Interactive computational and experimental approaches improve the sensitivity of periplasmic binding protein-based nicotine biosensors for measurements in biofluids

We developed fluorescent protein sensors for nicotine with improved sensitivity. For iNicSnFR12 at pH 7.4, ΔF/F(0) increased with nicotine concentration; the proportionality constant (S-slope), 2.6 μM(−1), is 6.5-fold higher than the previously reported iNicSnFR3a. Fluorescence activation results pr...

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Detalles Bibliográficos
Autores principales: Haloi, Nandan, Huang, Shan, Nichols, Aaron L., Fine, Eve J., Friesenhahn, Nicholas J., Marotta, Christopher B., Dougherty, Dennis A., Lindahl, Erik, Howard, Rebecca J., Mayo, Stephen L., Lester, Henry A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9882114/
https://www.ncbi.nlm.nih.gov/pubmed/36712031
http://dx.doi.org/10.1101/2023.01.16.524298

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