Cargando…

The Pga59 cell wall protein is an amyloid forming protein involved in adhesion and biofilm establishment in the pathogenic yeast Candida albicans

The human commensal fungus Candida albicans can attach to epithelia or indwelling medical devices and form biofilms, that are highly tolerant to antifungal drugs and can evade the immune response. The cell surface protein Pga59 has been shown to influence adhesion and biofilm formation. Here, we pre...

Descripción completa

Detalles Bibliográficos
Autores principales: Mourer, Thierry, El Ghalid, Mennat, Pehau-Arnaudet, Gérard, Kauffmann, Brice, Loquet, Antoine, Brûlé, Sébastien, Cabral, Vitor, d’Enfert, Christophe, Bachellier-Bassi, Sophie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9877000/
https://www.ncbi.nlm.nih.gov/pubmed/36697414
http://dx.doi.org/10.1038/s41522-023-00371-x
_version_ 1784878289720967168
author Mourer, Thierry
El Ghalid, Mennat
Pehau-Arnaudet, Gérard
Kauffmann, Brice
Loquet, Antoine
Brûlé, Sébastien
Cabral, Vitor
d’Enfert, Christophe
Bachellier-Bassi, Sophie
author_facet Mourer, Thierry
El Ghalid, Mennat
Pehau-Arnaudet, Gérard
Kauffmann, Brice
Loquet, Antoine
Brûlé, Sébastien
Cabral, Vitor
d’Enfert, Christophe
Bachellier-Bassi, Sophie
author_sort Mourer, Thierry
collection PubMed
description The human commensal fungus Candida albicans can attach to epithelia or indwelling medical devices and form biofilms, that are highly tolerant to antifungal drugs and can evade the immune response. The cell surface protein Pga59 has been shown to influence adhesion and biofilm formation. Here, we present evidence that Pga59 displays amyloid properties. Using electron microscopy, staining with an amyloid fibre-specific dye and X-ray diffraction experiments, we showed that the predicted amyloid-forming region of Pga59 is sufficient to build up an amyloid fibre in vitro and that recombinant Pga59 can also adopt a cross-β amyloid fibre architecture. Further, mutations impairing Pga59 amyloid assembly led to diminished adhesion to substrates and reduced biofilm production. Immunogold labelling on amyloid structures extracted from C. albicans revealed that Pga59 is used by the fungal cell to assemble amyloids within the cell wall in response to adhesion. Altogether, our results suggest that Pga59 amyloid properties are used by the fungal cell to mediate cell-substrate interactions and biofilm formation.
format Online
Article
Text
id pubmed-9877000
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-98770002023-01-27 The Pga59 cell wall protein is an amyloid forming protein involved in adhesion and biofilm establishment in the pathogenic yeast Candida albicans Mourer, Thierry El Ghalid, Mennat Pehau-Arnaudet, Gérard Kauffmann, Brice Loquet, Antoine Brûlé, Sébastien Cabral, Vitor d’Enfert, Christophe Bachellier-Bassi, Sophie NPJ Biofilms Microbiomes Article The human commensal fungus Candida albicans can attach to epithelia or indwelling medical devices and form biofilms, that are highly tolerant to antifungal drugs and can evade the immune response. The cell surface protein Pga59 has been shown to influence adhesion and biofilm formation. Here, we present evidence that Pga59 displays amyloid properties. Using electron microscopy, staining with an amyloid fibre-specific dye and X-ray diffraction experiments, we showed that the predicted amyloid-forming region of Pga59 is sufficient to build up an amyloid fibre in vitro and that recombinant Pga59 can also adopt a cross-β amyloid fibre architecture. Further, mutations impairing Pga59 amyloid assembly led to diminished adhesion to substrates and reduced biofilm production. Immunogold labelling on amyloid structures extracted from C. albicans revealed that Pga59 is used by the fungal cell to assemble amyloids within the cell wall in response to adhesion. Altogether, our results suggest that Pga59 amyloid properties are used by the fungal cell to mediate cell-substrate interactions and biofilm formation. Nature Publishing Group UK 2023-01-25 /pmc/articles/PMC9877000/ /pubmed/36697414 http://dx.doi.org/10.1038/s41522-023-00371-x Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Mourer, Thierry
