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A novel, lightweight drive implant for chronic tetrode recordings in juvenile mice

In vivo electrophysiology provides unparalleled insight into sub-second-level circuit dynamics of the intact brain and represents a method of particular importance for studying mouse models of human neuro-psychiatric disorders. However, such methods often require large cranial implants which cannot...

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Autores principales: Pendry, Robert J, Quigley, Lilyana D, Volk, Lenora J, Pfeiffer, Brad E
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9881909/
https://www.ncbi.nlm.nih.gov/pubmed/36711560
http://dx.doi.org/10.1101/2023.01.04.522760
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author Pendry, Robert J
Quigley, Lilyana D
Volk, Lenora J
Pfeiffer, Brad E
author_facet Pendry, Robert J
Quigley, Lilyana D
Volk, Lenora J
Pfeiffer, Brad E
author_sort Pendry, Robert J
collection PubMed
description In vivo electrophysiology provides unparalleled insight into sub-second-level circuit dynamics of the intact brain and represents a method of particular importance for studying mouse models of human neuro-psychiatric disorders. However, such methods often require large cranial implants which cannot be used in mice at early developmental timepoints. As such, virtually no studies of in vivo physiology have been performed in freely behaving infant or juvenile mice, despite the fact that a better understanding of neurological development in this critical window is likely to provide unique insights into age-dependent developmental disorders such as autism or schizophrenia. Here, we describe a novel micro-drive design, surgical implantation procedure, and post-surgery recovery strategy that allows for chronic field and single-unit recordings from up to sixteen brain regions simultaneously in mice as they age from postnatal day 20 (p20) to postnatal day 60 (p60) and beyond, a time window roughly corresponding to human ages 2-years-old through adult. The number of recording electrodes and final recording sites can be easily modified and expanded, allowing flexible experimental control of in vivo monitoring of behavior- or disease-relevant brain regions across development.
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spelling pubmed-98819092023-01-28 A novel, lightweight drive implant for chronic tetrode recordings in juvenile mice Pendry, Robert J Quigley, Lilyana D Volk, Lenora J Pfeiffer, Brad E bioRxiv Article In vivo electrophysiology provides unparalleled insight into sub-second-level circuit dynamics of the intact brain and represents a method of particular importance for studying mouse models of human neuro-psychiatric disorders. However, such methods often require large cranial implants which cannot be used in mice at early developmental timepoints. As such, virtually no studies of in vivo physiology have been performed in freely behaving infant or juvenile mice, despite the fact that a better understanding of neurological development in this critical window is likely to provide unique insights into age-dependent developmental disorders such as autism or schizophrenia. Here, we describe a novel micro-drive design, surgical implantation procedure, and post-surgery recovery strategy that allows for chronic field and single-unit recordings from up to sixteen brain regions simultaneously in mice as they age from postnatal day 20 (p20) to postnatal day 60 (p60) and beyond, a time window roughly corresponding to human ages 2-years-old through adult. The number of recording electrodes and final recording sites can be easily modified and expanded, allowing flexible experimental control of in vivo monitoring of behavior- or disease-relevant brain regions across development. Cold Spring Harbor Laboratory 2023-01-05 /pmc/articles/PMC9881909/ /pubmed/36711560 http://dx.doi.org/10.1101/2023.01.04.522760 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator.
spellingShingle Article
Pendry, Robert J
Quigley, Lilyana D
Volk, Lenora J
Pfeiffer, Brad E
A novel, lightweight drive implant for chronic tetrode recordings in juvenile mice
title A novel, lightweight drive implant for chronic tetrode recordings in juvenile mice
title_full A novel, lightweight drive implant for chronic tetrode recordings in juvenile mice
title_fullStr A novel, lightweight drive implant for chronic tetrode recordings in juvenile mice
title_full_unstemmed A novel, lightweight drive implant for chronic tetrode recordings in juvenile mice
title_short A novel, lightweight drive implant for chronic tetrode recordings in juvenile mice
title_sort novel, lightweight drive implant for chronic tetrode recordings in juvenile mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9881909/
https://www.ncbi.nlm.nih.gov/pubmed/36711560
http://dx.doi.org/10.1101/2023.01.04.522760
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