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The MOM1 complex recruits the RdDM machinery via MORC6 to establish de novo DNA methylation
MOM1 is an Arabidopsis factor previously shown to mediate transcriptional silencing independent of major DNA methylation changes. Here we found that MOM1 localizes with sites of RNA-directed DNA methylation (RdDM). Tethering MOM1 with artificial zinc finger to unmethylated FWA promoter led to establ...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9882083/ https://www.ncbi.nlm.nih.gov/pubmed/36711532 http://dx.doi.org/10.1101/2023.01.10.523455 |
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author | Li, Zheng Wang, Ming Zhong, Zhenhui Gallego-Bartolomé, Javier Feng, Suhua Jami-Alahmadi, Yasaman Wang, Xinyi Wohlschlegel, James Bischof, Sylvain Long, Jeffrey A. Jacobsen, Steven E. |
author_facet | Li, Zheng Wang, Ming Zhong, Zhenhui Gallego-Bartolomé, Javier Feng, Suhua Jami-Alahmadi, Yasaman Wang, Xinyi Wohlschlegel, James Bischof, Sylvain Long, Jeffrey A. Jacobsen, Steven E. |
author_sort | Li, Zheng |
collection | PubMed |
description | MOM1 is an Arabidopsis factor previously shown to mediate transcriptional silencing independent of major DNA methylation changes. Here we found that MOM1 localizes with sites of RNA-directed DNA methylation (RdDM). Tethering MOM1 with artificial zinc finger to unmethylated FWA promoter led to establishment of DNA methylation and FWA silencing. This process was blocked by mutations in components of the Pol V arm of the RdDM machinery, as well as by mutation of MORC6. We found that at some endogenous RdDM sites, MOM1 is required to maintain DNA methylation and a closed chromatin state. In addition, efficient silencing of newly introduced FWA transgenes was impaired by mutation of MOM1 or mutation of genes encoding the MOM1 interacting PIAL1/2 proteins. In addition to RdDM sites, we identified a group of MOM1 peaks at active chromatin near genes that colocalized with MORC6. These findings demonstrate a multifaceted role of MOM1 in genome regulation. |
format | Online Article Text |
id | pubmed-9882083 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Cold Spring Harbor Laboratory |
record_format | MEDLINE/PubMed |
spelling | pubmed-98820832023-01-28 The MOM1 complex recruits the RdDM machinery via MORC6 to establish de novo DNA methylation Li, Zheng Wang, Ming Zhong, Zhenhui Gallego-Bartolomé, Javier Feng, Suhua Jami-Alahmadi, Yasaman Wang, Xinyi Wohlschlegel, James Bischof, Sylvain Long, Jeffrey A. Jacobsen, Steven E. bioRxiv Article MOM1 is an Arabidopsis factor previously shown to mediate transcriptional silencing independent of major DNA methylation changes. Here we found that MOM1 localizes with sites of RNA-directed DNA methylation (RdDM). Tethering MOM1 with artificial zinc finger to unmethylated FWA promoter led to establishment of DNA methylation and FWA silencing. This process was blocked by mutations in components of the Pol V arm of the RdDM machinery, as well as by mutation of MORC6. We found that at some endogenous RdDM sites, MOM1 is required to maintain DNA methylation and a closed chromatin state. In addition, efficient silencing of newly introduced FWA transgenes was impaired by mutation of MOM1 or mutation of genes encoding the MOM1 interacting PIAL1/2 proteins. In addition to RdDM sites, we identified a group of MOM1 peaks at active chromatin near genes that colocalized with MORC6. These findings demonstrate a multifaceted role of MOM1 in genome regulation. Cold Spring Harbor Laboratory 2023-01-10 /pmc/articles/PMC9882083/ /pubmed/36711532 http://dx.doi.org/10.1101/2023.01.10.523455 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator. |
spellingShingle | Article Li, Zheng Wang, Ming Zhong, Zhenhui Gallego-Bartolomé, Javier Feng, Suhua Jami-Alahmadi, Yasaman Wang, Xinyi Wohlschlegel, James Bischof, Sylvain Long, Jeffrey A. Jacobsen, Steven E. The MOM1 complex recruits the RdDM machinery via MORC6 to establish de novo DNA methylation |
title | The MOM1 complex recruits the RdDM machinery via MORC6 to establish de novo DNA methylation |
title_full | The MOM1 complex recruits the RdDM machinery via MORC6 to establish de novo DNA methylation |
title_fullStr | The MOM1 complex recruits the RdDM machinery via MORC6 to establish de novo DNA methylation |
title_full_unstemmed | The MOM1 complex recruits the RdDM machinery via MORC6 to establish de novo DNA methylation |
title_short | The MOM1 complex recruits the RdDM machinery via MORC6 to establish de novo DNA methylation |
title_sort | mom1 complex recruits the rddm machinery via morc6 to establish de novo dna methylation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9882083/ https://www.ncbi.nlm.nih.gov/pubmed/36711532 http://dx.doi.org/10.1101/2023.01.10.523455 |
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