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Structural Characterization of Nanoparticle-Supported Lipid Bilayer Arrays by Grazing Incidence X-ray and Neutron Scattering
[Image: see text] Arrays of nanoparticle-supported lipid bilayers (nanoSLB) are lipid-coated nanopatterned interfaces that provide a platform to study curved model biological membranes using surface-sensitive techniques. We combined scattering techniques with direct imaging, to gain access to sub-na...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9880997/ https://www.ncbi.nlm.nih.gov/pubmed/36625710 http://dx.doi.org/10.1021/acsami.2c18956 |
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author | Paracini, Nicolò Gutfreund, Philipp Welbourn, Rebecca Gonzalez-Martinez, Juan Francisco Zhu, Kexin Miao, Yansong Yepuri, Nageshwar Darwish, Tamim A. Garvey, Christopher Waldie, Sarah Larsson, Johan Wolff, Max Cárdenas, Marité |
author_facet | Paracini, Nicolò Gutfreund, Philipp Welbourn, Rebecca Gonzalez-Martinez, Juan Francisco Zhu, Kexin Miao, Yansong Yepuri, Nageshwar Darwish, Tamim A. Garvey, Christopher Waldie, Sarah Larsson, Johan Wolff, Max Cárdenas, Marité |
author_sort | Paracini, Nicolò |
collection | PubMed |
description | [Image: see text] Arrays of nanoparticle-supported lipid bilayers (nanoSLB) are lipid-coated nanopatterned interfaces that provide a platform to study curved model biological membranes using surface-sensitive techniques. We combined scattering techniques with direct imaging, to gain access to sub-nanometer scale structural information on stable nanoparticle monolayers assembled on silicon crystals in a noncovalent manner using a Langmuir–Schaefer deposition. The structure of supported lipid bilayers formed on the nanoparticle arrays via vesicle fusion was investigated using a combination of grazing incidence X-ray and neutron scattering techniques complemented by fluorescence microscopy imaging. Ordered nanoparticle assemblies were shown to be suitable and stable substrates for the formation of curved and fluid lipid bilayers that retained lateral mobility, as shown by fluorescence recovery after photobleaching and quartz crystal microbalance measurements. Neutron reflectometry revealed the formation of high-coverage lipid bilayers around the spherical particles together with a flat lipid bilayer on the substrate below the nanoparticles. The presence of coexisting flat and curved supported lipid bilayers on the same substrate, combined with the sub-nanometer accuracy and isotopic sensitivity of grazing incidence neutron scattering, provides a promising novel approach to investigate curvature-dependent membrane phenomena on supported lipid bilayers. |
format | Online Article Text |
id | pubmed-9880997 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-98809972023-01-28 Structural Characterization of Nanoparticle-Supported Lipid Bilayer Arrays by Grazing Incidence X-ray and Neutron Scattering Paracini, Nicolò Gutfreund, Philipp Welbourn, Rebecca Gonzalez-Martinez, Juan Francisco Zhu, Kexin Miao, Yansong Yepuri, Nageshwar Darwish, Tamim A. Garvey, Christopher Waldie, Sarah Larsson, Johan Wolff, Max Cárdenas, Marité ACS Appl Mater Interfaces [Image: see text] Arrays of nanoparticle-supported lipid bilayers (nanoSLB) are lipid-coated nanopatterned interfaces that provide a platform to study curved model biological membranes using surface-sensitive techniques. We combined scattering techniques with direct imaging, to gain access to sub-nanometer scale structural information on stable nanoparticle monolayers assembled on silicon crystals in a noncovalent manner using a Langmuir–Schaefer deposition. The structure of supported lipid bilayers formed on the nanoparticle arrays via vesicle fusion was investigated using a combination of grazing incidence X-ray and neutron scattering techniques complemented by fluorescence microscopy imaging. Ordered nanoparticle assemblies were shown to be suitable and stable substrates for the formation of curved and fluid lipid bilayers that retained lateral mobility, as shown by fluorescence recovery after photobleaching and quartz crystal microbalance measurements. Neutron reflectometry revealed the formation of high-coverage lipid bilayers around the spherical particles together with a flat lipid bilayer on the substrate below the nanoparticles. The presence of coexisting flat and curved supported lipid bilayers on the same substrate, combined with the sub-nanometer accuracy and isotopic sensitivity of grazing incidence neutron scattering, provides a promising novel approach to investigate curvature-dependent membrane phenomena on supported lipid bilayers. American Chemical Society 2023-01-10 /pmc/articles/PMC9880997/ /pubmed/36625710 http://dx.doi.org/10.1021/acsami.2c18956 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Paracini, Nicolò Gutfreund, Philipp Welbourn, Rebecca Gonzalez-Martinez, Juan Francisco Zhu, Kexin Miao, Yansong Yepuri, Nageshwar Darwish, Tamim A. Garvey, Christopher Waldie, Sarah Larsson, Johan Wolff, Max Cárdenas, Marité Structural Characterization of Nanoparticle-Supported Lipid Bilayer Arrays by Grazing Incidence X-ray and Neutron Scattering |
title | Structural Characterization
of Nanoparticle-Supported
Lipid Bilayer Arrays by Grazing Incidence X-ray and Neutron
Scattering |
title_full | Structural Characterization
of Nanoparticle-Supported
Lipid Bilayer Arrays by Grazing Incidence X-ray and Neutron
Scattering |
title_fullStr | Structural Characterization
of Nanoparticle-Supported
Lipid Bilayer Arrays by Grazing Incidence X-ray and Neutron
Scattering |
title_full_unstemmed | Structural Characterization
of Nanoparticle-Supported
Lipid Bilayer Arrays by Grazing Incidence X-ray and Neutron
Scattering |
title_short | Structural Characterization
of Nanoparticle-Supported
Lipid Bilayer Arrays by Grazing Incidence X-ray and Neutron
Scattering |
title_sort | structural characterization
of nanoparticle-supported
lipid bilayer arrays by grazing incidence x-ray and neutron
scattering |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9880997/ https://www.ncbi.nlm.nih.gov/pubmed/36625710 http://dx.doi.org/10.1021/acsami.2c18956 |
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