Tandem mass tag-based quantitative proteomics analysis reveals the new regulatory mechanism of progranulin in influenza virus infection

Progranulin (PGRN) plays an important role in influenza virus infection. To gain insight into the potential molecular mechanisms by which PGRN regulates influenza viral replication, proteomic analyzes of whole mouse lung tissue from wild-type (WT) versus (vs) PGRN knockout (KO) mice were performed t...

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Autores principales: Li, Haoning, Zhang, Yuying, Li, Chengye, Ning, Peng, Sun, Hailiang, Wei, Fanhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9877624/
https://www.ncbi.nlm.nih.gov/pubmed/36713155
http://dx.doi.org/10.3389/fmicb.2022.1090851
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author Li, Haoning
Zhang, Yuying
Li, Chengye
Ning, Peng
Sun, Hailiang
Wei, Fanhua
author_facet Li, Haoning
Zhang, Yuying
Li, Chengye
Ning, Peng
Sun, Hailiang
Wei, Fanhua
author_sort Li, Haoning
collection PubMed
description Progranulin (PGRN) plays an important role in influenza virus infection. To gain insight into the potential molecular mechanisms by which PGRN regulates influenza viral replication, proteomic analyzes of whole mouse lung tissue from wild-type (WT) versus (vs) PGRN knockout (KO) mice were performed to identify proteins regulated by the absence vs. presence of PGRN. Our results revealed that PGRN regulated the differential expression of ALOX15, CD14, CD5L, and FCER1g, etc., and also affected the lysosomal activity in influenza virus infection. Collectively these findings provide a panoramic view of proteomic changes resulting from loss of PGRN and thereby shedding light on the functions of PGRN in influenza virus infection.
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spelling pubmed-98776242023-01-27 Tandem mass tag-based quantitative proteomics analysis reveals the new regulatory mechanism of progranulin in influenza virus infection Li, Haoning Zhang, Yuying Li, Chengye Ning, Peng Sun, Hailiang Wei, Fanhua Front Microbiol Microbiology Progranulin (PGRN) plays an important role in influenza virus infection. To gain insight into the potential molecular mechanisms by which PGRN regulates influenza viral replication, proteomic analyzes of whole mouse lung tissue from wild-type (WT) versus (vs) PGRN knockout (KO) mice were performed to identify proteins regulated by the absence vs. presence of PGRN. Our results revealed that PGRN regulated the differential expression of ALOX15, CD14, CD5L, and FCER1g, etc., and also affected the lysosomal activity in influenza virus infection. Collectively these findings provide a panoramic view of proteomic changes resulting from loss of PGRN and thereby shedding light on the functions of PGRN in influenza virus infection. Frontiers Media S.A. 2023-01-12 /pmc/articles/PMC9877624/ /pubmed/36713155 http://dx.doi.org/10.3389/fmicb.2022.1090851 Text en Copyright © 2023 Li, Zhang, Li, Ning, Sun and Wei. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Li, Haoning
Zhang, Yuying
Li, Chengye
Ning, Peng
Sun, Hailiang
Wei, Fanhua
Tandem mass tag-based quantitative proteomics analysis reveals the new regulatory mechanism of progranulin in influenza virus infection
title Tandem mass tag-based quantitative proteomics analysis reveals the new regulatory mechanism of progranulin in influenza virus infection
title_full Tandem mass tag-based quantitative proteomics analysis reveals the new regulatory mechanism of progranulin in influenza virus infection
title_fullStr Tandem mass tag-based quantitative proteomics analysis reveals the new regulatory mechanism of progranulin in influenza virus infection
title_full_unstemmed Tandem mass tag-based quantitative proteomics analysis reveals the new regulatory mechanism of progranulin in influenza virus infection
title_short Tandem mass tag-based quantitative proteomics analysis reveals the new regulatory mechanism of progranulin in influenza virus infection
title_sort tandem mass tag-based quantitative proteomics analysis reveals the new regulatory mechanism of progranulin in influenza virus infection
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9877624/
https://www.ncbi.nlm.nih.gov/pubmed/36713155
http://dx.doi.org/10.3389/fmicb.2022.1090851
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