Construction of PARPi Resistance-related Competing Endogenous RNA Network

Objective: Ovarian cancer is a kind of common gynecological malignancy in women. PARP inhibitors (PARPi) have been approved for ovarian cancer treatment. However, the primary and acquired resistance have limited the application of PARPi. The mechanisms remain to be elucidated. Methods: In this study...

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Autores principales: Kong, Lili, Xu, Jiaqi, Yu, Lijun, Liu, Shuo, Liu, Zongjian, Xiang, Juanjuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bentham Science Publishers 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9875538/
https://www.ncbi.nlm.nih.gov/pubmed/36777878
http://dx.doi.org/10.2174/1389202923666220527114108
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author Kong, Lili
Xu, Jiaqi
Yu, Lijun
Liu, Shuo
Liu, Zongjian
Xiang, Juanjuan
author_facet Kong, Lili
Xu, Jiaqi
Yu, Lijun
Liu, Shuo
Liu, Zongjian
Xiang, Juanjuan
author_sort Kong, Lili
collection PubMed
description Objective: Ovarian cancer is a kind of common gynecological malignancy in women. PARP inhibitors (PARPi) have been approved for ovarian cancer treatment. However, the primary and acquired resistance have limited the application of PARPi. The mechanisms remain to be elucidated. Methods: In this study, we characterized the expression profiles of mRNA and nonconding RNAs (ncRNAs) and constructed the regulatory networks based on RNA sequencing in PARPi Olaparib-induced ovarian cancer cells. Results: We found that the functions of the differentially expressed genes were enriched in “PI3K/AKT signaling pathway,” “MAPK signaling pathway” and “metabolic process”. The functions of DELs (cis) were enriched in “Human papillomavirus infection”“tight junction” “MAPK signaling pathway”. As the central regulator of ceRNAs, the differentially expressed miRNAs were enriched in “Human papillomavirus infection” “MAPK signaling pathway” “Ras signaling pathway”. According to the degree of interaction, we identified 3 lncRNAs, 2 circRNAs, 7 miRNAs, and 12 mRNA as the key regulatory ceRNA axis, in which miR-320b was the important mediator. Conclusion: Here, we revealed the key regulatory lncRNA (circRNA)-miRNA-mRNA axis and their involved pathways in the PARPi resistant ovarian cancer cells. These findings provide new insights into exploring the ceRNA regulatory networks and developing new targets for PARPi resistance.
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spelling pubmed-98755382023-02-11 Construction of PARPi Resistance-related Competing Endogenous RNA Network Kong, Lili Xu, Jiaqi Yu, Lijun Liu, Shuo Liu, Zongjian Xiang, Juanjuan Curr Genomics Genetics & Genomics Objective: Ovarian cancer is a kind of common gynecological malignancy in women. PARP inhibitors (PARPi) have been approved for ovarian cancer treatment. However, the primary and acquired resistance have limited the application of PARPi. The mechanisms remain to be elucidated. Methods: In this study, we characterized the expression profiles of mRNA and nonconding RNAs (ncRNAs) and constructed the regulatory networks based on RNA sequencing in PARPi Olaparib-induced ovarian cancer cells. Results: We found that the functions of the differentially expressed genes were enriched in “PI3K/AKT signaling pathway,” “MAPK signaling pathway” and “metabolic process”. The functions of DELs (cis) were enriched in “Human papillomavirus infection”“tight junction” “MAPK signaling pathway”. As the central regulator of ceRNAs, the differentially expressed miRNAs were enriched in “Human papillomavirus infection” “MAPK signaling pathway” “Ras signaling pathway”. According to the degree of interaction, we identified 3 lncRNAs, 2 circRNAs, 7 miRNAs, and 12 mRNA as the key regulatory ceRNA axis, in which miR-320b was the important mediator. Conclusion: Here, we revealed the key regulatory lncRNA (circRNA)-miRNA-mRNA axis and their involved pathways in the PARPi resistant ovarian cancer cells. These findings provide new insights into exploring the ceRNA regulatory networks and developing new targets for PARPi resistance. Bentham Science Publishers 2022-08-11 2022-08-11 /pmc/articles/PMC9875538/ /pubmed/36777878 http://dx.doi.org/10.2174/1389202923666220527114108 Text en © 2022 Bentham Science Publishers https://creativecommons.org/licenses/by-nc/4.0/ This is an open access article licensed under the terms of the Creative Commons Attribution-Non-Commercial 4.0 International Public License (CC BY-NC 4.0) (https://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
spellingShingle Genetics & Genomics
Kong, Lili
Xu, Jiaqi
Yu, Lijun
Liu, Shuo
Liu, Zongjian
Xiang, Juanjuan
Construction of PARPi Resistance-related Competing Endogenous RNA Network
title Construction of PARPi Resistance-related Competing Endogenous RNA Network
title_full Construction of PARPi Resistance-related Competing Endogenous RNA Network
title_fullStr Construction of PARPi Resistance-related Competing Endogenous RNA Network
title_full_unstemmed Construction of PARPi Resistance-related Competing Endogenous RNA Network
title_short Construction of PARPi Resistance-related Competing Endogenous RNA Network
title_sort construction of parpi resistance-related competing endogenous rna network
topic Genetics & Genomics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9875538/
https://www.ncbi.nlm.nih.gov/pubmed/36777878
http://dx.doi.org/10.2174/1389202923666220527114108
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