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Evaluation of cellular response to Clostridium difficile toxin-A: a network analysis
AIM: The current study aimed to determine crucial genes targeted by toxin-A through network analysis. BACKGROUND: Clostridium difficile (C difficile) produces toxin-A and toxin-B and is known as a risk factor for hospital infection, especially after broad spectrum antibiotic therapy. Bioinformatics...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Shaheed Beheshti University of Medical Sciences
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9876764/ https://www.ncbi.nlm.nih.gov/pubmed/36762215 http://dx.doi.org/10.22037/ghfbb.v15i4.2634 |
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author | Arjmand, Babak Jahani Sherafat, Somayeh Rezaei Tavirani, Mostafa Hamzeloo Moghadam, Maryam Khodadoost, Mahmood |
author_facet | Arjmand, Babak Jahani Sherafat, Somayeh Rezaei Tavirani, Mostafa Hamzeloo Moghadam, Maryam Khodadoost, Mahmood |
author_sort | Arjmand, Babak |
collection | PubMed |
description | AIM: The current study aimed to determine crucial genes targeted by toxin-A through network analysis. BACKGROUND: Clostridium difficile (C difficile) produces toxin-A and toxin-B and is known as a risk factor for hospital infection, especially after broad spectrum antibiotic therapy. Bioinformatics findings have led to the introduction of a set of genes and biological terms that are targeted by toxin-B in colon epithelia. METHODS: The significant differentially expressed genes (DEGs) of human intestinal Caco-2 cells treated by toxin-A versus control were retrieved from gene expression omnibus (GEO). The queried DEGs were analyzed using by protein-protein interaction (PPI) network analysis through STRING database and Cytoscape software v.3.7.2. RESULTS: Among 157 significant DEGs, JUN, VEGFA, CDKN1A, ATF3, SNAI1, DUSP1, HSPB1, MCL1, KLF4, FOSL1, HSPA1A, and SQSTM1 were determined as hubs and JUN, DUSP1, DUSP5, EZR, MAP1LC3B, and SQSTM1 were highlighted as bottlenecks. CONCLUSION: JUN, DUSP1, and SQSTM1 are possible drug targets to prevent and treat C difficile infection. |
format | Online Article Text |
id | pubmed-9876764 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Shaheed Beheshti University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-98767642023-02-08 Evaluation of cellular response to Clostridium difficile toxin-A: a network analysis Arjmand, Babak Jahani Sherafat, Somayeh Rezaei Tavirani, Mostafa Hamzeloo Moghadam, Maryam Khodadoost, Mahmood Gastroenterol Hepatol Bed Bench Original Article AIM: The current study aimed to determine crucial genes targeted by toxin-A through network analysis. BACKGROUND: Clostridium difficile (C difficile) produces toxin-A and toxin-B and is known as a risk factor for hospital infection, especially after broad spectrum antibiotic therapy. Bioinformatics findings have led to the introduction of a set of genes and biological terms that are targeted by toxin-B in colon epithelia. METHODS: The significant differentially expressed genes (DEGs) of human intestinal Caco-2 cells treated by toxin-A versus control were retrieved from gene expression omnibus (GEO). The queried DEGs were analyzed using by protein-protein interaction (PPI) network analysis through STRING database and Cytoscape software v.3.7.2. RESULTS: Among 157 significant DEGs, JUN, VEGFA, CDKN1A, ATF3, SNAI1, DUSP1, HSPB1, MCL1, KLF4, FOSL1, HSPA1A, and SQSTM1 were determined as hubs and JUN, DUSP1, DUSP5, EZR, MAP1LC3B, and SQSTM1 were highlighted as bottlenecks. CONCLUSION: JUN, DUSP1, and SQSTM1 are possible drug targets to prevent and treat C difficile infection. Shaheed Beheshti University of Medical Sciences 2022 /pmc/articles/PMC9876764/ /pubmed/36762215 http://dx.doi.org/10.22037/ghfbb.v15i4.2634 Text en https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article, distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (https://creativecommons.org/licenses/by-nc/4.0/) which permits others to copy and redistribute the material just in noncommercial usages, provided the original work is properly cited. |
spellingShingle | Original Article Arjmand, Babak Jahani Sherafat, Somayeh Rezaei Tavirani, Mostafa Hamzeloo Moghadam, Maryam Khodadoost, Mahmood Evaluation of cellular response to Clostridium difficile toxin-A: a network analysis |
title | Evaluation of cellular response to Clostridium
difficile toxin-A: a network analysis |
title_full | Evaluation of cellular response to Clostridium
difficile toxin-A: a network analysis |
title_fullStr | Evaluation of cellular response to Clostridium
difficile toxin-A: a network analysis |
title_full_unstemmed | Evaluation of cellular response to Clostridium
difficile toxin-A: a network analysis |
title_short | Evaluation of cellular response to Clostridium
difficile toxin-A: a network analysis |
title_sort | evaluation of cellular response to clostridium
difficile toxin-a: a network analysis |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9876764/ https://www.ncbi.nlm.nih.gov/pubmed/36762215 http://dx.doi.org/10.22037/ghfbb.v15i4.2634 |
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