Depletion of polyfunctional CD26(high)CD8(+) T cells repertoire in chronic lymphocytic leukemia

BACKGROUND: CD8(+) T cells play an essential role against tumors but the role of human CD8(+)CD26(+) T cell subset against tumors, in particular, haematological cancers such as chronic lymphocytic leukemia (CLL) remains unknown. Although CD4(+)CD26(high) T cells are considered for adoptive cancer immunotherapy, the role of CD8(+)CD26(+) T cells is ill-defined. Therefore, further studies are required to better determine the role of CD8(+)CD26(+) T cells in solid tumors and haematological cancers. METHODS: We studied 55 CLL and 44 age-sex-matched healthy controls (HCs). The expression of CD26 on different T cell subsets (e.g. naïve, memory, effector, and etc.) was analyzed. Also, functional properties of CD8(+)CD26(+) and CD8(+)CD26(−) T cells were evaluated. Finally, the plasma cytokine/chemokine and Galectin-9 (Gal-9) levels were examined. RESULTS: CD26 expression identifies three CD8(+) T cell subsets with distinct immunological properties. While CD26(neg)CD8(+) T cells are mainly transitional, effector memory and effectors, CD26(low)CD8(+) T cells are mainly naïve, stem cell, and central memory but CD26(high) T cells are differentiated to transitional and effector memory. CD26(+)CD8(+) T cells are significantly reduced in CLL patients versus HCs. CD26(high) cells are enriched with Mucosal Associated Invariant T (MAIT) cells co-expressing CD161TVα7.2 and IL-18Rα. Also, CD26(high) cells have a rich chemokine receptor profile (e.g. CCR5 and CCR6), profound cytokine (TNF-α, IFN-γ, and IL-2), and cytolytic molecules (Granzyme B, K, and perforin) expression upon stimulation. CD26(high) and CD26(low) T cells exhibit significantly lower frequencies of CD160, 2B4, TIGIT, ICOS, CD39, and PD-1 but higher levels of CD27, CD28, and CD73 versus CD26(neg) cells. To understand the mechanism linked to CD26(high) depletion, we found that malignant B cells by shedding Galectin-9 (Gal-9) contribute to the elevation of plasma Gal-9 in CLL patients. In turn, Gal-9 and the inflammatory milieu (IL-18, IL-12, and IL-15) in CLL patients contribute to increased apoptosis of CD26(high) T cells. CONCLUSIONS: Our results demonstrate that CD26(+) T cells possess a natural polyfunctionality to traffic and exhibit effector functions and resist exhaustion. Therefore, they can be proposed for adoptive cancer immunotherapy. Finally, neutralizing and/or inhibiting Gal-9 may preserve CD26(high)CD8(+) T cells in CLL. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40164-023-00375-5.