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Non-photosynthetic bacteria produce photocurrent mediated by NADH

In recent years, the concern from the global climate change has driven an urgent need to develop clean energy technologies that do not involve combustion process that emit carbon into the atmosphere. A promising concept is microbial fuel cells that utilize bacteria as electron donors in a bio-electr...

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Autores principales: Shlosberg, Yaniv, Limwongyut, Jakkarin, Moreland, Alex S., Bazan, Guillermo C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9882120/
https://www.ncbi.nlm.nih.gov/pubmed/36711650
http://dx.doi.org/10.1101/2023.01.16.524302
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author Shlosberg, Yaniv
Limwongyut, Jakkarin
Moreland, Alex S.
Bazan, Guillermo C.
author_facet Shlosberg, Yaniv
Limwongyut, Jakkarin
Moreland, Alex S.
Bazan, Guillermo C.
author_sort Shlosberg, Yaniv
collection PubMed
description In recent years, the concern from the global climate change has driven an urgent need to develop clean energy technologies that do not involve combustion process that emit carbon into the atmosphere. A promising concept is microbial fuel cells that utilize bacteria as electron donors in a bio-electrochemical cell performing a direct electron transfer via conductive protein complexes or by secretion of redox active metabolites such as quinone or phenazine derivatives. In the case of photosynthetic bacteria (cyanobacteria) electrons can also be extracted from the photosynthetic pathway mediated mostly by NADH and NADPH. In this work, we show for the first time that the intact non-photosynthetic bacteria Escherichia coli can produce photocurrent that is enhanced upon addition of an exogenous electron mediator. Furthermore, we apply 2D-fluorescence measurement to show that NADH is released from the bacterial cells, which may apply as a native electron mediator in microbial fuel cells.
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spelling pubmed-98821202023-01-28 Non-photosynthetic bacteria produce photocurrent mediated by NADH Shlosberg, Yaniv Limwongyut, Jakkarin Moreland, Alex S. Bazan, Guillermo C. bioRxiv Article In recent years, the concern from the global climate change has driven an urgent need to develop clean energy technologies that do not involve combustion process that emit carbon into the atmosphere. A promising concept is microbial fuel cells that utilize bacteria as electron donors in a bio-electrochemical cell performing a direct electron transfer via conductive protein complexes or by secretion of redox active metabolites such as quinone or phenazine derivatives. In the case of photosynthetic bacteria (cyanobacteria) electrons can also be extracted from the photosynthetic pathway mediated mostly by NADH and NADPH. In this work, we show for the first time that the intact non-photosynthetic bacteria Escherichia coli can produce photocurrent that is enhanced upon addition of an exogenous electron mediator. Furthermore, we apply 2D-fluorescence measurement to show that NADH is released from the bacterial cells, which may apply as a native electron mediator in microbial fuel cells. Cold Spring Harbor Laboratory 2023-01-19 /pmc/articles/PMC9882120/ /pubmed/36711650 http://dx.doi.org/10.1101/2023.01.16.524302 Text en https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/) , which allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use.
spellingShingle Article
Shlosberg, Yaniv
Limwongyut, Jakkarin
Moreland, Alex S.
Bazan, Guillermo C.
Non-photosynthetic bacteria produce photocurrent mediated by NADH
title Non-photosynthetic bacteria produce photocurrent mediated by NADH
title_full Non-photosynthetic bacteria produce photocurrent mediated by NADH
title_fullStr Non-photosynthetic bacteria produce photocurrent mediated by NADH
title_full_unstemmed Non-photosynthetic bacteria produce photocurrent mediated by NADH
title_short Non-photosynthetic bacteria produce photocurrent mediated by NADH
title_sort non-photosynthetic bacteria produce photocurrent mediated by nadh
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9882120/
https://www.ncbi.nlm.nih.gov/pubmed/36711650
http://dx.doi.org/10.1101/2023.01.16.524302
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