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In Vitro Insulin Resistance Model: A Recent Update
Insulin resistance, which affects insulin-sensitive tissues, including adipose tissues, skeletal muscle, and the liver, is the central pathophysiological mechanism underlying type 2 diabetes progression. Decreased glucose uptake in insulin-sensitive tissues disrupts insulin signaling pathways, parti...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9876677/ https://www.ncbi.nlm.nih.gov/pubmed/36714242 http://dx.doi.org/10.1155/2023/1964732 |
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author | Yudhani, Ratih D. Sari, Yulia Nugrahaningsih, Dwi A. A. Sholikhah, Eti N. Rochmanti, Maftuchah Purba, Abdul K. R. Khotimah, Husnul Nugrahenny, Dian Mustofa, Mustofa |
author_facet | Yudhani, Ratih D. Sari, Yulia Nugrahaningsih, Dwi A. A. Sholikhah, Eti N. Rochmanti, Maftuchah Purba, Abdul K. R. Khotimah, Husnul Nugrahenny, Dian Mustofa, Mustofa |
author_sort | Yudhani, Ratih D. |
collection | PubMed |
description | Insulin resistance, which affects insulin-sensitive tissues, including adipose tissues, skeletal muscle, and the liver, is the central pathophysiological mechanism underlying type 2 diabetes progression. Decreased glucose uptake in insulin-sensitive tissues disrupts insulin signaling pathways, particularly the PI3K/Akt pathway. An in vitro model is appropriate for studying the cellular and molecular mechanisms underlying insulin resistance because it is easy to maintain and the results can be easily reproduced. The application of cell-based models for exploring the pathogenesis of diabetes and insulin resistance as well as for developing drugs for these conditions is well known. However, a comprehensive review of in vitro insulin resistance models is lacking. Therefore, this review was conducted to provide a comprehensive overview and summary of the latest in vitro insulin resistance models, particularly 3T3-L1 (preadipocyte), C2C12 (skeletal muscle), and HepG2 (liver) cell lines induced with palmitic acid, high glucose, or chronic exposure to insulin. |
format | Online Article Text |
id | pubmed-9876677 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-98766772023-01-26 In Vitro Insulin Resistance Model: A Recent Update Yudhani, Ratih D. Sari, Yulia Nugrahaningsih, Dwi A. A. Sholikhah, Eti N. Rochmanti, Maftuchah Purba, Abdul K. R. Khotimah, Husnul Nugrahenny, Dian Mustofa, Mustofa J Obes Review Article Insulin resistance, which affects insulin-sensitive tissues, including adipose tissues, skeletal muscle, and the liver, is the central pathophysiological mechanism underlying type 2 diabetes progression. Decreased glucose uptake in insulin-sensitive tissues disrupts insulin signaling pathways, particularly the PI3K/Akt pathway. An in vitro model is appropriate for studying the cellular and molecular mechanisms underlying insulin resistance because it is easy to maintain and the results can be easily reproduced. The application of cell-based models for exploring the pathogenesis of diabetes and insulin resistance as well as for developing drugs for these conditions is well known. However, a comprehensive review of in vitro insulin resistance models is lacking. Therefore, this review was conducted to provide a comprehensive overview and summary of the latest in vitro insulin resistance models, particularly 3T3-L1 (preadipocyte), C2C12 (skeletal muscle), and HepG2 (liver) cell lines induced with palmitic acid, high glucose, or chronic exposure to insulin. Hindawi 2023-01-18 /pmc/articles/PMC9876677/ /pubmed/36714242 http://dx.doi.org/10.1155/2023/1964732 Text en Copyright © 2023 Ratih D. Yudhani et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Review Article Yudhani, Ratih D. Sari, Yulia Nugrahaningsih, Dwi A. A. Sholikhah, Eti N. Rochmanti, Maftuchah Purba, Abdul K. R. Khotimah, Husnul Nugrahenny, Dian Mustofa, Mustofa In Vitro Insulin Resistance Model: A Recent Update |
title |
In Vitro Insulin Resistance Model: A Recent Update |
title_full |
In Vitro Insulin Resistance Model: A Recent Update |
title_fullStr |
In Vitro Insulin Resistance Model: A Recent Update |
title_full_unstemmed |
In Vitro Insulin Resistance Model: A Recent Update |
title_short |
In Vitro Insulin Resistance Model: A Recent Update |
title_sort | in vitro insulin resistance model: a recent update |
topic | Review Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9876677/ https://www.ncbi.nlm.nih.gov/pubmed/36714242 http://dx.doi.org/10.1155/2023/1964732 |
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