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Genomic characterization and assessment of pathogenic potential of Legionella spp. isolates from environmental monitoring

Several species in the genus Legionella are known to cause an acute pneumonia when the aerosols containing the bacteria from man-made water systems are inhaled. The disease is usually caused by Legionella pneumophila, but other species have been implicated in the infection. The disease is frequently...

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Autores principales: Svetlicic, Ema, Jaén-Luchoro, Daniel, Klobucar, Roberta Sauerborn, Jers, Carsten, Kazazic, Snjezana, Franjevic, Damjan, Klobucar, Goran, Shelton, Brian G., Mijakovic, Ivan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9879626/
https://www.ncbi.nlm.nih.gov/pubmed/36713227
http://dx.doi.org/10.3389/fmicb.2022.1091964
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author Svetlicic, Ema
Jaén-Luchoro, Daniel
Klobucar, Roberta Sauerborn
Jers, Carsten
Kazazic, Snjezana
Franjevic, Damjan
Klobucar, Goran
Shelton, Brian G.
Mijakovic, Ivan
author_facet Svetlicic, Ema
Jaén-Luchoro, Daniel
Klobucar, Roberta Sauerborn
Jers, Carsten
Kazazic, Snjezana
Franjevic, Damjan
Klobucar, Goran
Shelton, Brian G.
Mijakovic, Ivan
author_sort Svetlicic, Ema
collection PubMed
description Several species in the genus Legionella are known to cause an acute pneumonia when the aerosols containing the bacteria from man-made water systems are inhaled. The disease is usually caused by Legionella pneumophila, but other species have been implicated in the infection. The disease is frequently manifested as an outbreak, which means several people are affected when exposed to the common source of Legionella contamination. Therefor environmental surveillance which includes isolation and identification of Legionella is performed routinely. However, usually no molecular or genome-based methods are employed in further characterization of the isolates during routine environmental monitoring. During several years of such monitoring, isolates from different geographical locations were collected and 39 of them were sequenced by hybrid de novo approach utilizing short and long sequencing reads. In addition, the isolates were typed by standard culture and MALDI-TOF method. The sequencing reads were assembled and annotated to produce high-quality genomes. By employing discriminatory genome typing, four potential new species in the Legionella genus were identified, which are yet to be biochemically and morphologically characterized. Moreover, functional annotations concerning virulence and antimicrobial resistance were performed on the sequenced genomes. The study contributes to the knowledge on little-known non-pneumophila species present in man-made water systems and establishes support for future genetic relatedness studies as well as understanding of their pathogenic potential.
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spelling pubmed-98796262023-01-27 Genomic characterization and assessment of pathogenic potential of Legionella spp. isolates from environmental monitoring Svetlicic, Ema Jaén-Luchoro, Daniel Klobucar, Roberta Sauerborn Jers, Carsten Kazazic, Snjezana Franjevic, Damjan Klobucar, Goran Shelton, Brian G. Mijakovic, Ivan Front Microbiol Microbiology Several species in the genus Legionella are known to cause an acute pneumonia when the aerosols containing the bacteria from man-made water systems are inhaled. The disease is usually caused by Legionella pneumophila, but other species have been implicated in the infection. The disease is frequently manifested as an outbreak, which means several people are affected when exposed to the common source of Legionella contamination. Therefor environmental surveillance which includes isolation and identification of Legionella is performed routinely. However, usually no molecular or genome-based methods are employed in further characterization of the isolates during routine environmental monitoring. During several years of such monitoring, isolates from different geographical locations were collected and 39 of them were sequenced by hybrid de novo approach utilizing short and long sequencing reads. In addition, the isolates were typed by standard culture and MALDI-TOF method. The sequencing reads were assembled and annotated to produce high-quality genomes. By employing discriminatory genome typing, four potential new species in the Legionella genus were identified, which are yet to be biochemically and morphologically characterized. Moreover, functional annotations concerning virulence and antimicrobial resistance were performed on the sequenced genomes. The study contributes to the knowledge on little-known non-pneumophila species present in man-made water systems and establishes support for future genetic relatedness studies as well as understanding of their pathogenic potential. Frontiers Media S.A. 2023-01-12 /pmc/articles/PMC9879626/ /pubmed/36713227 http://dx.doi.org/10.3389/fmicb.2022.1091964 Text en Copyright © 2023 Svetlicic, Jaén-Luchoro, Klobucar, Jers, Kazazic, Franjevic, Klobucar, Shelton and Mijakovic. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Svetlicic, Ema
Jaén-Luchoro, Daniel
Klobucar, Roberta Sauerborn
Jers, Carsten
Kazazic, Snjezana
Franjevic, Damjan
Klobucar, Goran
Shelton, Brian G.
Mijakovic, Ivan
Genomic characterization and assessment of pathogenic potential of Legionella spp. isolates from environmental monitoring
title Genomic characterization and assessment of pathogenic potential of Legionella spp. isolates from environmental monitoring
title_full Genomic characterization and assessment of pathogenic potential of Legionella spp. isolates from environmental monitoring
title_fullStr Genomic characterization and assessment of pathogenic potential of Legionella spp. isolates from environmental monitoring
title_full_unstemmed Genomic characterization and assessment of pathogenic potential of Legionella spp. isolates from environmental monitoring
title_short Genomic characterization and assessment of pathogenic potential of Legionella spp. isolates from environmental monitoring
title_sort genomic characterization and assessment of pathogenic potential of legionella spp. isolates from environmental monitoring
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9879626/
https://www.ncbi.nlm.nih.gov/pubmed/36713227
http://dx.doi.org/10.3389/fmicb.2022.1091964
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