Pigs with an INS point mutation derived from zygotes electroporated with CRISPR/Cas9 and ssODN

Just one amino acid at the carboxy-terminus of the B chain distinguishes human insulin from porcine insulin. By introducing a precise point mutation into the porcine insulin (INS) gene, we were able to generate genetically modified pigs that secreted human insulin; these pigs may be suitable donors...

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Detalles Bibliográficos
Autores principales: Tanihara, Fuminori, Hirata, Maki, Namula, Zhao, Do, Lanh Thi Kim, Yoshimura, Naoaki, Lin, Qingyi, Takebayashi, Koki, Sakuma, Tetsushi, Yamamoto, Takashi, Otoi, Takeshige
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Cell and Developmental Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9880039/
https://www.ncbi.nlm.nih.gov/pubmed/36711037
http://dx.doi.org/10.3389/fcell.2023.884340

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9880039/
https://www.ncbi.nlm.nih.gov/pubmed/36711037
http://dx.doi.org/10.3389/fcell.2023.884340

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