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Increased mesoscale diffusivity in response to acute glucose starvation
Macromolecular crowding is an important parameter that impacts multiple biological processes. Passive microrheology using single particle tracking is a powerful means of studying macromolecular crowding. Here we monitored the diffusivity of self-assembling fluorescent nanoparticles (μNS) in response...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9882054/ https://www.ncbi.nlm.nih.gov/pubmed/36711511 http://dx.doi.org/10.1101/2023.01.10.523352 |
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author | Xie, Ying Gresham, David Holt, Liam |
author_facet | Xie, Ying Gresham, David Holt, Liam |
author_sort | Xie, Ying |
collection | PubMed |
description | Macromolecular crowding is an important parameter that impacts multiple biological processes. Passive microrheology using single particle tracking is a powerful means of studying macromolecular crowding. Here we monitored the diffusivity of self-assembling fluorescent nanoparticles (μNS) in response to acute glucose starvation. mRNP diffusivity was reduced upon glucose starvation as previously reported. In contrast, we observed increased diffusivity of μNS particles. Our results suggest that, upon glucose starvation, mRNP granule diffusivity may be reduced due to changes in physical interactions, while global crowding in the cytoplasm may be reduced. |
format | Online Article Text |
id | pubmed-9882054 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Cold Spring Harbor Laboratory |
record_format | MEDLINE/PubMed |
spelling | pubmed-98820542023-01-28 Increased mesoscale diffusivity in response to acute glucose starvation Xie, Ying Gresham, David Holt, Liam bioRxiv Article Macromolecular crowding is an important parameter that impacts multiple biological processes. Passive microrheology using single particle tracking is a powerful means of studying macromolecular crowding. Here we monitored the diffusivity of self-assembling fluorescent nanoparticles (μNS) in response to acute glucose starvation. mRNP diffusivity was reduced upon glucose starvation as previously reported. In contrast, we observed increased diffusivity of μNS particles. Our results suggest that, upon glucose starvation, mRNP granule diffusivity may be reduced due to changes in physical interactions, while global crowding in the cytoplasm may be reduced. Cold Spring Harbor Laboratory 2023-01-12 /pmc/articles/PMC9882054/ /pubmed/36711511 http://dx.doi.org/10.1101/2023.01.10.523352 Text en https://creativecommons.org/licenses/by-nd/4.0/This work is licensed under a Creative Commons Attribution-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, and only so long as attribution is given to the creator. The license allows for commercial use. |
spellingShingle | Article Xie, Ying Gresham, David Holt, Liam Increased mesoscale diffusivity in response to acute glucose starvation |
title | Increased mesoscale diffusivity in response to acute glucose starvation |
title_full | Increased mesoscale diffusivity in response to acute glucose starvation |
title_fullStr | Increased mesoscale diffusivity in response to acute glucose starvation |
title_full_unstemmed | Increased mesoscale diffusivity in response to acute glucose starvation |
title_short | Increased mesoscale diffusivity in response to acute glucose starvation |
title_sort | increased mesoscale diffusivity in response to acute glucose starvation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9882054/ https://www.ncbi.nlm.nih.gov/pubmed/36711511 http://dx.doi.org/10.1101/2023.01.10.523352 |
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