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Isolation of single cells from human hepatoblastoma tissues for whole-exome sequencing

By combining single-cell processing with whole-exome sequencing, we have developed single-cell whole-exome sequencing to investigate the mechanisms of hepatoblastoma development and to provide potential targets and therapeutic approaches for clinical treatment. In the following protocol, we outline...

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Detalles Bibliográficos
Autores principales: He, Jian, Meng, Mei, Zhou, Xianchao, Gao, Rui, Wang, Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9876968/
https://www.ncbi.nlm.nih.gov/pubmed/36853859
http://dx.doi.org/10.1016/j.xpro.2023.102052
Descripción
Sumario:By combining single-cell processing with whole-exome sequencing, we have developed single-cell whole-exome sequencing to investigate the mechanisms of hepatoblastoma development and to provide potential targets and therapeutic approaches for clinical treatment. In the following protocol, we outline the steps involved in single-cell sorting, whole-genome amplification, amplification uniformity estimation, and whole-exome library construction. In addition to the cells we use, this protocol is also suitable for other cell lines and cell types. For complete details on the use and execution of this protocol, please refer to Jian et al. (2023).