Cargando…

Lactic acid bacteria in cow raw milk for cheese production: Which and how many?

Lactic Acid Bacteria (LAB) exert a fundamental activity in cheese production, as starter LAB in curd acidification, or non-starter LAB (NSLAB) during ripening, in particular in flavor formation. NSLAB originate from the farm and dairy environment, becoming natural contaminants of raw milk where they...

Descripción completa

Detalles Bibliográficos
Autores principales: Bettera, Luca, Levante, Alessia, Bancalari, Elena, Bottari, Benedetta, Gatti, Monica
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9878191/
https://www.ncbi.nlm.nih.gov/pubmed/36713157
http://dx.doi.org/10.3389/fmicb.2022.1092224
_version_ 1784878447590375424
author Bettera, Luca
Levante, Alessia
Bancalari, Elena
Bottari, Benedetta
Gatti, Monica
author_facet Bettera, Luca
Levante, Alessia
Bancalari, Elena
Bottari, Benedetta
Gatti, Monica
author_sort Bettera, Luca
collection PubMed
description Lactic Acid Bacteria (LAB) exert a fundamental activity in cheese production, as starter LAB in curd acidification, or non-starter LAB (NSLAB) during ripening, in particular in flavor formation. NSLAB originate from the farm and dairy environment, becoming natural contaminants of raw milk where they are present in very low concentrations. Afterward, throughout the different cheesemaking processes, they withstand chemical and physical stresses becoming dominant in ripened cheeses. However, despite a great body of knowledge is available in the literature about NSLAB effect on cheese ripening, the investigations regarding their presence and abundance in raw milk are still poor. With the aim to answer the initial question: “which and how many LAB are present in cow raw milk used for cheese production?,” this review has been divided in two main parts. The first one gives an overview of LAB presence in the complex microbiota of raw milk through the meta-analysis of recent taxonomic studies. In the second part, we present a collection of data about LAB quantification in raw milk by culture-dependent analysis, retrieved through a systematic review. Essentially, the revision of data obtained by plate counts on selective agar media showed an average higher concentration of coccoid LAB than lactobacilli, which was found to be consistent with meta-taxonomic analysis. The advantages of the impedometric technique applied to the quantification of LAB in raw milk were also briefly discussed with a focus on the statistical significance of the obtainable data. Furthermore, this approach was also found to be more accurate in highlighting that microorganisms other than LAB are the major component of raw milk. Nevertheless, the variability of the results observed in the studies based on the same counting methodology, highlights that different sampling methods, as well as the “history” of milk before analysis, are variables of great importance that need to be considered in raw milk analysis.
format Online
Article
Text
id pubmed-9878191
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-98781912023-01-27 Lactic acid bacteria in cow raw milk for cheese production: Which and how many? Bettera, Luca Levante, Alessia Bancalari, Elena Bottari, Benedetta Gatti, Monica Front Microbiol Microbiology Lactic Acid Bacteria (LAB) exert a fundamental activity in cheese production, as starter LAB in curd acidification, or non-starter LAB (NSLAB) during ripening, in particular in flavor formation. NSLAB originate from the farm and dairy environment, becoming natural contaminants of raw milk where they are present in very low concentrations. Afterward, throughout the different cheesemaking processes, they withstand chemical and physical stresses becoming dominant in ripened cheeses. However, despite a great body of knowledge is available in the literature about NSLAB effect on cheese ripening, the investigations regarding their presence and abundance in raw milk are still poor. With the aim to answer the initial question: “which and how many LAB are present in cow raw milk used for cheese production?,” this review has been divided in two main parts. The first one gives an overview of LAB presence in the complex microbiota of raw milk through the meta-analysis of recent taxonomic studies. In the second part, we present a collection of data about LAB quantification in raw milk by culture-dependent analysis, retrieved through a systematic review. Essentially, the revision of data obtained by plate counts on selective agar media showed an average higher concentration of coccoid LAB than lactobacilli, which was found to be consistent with meta-taxonomic analysis. The advantages of the impedometric technique applied to the quantification of LAB in raw milk were also briefly discussed with a focus on the statistical significance of the obtainable data. Furthermore, this approach was also found to be more accurate in highlighting that microorganisms other than LAB are the major component of raw milk. Nevertheless, the variability of the results observed in the studies based on the same counting methodology, highlights that different sampling methods, as well as the “history” of milk before analysis, are variables of great importance that need to be considered in raw milk analysis. Frontiers Media S.A. 2023-01-12 /pmc/articles/PMC9878191/ /pubmed/36713157 http://dx.doi.org/10.3389/fmicb.2022.1092224 Text en Copyright © 2023 Bettera, Levante, Bancalari, Bottari and Gatti. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Bettera, Luca
Levante, Alessia
Bancalari, Elena
Bottari, Benedetta
Gatti, Monica
Lactic acid bacteria in cow raw milk for cheese production: Which and how many?
title Lactic acid bacteria in cow raw milk for cheese production: Which and how many?
title_full Lactic acid bacteria in cow raw milk for cheese production: Which and how many?
title_fullStr Lactic acid bacteria in cow raw milk for cheese production: Which and how many?
title_full_unstemmed Lactic acid bacteria in cow raw milk for cheese production: Which and how many?
title_short Lactic acid bacteria in cow raw milk for cheese production: Which and how many?
title_sort lactic acid bacteria in cow raw milk for cheese production: which and how many?
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9878191/
https://www.ncbi.nlm.nih.gov/pubmed/36713157
http://dx.doi.org/10.3389/fmicb.2022.1092224
work_keys_str_mv AT betteraluca lacticacidbacteriaincowrawmilkforcheeseproductionwhichandhowmany
AT levantealessia lacticacidbacteriaincowrawmilkforcheeseproductionwhichandhowmany
AT bancalarielena lacticacidbacteriaincowrawmilkforcheeseproductionwhichandhowmany
AT bottaribenedetta lacticacidbacteriaincowrawmilkforcheeseproductionwhichandhowmany
AT gattimonica lacticacidbacteriaincowrawmilkforcheeseproductionwhichandhowmany