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Fungicide sensitivity levels in the Lithuanian Zymoseptoria tritici population in 2021

Zymoseptoria tritici causes the disease known as septoria leaf blotch in winter wheat and is a major factor in yield loss worldwide. Farmers are inclined to use fungicides to protect their crops; however, the efficacy of these measures is rapidly decreasing due to the natural mechanisms of mutation...

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Autores principales: Lavrukaitė, Karolina, Heick, Thies M., Ramanauskienė, Jūratė, Armonienė, Rita, Ronis, Antanas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9875010/
https://www.ncbi.nlm.nih.gov/pubmed/36714691
http://dx.doi.org/10.3389/fpls.2022.1075038
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author Lavrukaitė, Karolina
Heick, Thies M.
Ramanauskienė, Jūratė
Armonienė, Rita
Ronis, Antanas
author_facet Lavrukaitė, Karolina
Heick, Thies M.
Ramanauskienė, Jūratė
Armonienė, Rita
Ronis, Antanas
author_sort Lavrukaitė, Karolina
collection PubMed
description Zymoseptoria tritici causes the disease known as septoria leaf blotch in winter wheat and is a major factor in yield loss worldwide. Farmers are inclined to use fungicides to protect their crops; however, the efficacy of these measures is rapidly decreasing due to the natural mechanisms of mutation emergence in pathogen populations. Increasing fungicide resistance is being recorded worldwide, therefore, screening of the current situation in Lithuania is essential to determine the subsequent steps of crop protection strategies. In this study, in vitro fungicide sensitivity tests, mutation detection, and field experiments were carried out. The mean EC(50) values for prothioconazole-desthio and mefentrifluconazole were 0.14 and 0.28 mg/l, respectively. Increased frequency of the mutation S524T, linked to DMIs resistance, was observed. Results revealed that the dominant point mutation in the gene CYP51 was I381V, and the most frequent CYP51 haplotype was D13 (V136C, I381V, Y461H, S524T). The mutation G143A, linked to QoI resistance, was detected in ¾ of the population. Mutations conferring resistance to SDHIs were not detected in single pycnidium isolates. Two-year field experiments likewise showed no decline in field efficacy of SDHI fungicide in Lithuania. Moreover, the baseline sensitivity of the Lithuanian Z. tritici population to QiI fungicide fenpicoxamid was established. The findings of this study provide an update on the current status of fungicide resistance in the Lithuanian Z. tritici population.
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spelling pubmed-98750102023-01-26 Fungicide sensitivity levels in the Lithuanian Zymoseptoria tritici population in 2021 Lavrukaitė, Karolina Heick, Thies M. Ramanauskienė, Jūratė Armonienė, Rita Ronis, Antanas Front Plant Sci Plant Science Zymoseptoria tritici causes the disease known as septoria leaf blotch in winter wheat and is a major factor in yield loss worldwide. Farmers are inclined to use fungicides to protect their crops; however, the efficacy of these measures is rapidly decreasing due to the natural mechanisms of mutation emergence in pathogen populations. Increasing fungicide resistance is being recorded worldwide, therefore, screening of the current situation in Lithuania is essential to determine the subsequent steps of crop protection strategies. In this study, in vitro fungicide sensitivity tests, mutation detection, and field experiments were carried out. The mean EC(50) values for prothioconazole-desthio and mefentrifluconazole were 0.14 and 0.28 mg/l, respectively. Increased frequency of the mutation S524T, linked to DMIs resistance, was observed. Results revealed that the dominant point mutation in the gene CYP51 was I381V, and the most frequent CYP51 haplotype was D13 (V136C, I381V, Y461H, S524T). The mutation G143A, linked to QoI resistance, was detected in ¾ of the population. Mutations conferring resistance to SDHIs were not detected in single pycnidium isolates. Two-year field experiments likewise showed no decline in field efficacy of SDHI fungicide in Lithuania. Moreover, the baseline sensitivity of the Lithuanian Z. tritici population to QiI fungicide fenpicoxamid was established. The findings of this study provide an update on the current status of fungicide resistance in the Lithuanian Z. tritici population. Frontiers Media S.A. 2023-01-11 /pmc/articles/PMC9875010/ /pubmed/36714691 http://dx.doi.org/10.3389/fpls.2022.1075038 Text en Copyright © 2023 Lavrukaitė, Heick, Ramanauskienė, Armonienė and Ronis https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Lavrukaitė, Karolina
Heick, Thies M.
Ramanauskienė, Jūratė
Armonienė, Rita
Ronis, Antanas
Fungicide sensitivity levels in the Lithuanian Zymoseptoria tritici population in 2021
title Fungicide sensitivity levels in the Lithuanian Zymoseptoria tritici population in 2021
title_full Fungicide sensitivity levels in the Lithuanian Zymoseptoria tritici population in 2021
title_fullStr Fungicide sensitivity levels in the Lithuanian Zymoseptoria tritici population in 2021
title_full_unstemmed Fungicide sensitivity levels in the Lithuanian Zymoseptoria tritici population in 2021
title_short Fungicide sensitivity levels in the Lithuanian Zymoseptoria tritici population in 2021
title_sort fungicide sensitivity levels in the lithuanian zymoseptoria tritici population in 2021
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9875010/
https://www.ncbi.nlm.nih.gov/pubmed/36714691
http://dx.doi.org/10.3389/fpls.2022.1075038
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