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Specificity Guides Interpretation: On H3K4 Methylation at Enhancers and Broad Promoters

In 2018, we used internally calibrated chromatin immunoprecipitation (ICeChIP) to find that many of the most commonly used antibodies against H3K4 methylforms had significant off-target binding, which compromised the findings of at least eight literature paradigms that used these antibodies for ChIP...

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Detalles Bibliográficos
Autores principales: Shah, Rohan N., Ruthenburg, Alexander J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9882130/
https://www.ncbi.nlm.nih.gov/pubmed/36711866
http://dx.doi.org/10.1101/2023.01.16.524067