Cost-effective DNA methylation profiling by FML-seq

Mostrar otras versiones (1)

Current methods for profiling DNA methylation require costly reagents, sequencing, or labor time. We introduce FML-seq, a sequencing library protocol that greatly reduces all these costs. Relative to other techniques tested on the same human cell lines, FML-seq produces similar measurements of absol...

Descripción completa

Detalles Bibliográficos
Autores principales: Foley, Joseph W., Zhu, Shirley X., West, Robert B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9882132/
https://www.ncbi.nlm.nih.gov/pubmed/36711549
http://dx.doi.org/10.1101/2023.01.13.523849
_version_ 1784879242223288320
author Foley, Joseph W.
Zhu, Shirley X.
West, Robert B.
author_facet Foley, Joseph W.
Zhu, Shirley X.
West, Robert B.
author_sort Foley, Joseph W.
collection PubMed
description Current methods for profiling DNA methylation require costly reagents, sequencing, or labor time. We introduce FML-seq, a sequencing library protocol that greatly reduces all these costs. Relative to other techniques tested on the same human cell lines, FML-seq produces similar measurements of absolute and differential cytosine methylation at a fraction of the price. FML-seq enables inexpensive, high-throughput experimental designs for large-scale epigenetics research projects.
format Online
Article
Text
id pubmed-9882132
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Cold Spring Harbor Laboratory
record_format MEDLINE/PubMed
spelling pubmed-98821322023-01-28 Cost-effective DNA methylation profiling by FML-seq Foley, Joseph W. Zhu, Shirley X. West, Robert B. bioRxiv Article Current methods for profiling DNA methylation require costly reagents, sequencing, or labor time. We introduce FML-seq, a sequencing library protocol that greatly reduces all these costs. Relative to other techniques tested on the same human cell lines, FML-seq produces similar measurements of absolute and differential cytosine methylation at a fraction of the price. FML-seq enables inexpensive, high-throughput experimental designs for large-scale epigenetics research projects. Cold Spring Harbor Laboratory 2023-07-17 /pmc/articles/PMC9882132/ /pubmed/36711549 http://dx.doi.org/10.1101/2023.01.13.523849 Text en https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/) , which allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use.
spellingShingle Article
Foley, Joseph W.
Zhu, Shirley X.
West, Robert B.
Cost-effective DNA methylation profiling by FML-seq
title Cost-effective DNA methylation profiling by FML-seq
title_full Cost-effective DNA methylation profiling by FML-seq
title_fullStr Cost-effective DNA methylation profiling by FML-seq
title_full_unstemmed Cost-effective DNA methylation profiling by FML-seq
title_short Cost-effective DNA methylation profiling by FML-seq
title_sort cost-effective dna methylation profiling by fml-seq
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9882132/
https://www.ncbi.nlm.nih.gov/pubmed/36711549
http://dx.doi.org/10.1101/2023.01.13.523849
work_keys_str_mv AT foleyjosephw costeffectivednamethylationprofilingbyfmlseq
AT zhushirleyx costeffectivednamethylationprofilingbyfmlseq
AT westrobertb costeffectivednamethylationprofilingbyfmlseq

Ejemplares similares