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Radiopaque Implantable Biomaterials for Nerve Repair

Repairing peripheral nerve injuries remains a clinical challenge. To enhance nerve regeneration and functional recovery, the use of auxiliary implantable biomaterial conduits has become widespread. After implantation, there is currently no way to assess the location or function of polymeric biomedic...

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Autores principales: Pawelec, Kendell M, Hix, Jeremy ML, Shapiro, Erik M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9881907/
https://www.ncbi.nlm.nih.gov/pubmed/36711915
http://dx.doi.org/10.1101/2023.01.05.522860
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author Pawelec, Kendell M
Hix, Jeremy ML
Shapiro, Erik M
author_facet Pawelec, Kendell M
Hix, Jeremy ML
Shapiro, Erik M
author_sort Pawelec, Kendell M
collection PubMed
description Repairing peripheral nerve injuries remains a clinical challenge. To enhance nerve regeneration and functional recovery, the use of auxiliary implantable biomaterial conduits has become widespread. After implantation, there is currently no way to assess the location or function of polymeric biomedical devices, as they cannot be easily differentiated from surrounding tissue using clinical imaging modalities. Adding nanoparticle contrast agents into polymer matrices can introduce radiopacity and enable imaging using computed tomography (CT), but radiopacity must be balanced with changes in material properties that impact device function and biological response. In this study radiopacity was introduced to porous films of polycaprolactone (PCL) and poly(lactide-co-glycolide) (PLGA) 50:50 and 85:15 with 0–40wt% biocompatible tantalum oxide (TaO(x)) nanoparticles. To achieve radiopacity, at least 5wt% TaO(x) was required, with ≥ 20wt% TaO(x) leading to reduced mechanical properties and increased nano-scale surface roughness of films. As polymers used for peripheral nerve injury devices, films facilitated nerve regeneration in an in vitro co-culture model of glia (Schwann cells) and dorsal root ganglion neurons (DRG), measured by expression markers for myelination. The ability of radiopaque films to support nerve regeneration was determined by the properties of the polymer matrix, with a range of 5–20wt% TaO(x) balancing both imaging functionality with biological response and proving that in situ monitoring of nerve repair devices is feasible.
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spelling pubmed-98819072023-01-28 Radiopaque Implantable Biomaterials for Nerve Repair Pawelec, Kendell M Hix, Jeremy ML Shapiro, Erik M bioRxiv Article Repairing peripheral nerve injuries remains a clinical challenge. To enhance nerve regeneration and functional recovery, the use of auxiliary implantable biomaterial conduits has become widespread. After implantation, there is currently no way to assess the location or function of polymeric biomedical devices, as they cannot be easily differentiated from surrounding tissue using clinical imaging modalities. Adding nanoparticle contrast agents into polymer matrices can introduce radiopacity and enable imaging using computed tomography (CT), but radiopacity must be balanced with changes in material properties that impact device function and biological response. In this study radiopacity was introduced to porous films of polycaprolactone (PCL) and poly(lactide-co-glycolide) (PLGA) 50:50 and 85:15 with 0–40wt% biocompatible tantalum oxide (TaO(x)) nanoparticles. To achieve radiopacity, at least 5wt% TaO(x) was required, with ≥ 20wt% TaO(x) leading to reduced mechanical properties and increased nano-scale surface roughness of films. As polymers used for peripheral nerve injury devices, films facilitated nerve regeneration in an in vitro co-culture model of glia (Schwann cells) and dorsal root ganglion neurons (DRG), measured by expression markers for myelination. The ability of radiopaque films to support nerve regeneration was determined by the properties of the polymer matrix, with a range of 5–20wt% TaO(x) balancing both imaging functionality with biological response and proving that in situ monitoring of nerve repair devices is feasible. Cold Spring Harbor Laboratory 2023-01-06 /pmc/articles/PMC9881907/ /pubmed/36711915 http://dx.doi.org/10.1101/2023.01.05.522860 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator.
spellingShingle Article
Pawelec, Kendell M
Hix, Jeremy ML
Shapiro, Erik M
Radiopaque Implantable Biomaterials for Nerve Repair
title Radiopaque Implantable Biomaterials for Nerve Repair
title_full Radiopaque Implantable Biomaterials for Nerve Repair
title_fullStr Radiopaque Implantable Biomaterials for Nerve Repair
title_full_unstemmed Radiopaque Implantable Biomaterials for Nerve Repair
title_short Radiopaque Implantable Biomaterials for Nerve Repair
title_sort radiopaque implantable biomaterials for nerve repair
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9881907/
https://www.ncbi.nlm.nih.gov/pubmed/36711915
http://dx.doi.org/10.1101/2023.01.05.522860
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