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USP49 deubiquitinase regulates the mitotic spindle checkpoint and prevents aneuploidy
The spindle assembly checkpoint (SAC) is an essential mechanism that ensures the accurate chromosome segregation during mitosis, thus preventing genomic instability. Deubiquitinases have emerged as key regulators of the SAC, mainly by determining the fate of proteins during cell cycle progression. H...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9879932/ https://www.ncbi.nlm.nih.gov/pubmed/36702832 http://dx.doi.org/10.1038/s41419-023-05600-x |
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author | Campos-Iglesias, Diana Fraile, Julia M. Bretones, Gabriel Montero, Alejandro A. Bonzon-Kulichenko, Elena Vázquez, Jesús López-Otín, Carlos Freije, José M. P. |
author_facet | Campos-Iglesias, Diana Fraile, Julia M. Bretones, Gabriel Montero, Alejandro A. Bonzon-Kulichenko, Elena Vázquez, Jesús López-Otín, Carlos Freije, José M. P. |
author_sort | Campos-Iglesias, Diana |
collection | PubMed |
description | The spindle assembly checkpoint (SAC) is an essential mechanism that ensures the accurate chromosome segregation during mitosis, thus preventing genomic instability. Deubiquitinases have emerged as key regulators of the SAC, mainly by determining the fate of proteins during cell cycle progression. Here, we identify USP49 deubiquitinase as a novel regulator of the spindle checkpoint. We show that loss of USP49 in different cancer cell lines impairs proliferation and increases aneuploidy. In addition, USP49-depleted cells overcome the arrest induced by the SAC in the presence of nocodazole. Finally, we report new binding partners of USP49, including ribophorin 1, USP44, and different centrins. |
format | Online Article Text |
id | pubmed-9879932 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-98799322023-01-28 USP49 deubiquitinase regulates the mitotic spindle checkpoint and prevents aneuploidy Campos-Iglesias, Diana Fraile, Julia M. Bretones, Gabriel Montero, Alejandro A. Bonzon-Kulichenko, Elena Vázquez, Jesús López-Otín, Carlos Freije, José M. P. Cell Death Dis Article The spindle assembly checkpoint (SAC) is an essential mechanism that ensures the accurate chromosome segregation during mitosis, thus preventing genomic instability. Deubiquitinases have emerged as key regulators of the SAC, mainly by determining the fate of proteins during cell cycle progression. Here, we identify USP49 deubiquitinase as a novel regulator of the spindle checkpoint. We show that loss of USP49 in different cancer cell lines impairs proliferation and increases aneuploidy. In addition, USP49-depleted cells overcome the arrest induced by the SAC in the presence of nocodazole. Finally, we report new binding partners of USP49, including ribophorin 1, USP44, and different centrins. Nature Publishing Group UK 2023-01-26 /pmc/articles/PMC9879932/ /pubmed/36702832 http://dx.doi.org/10.1038/s41419-023-05600-x Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Campos-Iglesias, Diana Fraile, Julia M. Bretones, Gabriel Montero, Alejandro A. Bonzon-Kulichenko, Elena Vázquez, Jesús López-Otín, Carlos Freije, José M. P. USP49 deubiquitinase regulates the mitotic spindle checkpoint and prevents aneuploidy |
title | USP49 deubiquitinase regulates the mitotic spindle checkpoint and prevents aneuploidy |
title_full | USP49 deubiquitinase regulates the mitotic spindle checkpoint and prevents aneuploidy |
title_fullStr | USP49 deubiquitinase regulates the mitotic spindle checkpoint and prevents aneuploidy |
title_full_unstemmed | USP49 deubiquitinase regulates the mitotic spindle checkpoint and prevents aneuploidy |
title_short | USP49 deubiquitinase regulates the mitotic spindle checkpoint and prevents aneuploidy |
title_sort | usp49 deubiquitinase regulates the mitotic spindle checkpoint and prevents aneuploidy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9879932/ https://www.ncbi.nlm.nih.gov/pubmed/36702832 http://dx.doi.org/10.1038/s41419-023-05600-x |
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