Cargando…

Identification of Age-associated Proteins and Functional Alterations in Human Retinal Pigment Epithelium

Retinal pigment epithelium (RPE) has essential functions, such as nourishing and supporting the neural retina, and is of vital importance in the pathogenesis of age-related retinal degeneration. However, the exact molecular changes of RPE during aging remain poorly understood. Here, we isolated huma...

Descripción completa

Detalles Bibliográficos
Autores principales: Jin, Xiuxiu, Liu, Jingyang, Wang, Weiping, Li, Jiangfeng, Liu, Guangming, Qiu, Ruiqi, Yang, Mingzhu, Liu, Meng, Yang, Lin, Du, Xiaofeng, Lei, Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9880895/
https://www.ncbi.nlm.nih.gov/pubmed/35752290
http://dx.doi.org/10.1016/j.gpb.2022.06.001
_version_ 1784878993379426304
author Jin, Xiuxiu
Liu, Jingyang
Wang, Weiping
Li, Jiangfeng
Liu, Guangming
Qiu, Ruiqi
Yang, Mingzhu
Liu, Meng
Yang, Lin
Du, Xiaofeng
Lei, Bo
author_facet Jin, Xiuxiu
Liu, Jingyang
Wang, Weiping
Li, Jiangfeng
Liu, Guangming
Qiu, Ruiqi
Yang, Mingzhu
Liu, Meng
Yang, Lin
Du, Xiaofeng
Lei, Bo
author_sort Jin, Xiuxiu
collection PubMed
description Retinal pigment epithelium (RPE) has essential functions, such as nourishing and supporting the neural retina, and is of vital importance in the pathogenesis of age-related retinal degeneration. However, the exact molecular changes of RPE during aging remain poorly understood. Here, we isolated human primary RPE (hRPE) cells from 18 eye donors distributed over a wide age range (10–67 years old). A quantitative proteomic analysis was performed to analyze changes in their intracellular and secreted proteins. Age-group related subtypes and age-associated proteins were revealed and potential age-associated mechanisms were validated in ARPE-19 and hRPE cells. The results of proteomic data analysis and verifications suggest that RNF123- and RNF149-related protein ubiquitination plays an important role in protecting hRPE cells from oxidative damage during aging. In older hRPE cells, apoptotic signaling-related pathways were up-regulated, and endoplasmic reticulum organization was down-regulated both in the intracellular and secreted proteomes. Our work paints a detailed molecular picture of hRPE cells during the aging process and provides new insights into the molecular characteristics of RPE during aging and under other related clinical retinal conditions.
format Online
Article
Text
id pubmed-9880895
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-98808952023-01-28 Identification of Age-associated Proteins and Functional Alterations in Human Retinal Pigment Epithelium Jin, Xiuxiu Liu, Jingyang Wang, Weiping Li, Jiangfeng Liu, Guangming Qiu, Ruiqi Yang, Mingzhu Liu, Meng Yang, Lin Du, Xiaofeng Lei, Bo Genomics Proteomics Bioinformatics Original Research Retinal pigment epithelium (RPE) has essential functions, such as nourishing and supporting the neural retina, and is of vital importance in the pathogenesis of age-related retinal degeneration. However, the exact molecular changes of RPE during aging remain poorly understood. Here, we isolated human primary RPE (hRPE) cells from 18 eye donors distributed over a wide age range (10–67 years old). A quantitative proteomic analysis was performed to analyze changes in their intracellular and secreted proteins. Age-group related subtypes and age-associated proteins were revealed and potential age-associated mechanisms were validated in ARPE-19 and hRPE cells. The results of proteomic data analysis and verifications suggest that RNF123- and RNF149-related protein ubiquitination plays an important role in protecting hRPE cells from oxidative damage during aging. In older hRPE cells, apoptotic signaling-related pathways were up-regulated, and endoplasmic reticulum organization was down-regulated both in the intracellular and secreted proteomes. Our work paints a detailed molecular picture of hRPE cells during the aging process and provides new insights into the molecular characteristics of RPE during aging and under other related clinical retinal conditions. Elsevier 2022-08 2022-06-23 /pmc/articles/PMC9880895/ /pubmed/35752290 http://dx.doi.org/10.1016/j.gpb.2022.06.001 Text en © 2022 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Original Research
Jin, Xiuxiu
Liu, Jingyang
Wang, Weiping
Li, Jiangfeng
Liu, Guangming
Qiu, Ruiqi
Yang, Mingzhu
Liu, Meng
Yang, Lin
Du, Xiaofeng
Lei, Bo
Identification of Age-associated Proteins and Functional Alterations in Human Retinal Pigment Epithelium
title Identification of Age-associated Proteins and Functional Alterations in Human Retinal Pigment Epithelium
title_full Identification of Age-associated Proteins and Functional Alterations in Human Retinal Pigment Epithelium
title_fullStr Identification of Age-associated Proteins and Functional Alterations in Human Retinal Pigment Epithelium
title_full_unstemmed Identification of Age-associated Proteins and Functional Alterations in Human Retinal Pigment Epithelium
title_short Identification of Age-associated Proteins and Functional Alterations in Human Retinal Pigment Epithelium
title_sort identification of age-associated proteins and functional alterations in human retinal pigment epithelium
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9880895/
https://www.ncbi.nlm.nih.gov/pubmed/35752290
http://dx.doi.org/10.1016/j.gpb.2022.06.001
work_keys_str_mv AT jinxiuxiu identificationofageassociatedproteinsandfunctionalalterationsinhumanretinalpigmentepithelium
AT liujingyang identificationofageassociatedproteinsandfunctionalalterationsinhumanretinalpigmentepithelium
AT wangweiping identificationofageassociatedproteinsandfunctionalalterationsinhumanretinalpigmentepithelium
AT lijiangfeng identificationofageassociatedproteinsandfunctionalalterationsinhumanretinalpigmentepithelium
AT liuguangming identificationofageassociatedproteinsandfunctionalalterationsinhumanretinalpigmentepithelium
AT qiuruiqi identificationofageassociatedproteinsandfunctionalalterationsinhumanretinalpigmentepithelium
AT yangmingzhu identificationofageassociatedproteinsandfunctionalalterationsinhumanretinalpigmentepithelium
AT liumeng identificationofageassociatedproteinsandfunctionalalterationsinhumanretinalpigmentepithelium
AT yanglin identificationofageassociatedproteinsandfunctionalalterationsinhumanretinalpigmentepithelium
AT duxiaofeng identificationofageassociatedproteinsandfunctionalalterationsinhumanretinalpigmentepithelium
AT leibo identificationofageassociatedproteinsandfunctionalalterationsinhumanretinalpigmentepithelium