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Bacteriophage efficacy in controlling swine enteric colibacillosis pathogens: An in vitro study
BACKGROUND AND AIM: Swine enteric colibacillosis caused by Escherichia coli is a major problem in the swine industry, causing diarrhea among swine and resulting in substantial financial losses. However, efforts to counter this disease are impeded by the increase in antimicrobial resistance (AMR) wor...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Veterinary World
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9880851/ https://www.ncbi.nlm.nih.gov/pubmed/36718320 http://dx.doi.org/10.14202/vetworld.2022.2822-2829 |
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author | Songphasuk, Techaphon Imklin, Napakhwan Sriprasong, Pattaraporn Woonwong, Yonlayong Nasanit, Rujikan Sajapitak, Somchai |
author_facet | Songphasuk, Techaphon Imklin, Napakhwan Sriprasong, Pattaraporn Woonwong, Yonlayong Nasanit, Rujikan Sajapitak, Somchai |
author_sort | Songphasuk, Techaphon |
collection | PubMed |
description | BACKGROUND AND AIM: Swine enteric colibacillosis caused by Escherichia coli is a major problem in the swine industry, causing diarrhea among swine and resulting in substantial financial losses. However, efforts to counter this disease are impeded by the increase in antimicrobial resistance (AMR) worldwide, so intensive research is being conducted to identify alternative treatments. This study isolated, characterized, and evaluated the efficacy of bacteriophages to control pathogens causative of swine enteric colibacillosis. MATERIALS AND METHODS: Five sewage samples were collected from different areas of a swine farm in Suphanburi province, Thailand and the bacteriophages were enriched and isolated, followed by purification by the agar overlay method using E. coli RENR as the host strain. The selected phages were characterized by evaluating their morphology, while their specificity was verified by the host range test. The efficiency of plating and multiplicity of infection (MOI) were also determined. RESULTS: Four selected phages, namely, vB_Eco-RPNE4i3, vB_Eco-RPNE6i4, vB_Eco-RPNE7i1, and vB_Eco-RPNE8i3, demonstrated different patterns of host range and phage efficiency. They significantly decreased E. coli concentration at the tested MOIs (0.01–100) from 1 h onward. However, bacterial regrowth was observed in all phage treatments. CONCLUSION: This study shows the potential of using phages as an alternative treatment for swine enteric colibacillosis. The obtained results demonstrated that the selected phages had a therapeutic effect against pathogens causative of swine enteric colibacillosis. Therefore, phages could be applied as an alternative treatment to control specific bacterial strains and reduce AMR arising from the overuse of antibiotics. |
format | Online Article Text |
id | pubmed-9880851 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Veterinary World |
record_format | MEDLINE/PubMed |
spelling | pubmed-98808512023-01-29 Bacteriophage efficacy in controlling swine enteric colibacillosis pathogens: An in vitro study Songphasuk, Techaphon Imklin, Napakhwan Sriprasong, Pattaraporn Woonwong, Yonlayong Nasanit, Rujikan Sajapitak, Somchai Vet World Research Article BACKGROUND AND AIM: Swine enteric colibacillosis caused by Escherichia coli is a major problem in the swine industry, causing diarrhea among swine and resulting in substantial financial losses. However, efforts to counter this disease are impeded by the increase in antimicrobial resistance (AMR) worldwide, so intensive research is being conducted to identify alternative treatments. This study isolated, characterized, and evaluated the efficacy of bacteriophages to control pathogens causative of swine enteric colibacillosis. MATERIALS AND METHODS: Five sewage samples were collected from different areas of a swine farm in Suphanburi province, Thailand and the bacteriophages were enriched and isolated, followed by purification by the agar overlay method using E. coli RENR as the host strain. The selected phages were characterized by evaluating their morphology, while their specificity was verified by the host range test. The efficiency of plating and multiplicity of infection (MOI) were also determined. RESULTS: Four selected phages, namely, vB_Eco-RPNE4i3, vB_Eco-RPNE6i4, vB_Eco-RPNE7i1, and vB_Eco-RPNE8i3, demonstrated different patterns of host range and phage efficiency. They significantly decreased E. coli concentration at the tested MOIs (0.01–100) from 1 h onward. However, bacterial regrowth was observed in all phage treatments. CONCLUSION: This study shows the potential of using phages as an alternative treatment for swine enteric colibacillosis. The obtained results demonstrated that the selected phages had a therapeutic effect against pathogens causative of swine enteric colibacillosis. Therefore, phages could be applied as an alternative treatment to control specific bacterial strains and reduce AMR arising from the overuse of antibiotics. Veterinary World 2022-12 2022-12-12 /pmc/articles/PMC9880851/ /pubmed/36718320 http://dx.doi.org/10.14202/vetworld.2022.2822-2829 Text en Copyright: © Songphasuk, et al. https://creativecommons.org/licenses/by/4.0/Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Songphasuk, Techaphon Imklin, Napakhwan Sriprasong, Pattaraporn Woonwong, Yonlayong Nasanit, Rujikan Sajapitak, Somchai Bacteriophage efficacy in controlling swine enteric colibacillosis pathogens: An in vitro study |
title | Bacteriophage efficacy in controlling swine enteric colibacillosis pathogens: An in vitro study |
title_full | Bacteriophage efficacy in controlling swine enteric colibacillosis pathogens: An in vitro study |
title_fullStr | Bacteriophage efficacy in controlling swine enteric colibacillosis pathogens: An in vitro study |
title_full_unstemmed | Bacteriophage efficacy in controlling swine enteric colibacillosis pathogens: An in vitro study |
title_short | Bacteriophage efficacy in controlling swine enteric colibacillosis pathogens: An in vitro study |
title_sort | bacteriophage efficacy in controlling swine enteric colibacillosis pathogens: an in vitro study |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9880851/ https://www.ncbi.nlm.nih.gov/pubmed/36718320 http://dx.doi.org/10.14202/vetworld.2022.2822-2829 |
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