly infects domestic ducks, geese, turkeys, and other birds, and causes considerable economic losses to the global duck industry. Previous studies have shown that concentrated cell-free culture filtrates of R. anatipestifer induce highly significant protection against homologous challenge. In this study, 12 immunogenic proteins were identified in the culture supernatant of R. anatipestifer strain Yb2 with immunoproteomic analysis. Of these, three immunogenic proteins, AS87_RS06600 (designated “PaR1” in this study), AS87_RS09020, and AS87_RS09965, which appeared in more than three spots on the western-blotted membrane, were expressed in Escherichia coli and purified. Animal experiments showed that the recombinant PaR1 (rPaR1) protein protected 41.67% of immunized ducklings against challenge with virulent Yb2, whereas rAS87_RS09020 or rAS87_RS09965 did not, and that ducklings immunized once with rPaR1 were 20, 40, and 0% protected from challenge with R. anatipestifer strains WJ4 (serotype 1), Yb2 (serotype 2), and HXb2 (serotype 10), respectively. In addition, rPaR1 immunized rabbit serum showed bactericidal activity against strain Yb2 at a titer of 1:8. These results indicate that rPaR1 of strain Yb2 protects against homologous challenge. Amino acid homology analysis show that PaR1 is a non-serotype-specific protein among different R. anatipestifer serotypes. Furthermore, PaR1 is mainly secreted outside the cell through the T9SS. Overall, our results demonstrate that R. anatipestifer PaR1 is a non-serotype-specific protective protein secreted by the T9SS. Frontiers Media S.A. 2023-01-11 /pmc/articles/PMC9874225/ /pubmed/36713192 http://dx.doi.org/10.3389/fmicb.2022.1082712 Text en Copyright © 2023 Wang, Chen, He, Du, Gao, Shan, Hu and Hu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Wang, Jialing
Chen, Yan
He, Xiaohua
Du, Xiaoli
Gao, Yongheng
Shan, Xinggen
Hu, Zhiqun
Hu, Qinghai
PaR1 secreted by the type IX secretion system is a protective antigen of Riemerella anatipestifer
title PaR1 secreted by the type IX secretion system is a protective antigen of Riemerella anatipestifer
title_full PaR1 secreted by the type IX secretion system is a protective antigen of Riemerella anatipestifer
title_fullStr PaR1 secreted by the type IX secretion system is a protective antigen of Riemerella anatipestifer
title_full_unstemmed PaR1 secreted by the type IX secretion system is a protective antigen of Riemerella anatipestifer
title_short PaR1 secreted by the type IX secretion system is a protective antigen of Riemerella anatipestifer
title_sort par1 secreted by the type ix secretion system is a protective antigen of riemerella anatipestifer
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9874225/
https://www.ncbi.nlm.nih.gov/pubmed/36713192
http://dx.doi.org/10.3389/fmicb.2022.1082712
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