Protocol for absolute quantification of proteins in Gram-negative bacteria based on QconCAT-based labeled peptides

Mass-spectrometry-based absolute protein quantification uses labeled quantification concatamer (QconCAT) as internal standards (ISs). To calculate the amount of protein(s), the ion intensity ratio between the analyte and its cognate IS is compared in each biological sample. The present protocol desc...

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Detalles Bibliográficos
Autores principales: Gurdo, Nicolás, Taylor Parkins, Shannara Kayleigh, Fricano, Martina, Wulff, Tune, Nielsen, Lars Keld, Nikel, Pablo Iván
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9881405/
https://www.ncbi.nlm.nih.gov/pubmed/36853682
http://dx.doi.org/10.1016/j.xpro.2023.102060

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