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The probiotic and immunomodulation effects of Limosilactobacillus reuteri RGW1 isolated from calf feces
INTRODUCTION: Limosilactobacillus reuteri is a gut symbiont with multiple remarkable beneficial effects on host health, and members of L. reuteri are valuable probiotic agents. However, L. reuteri showed obvious host specificity. METHODS: In our study, a novel L. reuteri RGW1 was isolated from feces...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9879569/ https://www.ncbi.nlm.nih.gov/pubmed/36710979 http://dx.doi.org/10.3389/fcimb.2022.1086861 |
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author | Huang, Kailang Shi, Weibing Yang, Bin Wang, Jiakun |
author_facet | Huang, Kailang Shi, Weibing Yang, Bin Wang, Jiakun |
author_sort | Huang, Kailang |
collection | PubMed |
description | INTRODUCTION: Limosilactobacillus reuteri is a gut symbiont with multiple remarkable beneficial effects on host health, and members of L. reuteri are valuable probiotic agents. However, L. reuteri showed obvious host specificity. METHODS: In our study, a novel L. reuteri RGW1 was isolated from feces of healthy calves, and its potential as a probiotic candidate were assessed, by combining in vitro, in vivo experiments and genomic analysis. RESULTS AND DISCUSSION: RGW1 was sensitive to all the antibiotics tested, and it did not contain any virulence factor-coding genes. This isolate showed good tolerance to acid (pH 3.0), 0.3% bile salt, and simulated gastric fluid. Moreover, this isolate showed a high hydrophobicity index (73.7 ± 4.6%) and was able to adhere to Caco-2 cells, and antagonize Escherichia coli F5. Treatment of LPS-induced mice with RGW1 elevated TGF-β and IL-10 levels, while RGW1 cell-free supernatant (RCS) decreased TNF-α levels in the sera. Both RGW1 and RCS increased the villus height and villus height/crypt depth ratio of colon. Genomic analysis revealed the mechanism of the probiotic properties described above, and identified the capacity of RGW1 to biosynthesize L-lysine, folate, cobalamin and reuterin de novo. Our study demonstrated the novel bovine origin L. reuteri RGW1 had multiple probiotic characteristics and immunomodulation effects, and provided a deeper understanding of the relationship between these probiotic properties and genetic features. |
format | Online Article Text |
id | pubmed-9879569 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-98795692023-01-27 The probiotic and immunomodulation effects of Limosilactobacillus reuteri RGW1 isolated from calf feces Huang, Kailang Shi, Weibing Yang, Bin Wang, Jiakun Front Cell Infect Microbiol Cellular and Infection Microbiology INTRODUCTION: Limosilactobacillus reuteri is a gut symbiont with multiple remarkable beneficial effects on host health, and members of L. reuteri are valuable probiotic agents. However, L. reuteri showed obvious host specificity. METHODS: In our study, a novel L. reuteri RGW1 was isolated from feces of healthy calves, and its potential as a probiotic candidate were assessed, by combining in vitro, in vivo experiments and genomic analysis. RESULTS AND DISCUSSION: RGW1 was sensitive to all the antibiotics tested, and it did not contain any virulence factor-coding genes. This isolate showed good tolerance to acid (pH 3.0), 0.3% bile salt, and simulated gastric fluid. Moreover, this isolate showed a high hydrophobicity index (73.7 ± 4.6%) and was able to adhere to Caco-2 cells, and antagonize Escherichia coli F5. Treatment of LPS-induced mice with RGW1 elevated TGF-β and IL-10 levels, while RGW1 cell-free supernatant (RCS) decreased TNF-α levels in the sera. Both RGW1 and RCS increased the villus height and villus height/crypt depth ratio of colon. Genomic analysis revealed the mechanism of the probiotic properties described above, and identified the capacity of RGW1 to biosynthesize L-lysine, folate, cobalamin and reuterin de novo. Our study demonstrated the novel bovine origin L. reuteri RGW1 had multiple probiotic characteristics and immunomodulation effects, and provided a deeper understanding of the relationship between these probiotic properties and genetic features. Frontiers Media S.A. 2023-01-12 /pmc/articles/PMC9879569/ /pubmed/36710979 http://dx.doi.org/10.3389/fcimb.2022.1086861 Text en Copyright © 2023 Huang, Shi, Yang and Wang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cellular and Infection Microbiology Huang, Kailang Shi, Weibing Yang, Bin Wang, Jiakun The probiotic and immunomodulation effects of Limosilactobacillus reuteri RGW1 isolated from calf feces |
title | The probiotic and immunomodulation effects of Limosilactobacillus reuteri RGW1 isolated from calf feces |
title_full | The probiotic and immunomodulation effects of Limosilactobacillus reuteri RGW1 isolated from calf feces |
title_fullStr | The probiotic and immunomodulation effects of Limosilactobacillus reuteri RGW1 isolated from calf feces |
title_full_unstemmed | The probiotic and immunomodulation effects of Limosilactobacillus reuteri RGW1 isolated from calf feces |
title_short | The probiotic and immunomodulation effects of Limosilactobacillus reuteri RGW1 isolated from calf feces |
title_sort | probiotic and immunomodulation effects of limosilactobacillus reuteri rgw1 isolated from calf feces |
topic | Cellular and Infection Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9879569/ https://www.ncbi.nlm.nih.gov/pubmed/36710979 http://dx.doi.org/10.3389/fcimb.2022.1086861 |
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