El Ghalid, Mennat
Pehau-Arnaudet, Gérard
Kauffmann, Brice
Loquet, Antoine
Brûlé, Sébastien
Cabral, Vitor
d’Enfert, Christophe
Bachellier-Bassi, Sophie
The Pga59 cell wall protein is an amyloid forming protein involved in adhesion and biofilm establishment in the pathogenic yeast Candida albicans
title The Pga59 cell wall protein is an amyloid forming protein involved in adhesion and biofilm establishment in the pathogenic yeast Candida albicans
title_full The Pga59 cell wall protein is an amyloid forming protein involved in adhesion and biofilm establishment in the pathogenic yeast Candida albicans
title_fullStr The Pga59 cell wall protein is an amyloid forming protein involved in adhesion and biofilm establishment in the pathogenic yeast Candida albicans
title_full_unstemmed The Pga59 cell wall protein is an amyloid forming protein involved in adhesion and biofilm establishment in the pathogenic yeast Candida albicans
title_short The Pga59 cell wall protein is an amyloid forming protein involved in adhesion and biofilm establishment in the pathogenic yeast Candida albicans
title_sort pga59 cell wall protein is an amyloid forming protein involved in adhesion and biofilm establishment in the pathogenic yeast candida albicans
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9877000/
https://www.ncbi.nlm.nih.gov/pubmed/36697414
http://dx.doi.org/10.1038/s41522-023-00371-x
work_keys_str_mv AT mourerthierry thepga59cellwallproteinisanamyloidformingproteininvolvedinadhesionandbiofilmestablishmentinthepathogenicyeastcandidaalbicans
AT elghalidmennat thepga59cellwallproteinisanamyloidformingproteininvolvedinadhesionandbiofilmestablishmentinthepathogenicyeastcandidaalbicans
AT pehauarnaudetgerard thepga59cellwallproteinisanamyloidformingproteininvolvedinadhesionandbiofilmestablishmentinthepathogenicyeastcandidaalbicans
AT kauffmannbrice thepga59cellwallproteinisanamyloidformingproteininvolvedinadhesionandbiofilmestablishmentinthepathogenicyeastcandidaalbicans
AT loquetantoine thepga59cellwallproteinisanamyloidformingproteininvolvedinadhesionandbiofilmestablishmentinthepathogenicyeastcandidaalbicans
AT brulesebastien thepga59cellwallproteinisanamyloidformingproteininvolvedinadhesionandbiofilmestablishmentinthepathogenicyeastcandidaalbicans
AT cabralvitor thepga59cellwallproteinisanamyloidformingproteininvolvedinadhesionandbiofilmestablishmentinthepathogenicyeastcandidaalbicans
AT denfertchristophe thepga59cellwallproteinisanamyloidformingproteininvolvedinadhesionandbiofilmestablishmentinthepathogenicyeastcandidaalbicans
AT bachellierbassisophie thepga59cellwallproteinisanamyloidformingproteininvolvedinadhesionandbiofilmestablishmentinthepathogenicyeastcandidaalbicans
AT mourerthierry pga59cellwallproteinisanamyloidformingproteininvolvedinadhesionandbiofilmestablishmentinthepathogenicyeastcandidaalbicans
AT elghalidmennat pga59cellwallproteinisanamyloidformingproteininvolvedinadhesionandbiofilmestablishmentinthepathogenicyeastcandidaalbicans
AT pehauarnaudetgerard pga59cellwallproteinisanamyloidformingproteininvolvedinadhesionandbiofilmestablishmentinthepathogenicyeastcandidaalbicans
AT kauffmannbrice pga59cellwallproteinisanamyloidformingproteininvolvedinadhesionandbiofilmestablishmentinthepathogenicyeastcandidaalbicans
AT loquetantoine pga59cellwallproteinisanamyloidformingproteininvolvedinadhesionandbiofilmestablishmentinthepathogenicyeastcandidaalbicans
AT brulesebastien pga59cellwallproteinisanamyloidformingproteininvolvedinadhesionandbiofilmestablishmentinthepathogenicyeastcandidaalbicans
AT cabralvitor pga59cellwallproteinisanamyloidformingproteininvolvedinadhesionandbiofilmestablishmentinthepathogenicyeastcandidaalbicans
AT denfertchristophe pga59cellwallproteinisanamyloidformingproteininvolvedinadhesionandbiofilmestablishmentinthepathogenicyeastcandidaalbicans
AT bachellierbassisophie pga59cellwallproteinisanamyloidformingproteininvolvedinadhesionandbiofilmestablishmentinthepathogenicyeastcandidaalbicans