Direct cell extraction of membrane proteins for structure–function analysis

Membrane proteins are the largest group of therapeutic targets in a variety of disease areas and yet, they remain particularly difficult to investigate. We have developed a novel one-step approach for the incorporation of membrane proteins directly from cells into lipid Salipro nanoparticles. Here,...

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Autores principales: Drulyte, Ieva, Gutgsell, Aspen Rene, Lloris-Garcerá, Pilar, Liss, Michael, Geschwindner, Stefan, Radjainia, Mazdak, Frauenfeld, Jens, Löving, Robin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9876986/
https://www.ncbi.nlm.nih.gov/pubmed/36697499
http://dx.doi.org/10.1038/s41598-023-28455-w
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author Drulyte, Ieva
Gutgsell, Aspen Rene
Lloris-Garcerá, Pilar
Liss, Michael
Geschwindner, Stefan
Radjainia, Mazdak
Frauenfeld, Jens
Löving, Robin
author_facet Drulyte, Ieva
Gutgsell, Aspen Rene
Lloris-Garcerá, Pilar
Liss, Michael
Geschwindner, Stefan
Radjainia, Mazdak
Frauenfeld, Jens
Löving, Robin
author_sort Drulyte, Ieva
collection PubMed
description Membrane proteins are the largest group of therapeutic targets in a variety of disease areas and yet, they remain particularly difficult to investigate. We have developed a novel one-step approach for the incorporation of membrane proteins directly from cells into lipid Salipro nanoparticles. Here, with the pannexin1 channel as a case study, we demonstrate the applicability of this method for structure–function analysis using SPR and cryo-EM.
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spelling pubmed-98769862023-01-27 Direct cell extraction of membrane proteins for structure–function analysis Drulyte, Ieva Gutgsell, Aspen Rene Lloris-Garcerá, Pilar Liss, Michael Geschwindner, Stefan Radjainia, Mazdak Frauenfeld, Jens Löving, Robin Sci Rep Article Membrane proteins are the largest group of therapeutic targets in a variety of disease areas and yet, they remain particularly difficult to investigate. We have developed a novel one-step approach for the incorporation of membrane proteins directly from cells into lipid Salipro nanoparticles. Here, with the pannexin1 channel as a case study, we demonstrate the applicability of this method for structure–function analysis using SPR and cryo-EM. Nature Publishing Group UK 2023-01-25 /pmc/articles/PMC9876986/ /pubmed/36697499 http://dx.doi.org/10.1038/s41598-023-28455-w Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Drulyte, Ieva
Gutgsell, Aspen Rene
Lloris-Garcerá, Pilar
Liss, Michael
Geschwindner, Stefan
Radjainia, Mazdak
Frauenfeld, Jens
Löving, Robin
Direct cell extraction of membrane proteins for structure–function analysis
title Direct cell extraction of membrane proteins for structure–function analysis
title_full Direct cell extraction of membrane proteins for structure–function analysis
title_fullStr Direct cell extraction of membrane proteins for structure–function analysis
title_full_unstemmed Direct cell extraction of membrane proteins for structure–function analysis
title_short Direct cell extraction of membrane proteins for structure–function analysis
title_sort direct cell extraction of membrane proteins for structure–function analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9876986/
https://www.ncbi.nlm.nih.gov/pubmed/36697499
http://dx.doi.org/10.1038/s41598-023-28455-w
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