Generation of two induced pluripotent stem cell lines from dilated cardiomyopathy patients caused by heterozygous mutations in the HCN4 gene

Dilated cardiomyopathy (DCM) is a progressive heart muscle disease that can culminate with heart failure and death. Mutations in several genes can cause DCM, including hyperpolarization-activated cyclic nucleotide-gated channel (HCN4), which has a critical function in the autonomic control of the he...

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Autores principales: Yildirim, Zehra, Kojic, Ana, Yan, Christopher D., Wu, Matthew A., Vagelos, Randall, Wu, Joseph C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9881502/
https://www.ncbi.nlm.nih.gov/pubmed/36332467
http://dx.doi.org/10.1016/j.scr.2022.102951
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author Yildirim, Zehra
Kojic, Ana
Yan, Christopher D.
Wu, Matthew A.
Vagelos, Randall
Wu, Joseph C.
author_facet Yildirim, Zehra
Kojic, Ana
Yan, Christopher D.
Wu, Matthew A.
Vagelos, Randall
Wu, Joseph C.
author_sort Yildirim, Zehra
collection PubMed
description Dilated cardiomyopathy (DCM) is a progressive heart muscle disease that can culminate with heart failure and death. Mutations in several genes can cause DCM, including hyperpolarization-activated cyclic nucleotide-gated channel (HCN4), which has a critical function in the autonomic control of the heart rate. Here, we generated two human induced pluripotent stem cell (iPSC) lines generated from two DCM patients carrying variants in the HCN4 gene (c.2587G > T and c.2846G > A). Both lines display normal karyotype, typical morphology of pluripotent stem cells, and differentiate into all three germ layers in vitro. These lines are valuable resources for studying the pathological mechanisms of DCM.
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spelling pubmed-98815022023-01-27 Generation of two induced pluripotent stem cell lines from dilated cardiomyopathy patients caused by heterozygous mutations in the HCN4 gene Yildirim, Zehra Kojic, Ana Yan, Christopher D. Wu, Matthew A. Vagelos, Randall Wu, Joseph C. Stem Cell Res Article Dilated cardiomyopathy (DCM) is a progressive heart muscle disease that can culminate with heart failure and death. Mutations in several genes can cause DCM, including hyperpolarization-activated cyclic nucleotide-gated channel (HCN4), which has a critical function in the autonomic control of the heart rate. Here, we generated two human induced pluripotent stem cell (iPSC) lines generated from two DCM patients carrying variants in the HCN4 gene (c.2587G > T and c.2846G > A). Both lines display normal karyotype, typical morphology of pluripotent stem cells, and differentiate into all three germ layers in vitro. These lines are valuable resources for studying the pathological mechanisms of DCM. 2022-12 2022-10-19 /pmc/articles/PMC9881502/ /pubmed/36332467 http://dx.doi.org/10.1016/j.scr.2022.102951 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ).
spellingShingle Article
Yildirim, Zehra
Kojic, Ana
Yan, Christopher D.
Wu, Matthew A.
Vagelos, Randall
Wu, Joseph C.
Generation of two induced pluripotent stem cell lines from dilated cardiomyopathy patients caused by heterozygous mutations in the HCN4 gene
title Generation of two induced pluripotent stem cell lines from dilated cardiomyopathy patients caused by heterozygous mutations in the HCN4 gene
title_full Generation of two induced pluripotent stem cell lines from dilated cardiomyopathy patients caused by heterozygous mutations in the HCN4 gene
title_fullStr Generation of two induced pluripotent stem cell lines from dilated cardiomyopathy patients caused by heterozygous mutations in the HCN4 gene
title_full_unstemmed Generation of two induced pluripotent stem cell lines from dilated cardiomyopathy patients caused by heterozygous mutations in the HCN4 gene
title_short Generation of two induced pluripotent stem cell lines from dilated cardiomyopathy patients caused by heterozygous mutations in the HCN4 gene
title_sort generation of two induced pluripotent stem cell lines from dilated cardiomyopathy patients caused by heterozygous mutations in the hcn4 gene
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9881502/
https://www.ncbi.nlm.nih.gov/pubmed/36332467
http://dx.doi.org/10.1016/j.scr.2022.102951
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