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LINC01278 Induces Autophagy to Inhibit Tumour Progression by Suppressing the mTOR Signalling Pathway

Uveal melanoma (UM) is an aggressive intraocular malignant tumour that is closely related to autophagic dysfunction. We aimed to identify autophagy-related long noncoding RNAs (lncRNAs) to elucidate the molecular mechanism of UM. Here, we show that LINC01278 is a new potential biomarker with clinica...

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Autores principales: Liu, Bo, Yao, Xueting, Zhang, Chaoyang, Li, Wenzhe, Wang, Yanan, Liao, Qianling, Li, Ziwei, Huang, Qinying, Zhang, Yanchen, Wu, Wencan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9876672/
https://www.ncbi.nlm.nih.gov/pubmed/36713034
http://dx.doi.org/10.1155/2023/8994901
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author Liu, Bo
Yao, Xueting
Zhang, Chaoyang
Li, Wenzhe
Wang, Yanan
Liao, Qianling
Li, Ziwei
Huang, Qinying
Zhang, Yanchen
Wu, Wencan
author_facet Liu, Bo
Yao, Xueting
Zhang, Chaoyang
Li, Wenzhe
Wang, Yanan
Liao, Qianling
Li, Ziwei
Huang, Qinying
Zhang, Yanchen
Wu, Wencan
author_sort Liu, Bo
collection PubMed
description Uveal melanoma (UM) is an aggressive intraocular malignant tumour that is closely related to autophagic dysfunction. We aimed to identify autophagy-related long noncoding RNAs (lncRNAs) to elucidate the molecular mechanism of UM. Here, we show that LINC01278 is a new potential biomarker with clinical prognostic value in UM through bioinformatics analysis. Application of an autophagy inhibitor (3-MA) and an autophagy agonist (MG-132) indicated that LINC01278 can inhibit UM cell proliferation, migration, and invasion by inducing autophagy. A xenograft nude mouse model was used to examine the tumorigenesis of UM cells in vivo. Mechanistically, LINC01278 can inhibit the mTOR signalling pathway to activate autophagy, as shown by experiments with an mTOR agonist (MHY1485) and mTOR inhibitor (rapamycin) treatment. Our findings indicate that LINC01278 functions as a tumour suppressor by inhibiting the mTOR signalling pathway to induce autophagy. Targeting the LINC01278-mTOR axis might be a novel and promising therapeutic approach for UM.
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spelling pubmed-98766722023-01-26 LINC01278 Induces Autophagy to Inhibit Tumour Progression by Suppressing the mTOR Signalling Pathway Liu, Bo Yao, Xueting Zhang, Chaoyang Li, Wenzhe Wang, Yanan Liao, Qianling Li, Ziwei Huang, Qinying Zhang, Yanchen Wu, Wencan Oxid Med Cell Longev Research Article Uveal melanoma (UM) is an aggressive intraocular malignant tumour that is closely related to autophagic dysfunction. We aimed to identify autophagy-related long noncoding RNAs (lncRNAs) to elucidate the molecular mechanism of UM. Here, we show that LINC01278 is a new potential biomarker with clinical prognostic value in UM through bioinformatics analysis. Application of an autophagy inhibitor (3-MA) and an autophagy agonist (MG-132) indicated that LINC01278 can inhibit UM cell proliferation, migration, and invasion by inducing autophagy. A xenograft nude mouse model was used to examine the tumorigenesis of UM cells in vivo. Mechanistically, LINC01278 can inhibit the mTOR signalling pathway to activate autophagy, as shown by experiments with an mTOR agonist (MHY1485) and mTOR inhibitor (rapamycin) treatment. Our findings indicate that LINC01278 functions as a tumour suppressor by inhibiting the mTOR signalling pathway to induce autophagy. Targeting the LINC01278-mTOR axis might be a novel and promising therapeutic approach for UM. Hindawi 2023-01-18 /pmc/articles/PMC9876672/ /pubmed/36713034 http://dx.doi.org/10.1155/2023/8994901 Text en Copyright © 2023 Bo Liu et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Liu, Bo
Yao, Xueting
Zhang, Chaoyang
Li, Wenzhe
Wang, Yanan
Liao, Qianling
Li, Ziwei
Huang, Qinying
Zhang, Yanchen
Wu, Wencan
LINC01278 Induces Autophagy to Inhibit Tumour Progression by Suppressing the mTOR Signalling Pathway
title LINC01278 Induces Autophagy to Inhibit Tumour Progression by Suppressing the mTOR Signalling Pathway
title_full LINC01278 Induces Autophagy to Inhibit Tumour Progression by Suppressing the mTOR Signalling Pathway
title_fullStr LINC01278 Induces Autophagy to Inhibit Tumour Progression by Suppressing the mTOR Signalling Pathway
title_full_unstemmed LINC01278 Induces Autophagy to Inhibit Tumour Progression by Suppressing the mTOR Signalling Pathway
title_short LINC01278 Induces Autophagy to Inhibit Tumour Progression by Suppressing the mTOR Signalling Pathway
title_sort linc01278 induces autophagy to inhibit tumour progression by suppressing the mtor signalling pathway
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9876672/
https://www.ncbi.nlm.nih.gov/pubmed/36713034
http://dx.doi.org/10.1155/2023/8994901
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