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Biosynthesis of aliphatic plastic monomers with amino residues in Yarrowia lipolytica
Introduciton: The α,ω-diamines (NH(2)-(CH(2))(n)-NH(2)) and ω -amino fatty acids (NH(2)-(CH(2))(n)-COOH) have been widely used as building blocks in polymerindustries. Medium- to long-chain (C(8) to C(18)) fatty acid monomers with amino residues are almost exclusively produced via chemical processes...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9875067/ https://www.ncbi.nlm.nih.gov/pubmed/36714625 http://dx.doi.org/10.3389/fbioe.2022.825576 |
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author | Park, Gyuyeon Kim, Ye Chan Jang, Minjeong Park, Hyuna Lee, Hong-Weon Jeon, Wooyoung Kim, Byung-Gee Choi, Kwon-Young Ahn, Jungoh |
author_facet | Park, Gyuyeon Kim, Ye Chan Jang, Minjeong Park, Hyuna Lee, Hong-Weon Jeon, Wooyoung Kim, Byung-Gee Choi, Kwon-Young Ahn, Jungoh |
author_sort | Park, Gyuyeon |
collection | PubMed |
description | Introduciton: The α,ω-diamines (NH(2)-(CH(2))(n)-NH(2)) and ω -amino fatty acids (NH(2)-(CH(2))(n)-COOH) have been widely used as building blocks in polymerindustries. Medium- to long-chain (C(8) to C(18)) fatty acid monomers with amino residues are almost exclusively produced via chemical processes that generate hazardous waste and induce severe environmental problems, such as global warming and pollution. Here, we present the construction platformstrains of Yarrowia lipolytica a cheese-ripening yeast, for direct biotransformation of hydrocarbons into medium- to long-chain α,ω-diamines and ωamino fatty acids using metabolic engineering of endogenous fatty acid ω- and β-oxidation pathways and introducing heterologous ω-transaminase in Y. lipolytica. Methods: We deleted six genes encoding the acyl-CoA oxidase (ACO1–6) and four fatty aldehyde dehydrogenase genes (FALDH1-4), which catalyze fatty acid β-oxidation and downstream oxidation of fatty aldehydes in Y. lipolytica, respectively. The ω-transaminase from Chromobacterium violaceum DSM30191 was introduced into the genome of the ΔPOX ΔFALDH strain under the control of Y. lipolytica-derived EXP1 promoters. Results and Discussion: The ΔPOX ΔFALDH strains with ω-CvTA successfully accumulated the corresponding C12 αω-diamines into a shaking culture medium with dodecane or dodecanol. In addition, these strains accumulated C(12) ω-amino fatty acids from dodecanoic acid. With the commercially available α,ω-diacid bioprocess, this yeast biosynthesis producing medium- and longchain α,ω-diamines and ω-amino fatty acids could complete the yeast platform technology generating all medium- and long-chain aliphatic polyamide monomers, α,ω-biofunctionalized with one or both carboxylic acid and amino residues. |
format | Online Article Text |
id | pubmed-9875067 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-98750672023-01-26 Biosynthesis of aliphatic plastic monomers with amino residues in Yarrowia lipolytica Park, Gyuyeon Kim, Ye Chan Jang, Minjeong Park, Hyuna Lee, Hong-Weon Jeon, Wooyoung Kim, Byung-Gee Choi, Kwon-Young Ahn, Jungoh Front Bioeng Biotechnol Bioengineering and Biotechnology Introduciton: The α,ω-diamines (NH(2)-(CH(2))(n)-NH(2)) and ω -amino fatty acids (NH(2)-(CH(2))(n)-COOH) have been widely used as building blocks in polymerindustries. Medium- to long-chain (C(8) to C(18)) fatty acid monomers with amino residues are almost exclusively produced via chemical processes that generate hazardous waste and induce severe environmental problems, such as global warming and pollution. Here, we present the construction platformstrains of Yarrowia lipolytica a cheese-ripening yeast, for direct biotransformation of hydrocarbons into medium- to long-chain α,ω-diamines and ωamino fatty acids using metabolic engineering of endogenous fatty acid ω- and β-oxidation pathways and introducing heterologous ω-transaminase in Y. lipolytica. Methods: We deleted six genes encoding the acyl-CoA oxidase (ACO1–6) and four fatty aldehyde dehydrogenase genes (FALDH1-4), which catalyze fatty acid β-oxidation and downstream oxidation of fatty aldehydes in Y. lipolytica, respectively. The ω-transaminase from Chromobacterium violaceum DSM30191 was introduced into the genome of the ΔPOX ΔFALDH strain under the control of Y. lipolytica-derived EXP1 promoters. Results and Discussion: The ΔPOX ΔFALDH strains with ω-CvTA successfully accumulated the corresponding C12 αω-diamines into a shaking culture medium with dodecane or dodecanol. In addition, these strains accumulated C(12) ω-amino fatty acids from dodecanoic acid. With the commercially available α,ω-diacid bioprocess, this yeast biosynthesis producing medium- and longchain α,ω-diamines and ω-amino fatty acids could complete the yeast platform technology generating all medium- and long-chain aliphatic polyamide monomers, α,ω-biofunctionalized with one or both carboxylic acid and amino residues. Frontiers Media S.A. 2023-01-11 /pmc/articles/PMC9875067/ /pubmed/36714625 http://dx.doi.org/10.3389/fbioe.2022.825576 Text en Copyright © 2023 Park, Kim, Jang, Park, Lee, Jeon, Kim, Choi and Ahn. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Park, Gyuyeon Kim, Ye Chan Jang, Minjeong Park, Hyuna Lee, Hong-Weon Jeon, Wooyoung Kim, Byung-Gee Choi, Kwon-Young Ahn, Jungoh Biosynthesis of aliphatic plastic monomers with amino residues in Yarrowia lipolytica |
title | Biosynthesis of aliphatic plastic monomers with amino residues in Yarrowia lipolytica
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title_full | Biosynthesis of aliphatic plastic monomers with amino residues in Yarrowia lipolytica
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title_fullStr | Biosynthesis of aliphatic plastic monomers with amino residues in Yarrowia lipolytica
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title_full_unstemmed | Biosynthesis of aliphatic plastic monomers with amino residues in Yarrowia lipolytica
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title_short | Biosynthesis of aliphatic plastic monomers with amino residues in Yarrowia lipolytica
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title_sort | biosynthesis of aliphatic plastic monomers with amino residues in yarrowia lipolytica |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9875067/ https://www.ncbi.nlm.nih.gov/pubmed/36714625 http://dx.doi.org/10.3389/fbioe.2022.825576 |
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