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Characterisation of human milk bacterial DNA profiles in a small cohort of Australian women in relation to infant and maternal factors

Human milk is composed of complex microbial and non-microbial components that shape the infant gut microbiome. Although several maternal and infant factors have been associated with human milk microbiota, no study has investigated this in an Australian population. Therefore, we aimed to investigate...

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Autores principales: Sindi, Azhar S., Cheema, Ali S., Trevenen, Michelle L., Geddes, Donna T., Payne, Matthew S., Stinson, Lisa F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9876237/
https://www.ncbi.nlm.nih.gov/pubmed/36696407
http://dx.doi.org/10.1371/journal.pone.0280960
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author Sindi, Azhar S.
Cheema, Ali S.
Trevenen, Michelle L.
Geddes, Donna T.
Payne, Matthew S.
Stinson, Lisa F.
author_facet Sindi, Azhar S.
Cheema, Ali S.
Trevenen, Michelle L.
Geddes, Donna T.
Payne, Matthew S.
Stinson, Lisa F.
author_sort Sindi, Azhar S.
collection PubMed
description Human milk is composed of complex microbial and non-microbial components that shape the infant gut microbiome. Although several maternal and infant factors have been associated with human milk microbiota, no study has investigated this in an Australian population. Therefore, we aimed to investigate associations between human milk bacterial composition of Australian women and maternal factors (body mass index (BMI), mode of delivery, breast pump use, allergy, parity) and infant factors (sex, mode of feeding, pacifier use, and introduction of solids). Full-length 16S rRNA gene sequencing was used to characterise milk bacterial DNA profiles. Milk from mothers with a normal BMI had a higher relative abundance of Streptococcus australis than that of underweight mothers, while milk from overweight mothers had a higher relative abundance of Streptococcus salivarius compared with underweight and obese mothers. Mothers who delivered vaginally had a higher relative abundance of Streptococcus mitis in their milk compared to those who delivered via emergency caesarean section. Milk of mothers who used a breast pump had a higher relative abundance of Staphylococcus epidermidis and Streptococcus parasanguinis. Milk of mothers whose infants used a pacifier had a higher relative abundance of S. australis and Streptococcus gwangjuense. Maternal BMI, mode of delivery, breast pump use, and infant pacifier use are associated with the bacterial composition of human milk in an Australian cohort. The data from this pilot study suggests that both mother and infant can contribute to the human milk microbiome.
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spelling pubmed-98762372023-01-26 Characterisation of human milk bacterial DNA profiles in a small cohort of Australian women in relation to infant and maternal factors Sindi, Azhar S. Cheema, Ali S. Trevenen, Michelle L. Geddes, Donna T. Payne, Matthew S. Stinson, Lisa F. PLoS One Research Article Human milk is composed of complex microbial and non-microbial components that shape the infant gut microbiome. Although several maternal and infant factors have been associated with human milk microbiota, no study has investigated this in an Australian population. Therefore, we aimed to investigate associations between human milk bacterial composition of Australian women and maternal factors (body mass index (BMI), mode of delivery, breast pump use, allergy, parity) and infant factors (sex, mode of feeding, pacifier use, and introduction of solids). Full-length 16S rRNA gene sequencing was used to characterise milk bacterial DNA profiles. Milk from mothers with a normal BMI had a higher relative abundance of Streptococcus australis than that of underweight mothers, while milk from overweight mothers had a higher relative abundance of Streptococcus salivarius compared with underweight and obese mothers. Mothers who delivered vaginally had a higher relative abundance of Streptococcus mitis in their milk compared to those who delivered via emergency caesarean section. Milk of mothers who used a breast pump had a higher relative abundance of Staphylococcus epidermidis and Streptococcus parasanguinis. Milk of mothers whose infants used a pacifier had a higher relative abundance of S. australis and Streptococcus gwangjuense. Maternal BMI, mode of delivery, breast pump use, and infant pacifier use are associated with the bacterial composition of human milk in an Australian cohort. The data from this pilot study suggests that both mother and infant can contribute to the human milk microbiome. Public Library of Science 2023-01-25 /pmc/articles/PMC9876237/ /pubmed/36696407 http://dx.doi.org/10.1371/journal.pone.0280960 Text en © 2023 Sindi et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Sindi, Azhar S.
Cheema, Ali S.
Trevenen, Michelle L.
Geddes, Donna T.
Payne, Matthew S.
Stinson, Lisa F.
Characterisation of human milk bacterial DNA profiles in a small cohort of Australian women in relation to infant and maternal factors
title Characterisation of human milk bacterial DNA profiles in a small cohort of Australian women in relation to infant and maternal factors
title_full Characterisation of human milk bacterial DNA profiles in a small cohort of Australian women in relation to infant and maternal factors
title_fullStr Characterisation of human milk bacterial DNA profiles in a small cohort of Australian women in relation to infant and maternal factors
title_full_unstemmed Characterisation of human milk bacterial DNA profiles in a small cohort of Australian women in relation to infant and maternal factors
title_short Characterisation of human milk bacterial DNA profiles in a small cohort of Australian women in relation to infant and maternal factors
title_sort characterisation of human milk bacterial dna profiles in a small cohort of australian women in relation to infant and maternal factors
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9876237/
https://www.ncbi.nlm.nih.gov/pubmed/36696407
http://dx.doi.org/10.1371/journal.pone.0280960
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