Cargando…

In Vitro Insulin Resistance Model: A Recent Update

Insulin resistance, which affects insulin-sensitive tissues, including adipose tissues, skeletal muscle, and the liver, is the central pathophysiological mechanism underlying type 2 diabetes progression. Decreased glucose uptake in insulin-sensitive tissues disrupts insulin signaling pathways, parti...

Descripción completa

Detalles Bibliográficos
Autores principales: Yudhani, Ratih D., Sari, Yulia, Nugrahaningsih, Dwi A. A., Sholikhah, Eti N., Rochmanti, Maftuchah, Purba, Abdul K. R., Khotimah, Husnul, Nugrahenny, Dian, Mustofa, Mustofa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9876677/
https://www.ncbi.nlm.nih.gov/pubmed/36714242
http://dx.doi.org/10.1155/2023/1964732
_version_ 1784878215322402816
author Yudhani, Ratih D.
Sari, Yulia
Nugrahaningsih, Dwi A. A.
Sholikhah, Eti N.
Rochmanti, Maftuchah
Purba, Abdul K. R.
Khotimah, Husnul
Nugrahenny, Dian
Mustofa, Mustofa
author_facet Yudhani, Ratih D.
Sari, Yulia
Nugrahaningsih, Dwi A. A.
Sholikhah, Eti N.
Rochmanti, Maftuchah
Purba, Abdul K. R.
Khotimah, Husnul
Nugrahenny, Dian
Mustofa, Mustofa
author_sort Yudhani, Ratih D.
collection PubMed
description Insulin resistance, which affects insulin-sensitive tissues, including adipose tissues, skeletal muscle, and the liver, is the central pathophysiological mechanism underlying type 2 diabetes progression. Decreased glucose uptake in insulin-sensitive tissues disrupts insulin signaling pathways, particularly the PI3K/Akt pathway. An in vitro model is appropriate for studying the cellular and molecular mechanisms underlying insulin resistance because it is easy to maintain and the results can be easily reproduced. The application of cell-based models for exploring the pathogenesis of diabetes and insulin resistance as well as for developing drugs for these conditions is well known. However, a comprehensive review of in vitro insulin resistance models is lacking. Therefore, this review was conducted to provide a comprehensive overview and summary of the latest in vitro insulin resistance models, particularly 3T3-L1 (preadipocyte), C2C12 (skeletal muscle), and HepG2 (liver) cell lines induced with palmitic acid, high glucose, or chronic exposure to insulin.
format Online
Article
Text
id pubmed-9876677
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-98766772023-01-26 In Vitro Insulin Resistance Model: A Recent Update Yudhani, Ratih D. Sari, Yulia Nugrahaningsih, Dwi A. A. Sholikhah, Eti N. Rochmanti, Maftuchah Purba, Abdul K. R. Khotimah, Husnul Nugrahenny, Dian Mustofa, Mustofa J Obes Review Article Insulin resistance, which affects insulin-sensitive tissues, including adipose tissues, skeletal muscle, and the liver, is the central pathophysiological mechanism underlying type 2 diabetes progression. Decreased glucose uptake in insulin-sensitive tissues disrupts insulin signaling pathways, particularly the PI3K/Akt pathway. An in vitro model is appropriate for studying the cellular and molecular mechanisms underlying insulin resistance because it is easy to maintain and the results can be easily reproduced. The application of cell-based models for exploring the pathogenesis of diabetes and insulin resistance as well as for developing drugs for these conditions is well known. However, a comprehensive review of in vitro insulin resistance models is lacking. Therefore, this review was conducted to provide a comprehensive overview and summary of the latest in vitro insulin resistance models, particularly 3T3-L1 (preadipocyte), C2C12 (skeletal muscle), and HepG2 (liver) cell lines induced with palmitic acid, high glucose, or chronic exposure to insulin. Hindawi 2023-01-18 /pmc/articles/PMC9876677/ /pubmed/36714242 http://dx.doi.org/10.1155/2023/1964732 Text en Copyright © 2023 Ratih D. Yudhani et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review Article
Yudhani, Ratih D.
Sari, Yulia
Nugrahaningsih, Dwi A. A.
Sholikhah, Eti N.
Rochmanti, Maftuchah
Purba, Abdul K. R.
Khotimah, Husnul
Nugrahenny, Dian
Mustofa, Mustofa
In Vitro Insulin Resistance Model: A Recent Update
title In Vitro Insulin Resistance Model: A Recent Update
title_full In Vitro Insulin Resistance Model: A Recent Update
title_fullStr In Vitro Insulin Resistance Model: A Recent Update
title_full_unstemmed In Vitro Insulin Resistance Model: A Recent Update
title_short In Vitro Insulin Resistance Model: A Recent Update
title_sort in vitro insulin resistance model: a recent update
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9876677/
https://www.ncbi.nlm.nih.gov/pubmed/36714242
http://dx.doi.org/10.1155/2023/1964732
work_keys_str_mv AT yudhaniratihd invitroinsulinresistancemodelarecentupdate
AT sariyulia invitroinsulinresistancemodelarecentupdate
AT nugrahaningsihdwiaa invitroinsulinresistancemodelarecentupdate
AT sholikhahetin invitroinsulinresistancemodelarecentupdate
AT rochmantimaftuchah invitroinsulinresistancemodelarecentupdate
AT purbaabdulkr invitroinsulinresistancemodelarecentupdate
AT khotimahhusnul invitroinsulinresistancemodelarecentupdate
AT nugrahennydian invitroinsulinresistancemodelarecentupdate
AT mustofamustofa invitroinsulinresistancemodelarecentupdate