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Cuticular profiling of insecticide resistant Aedes aegypti

Insecticides have made great strides in reducing the global burden of vector-borne disease. Nonetheless, serious public health concerns remain because insecticide-resistant vector populations continue to spread globally. To circumvent insecticide resistance, it is essential to understand all contrib...

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Autores principales: Jacobs, Ella, Chrissian, Christine, Rankin-Turner, Stephanie, Wear, Maggie, Camacho, Emma, Scott, Jeff G., Broderick, Nichole A., McMeniman, Conor J., Stark, Ruth E., Casadevall, Arturo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9882251/
https://www.ncbi.nlm.nih.gov/pubmed/36712033
http://dx.doi.org/10.1101/2023.01.13.523989
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author Jacobs, Ella
Chrissian, Christine
Rankin-Turner, Stephanie
Wear, Maggie
Camacho, Emma
Scott, Jeff G.
Broderick, Nichole A.
McMeniman, Conor J.
Stark, Ruth E.
Casadevall, Arturo
author_facet Jacobs, Ella
Chrissian, Christine
Rankin-Turner, Stephanie
Wear, Maggie
Camacho, Emma
Scott, Jeff G.
Broderick, Nichole A.
McMeniman, Conor J.
Stark, Ruth E.
Casadevall, Arturo
author_sort Jacobs, Ella
collection PubMed
description Insecticides have made great strides in reducing the global burden of vector-borne disease. Nonetheless, serious public health concerns remain because insecticide-resistant vector populations continue to spread globally. To circumvent insecticide resistance, it is essential to understand all contributing mechanisms. Contact-based insecticides are absorbed through the insect cuticle, which is comprised mainly of chitin polysaccharides, cuticular proteins, hydrocarbons, and phenolic biopolymers sclerotin and melanin. Cuticle interface alterations can slow or prevent insecticide penetration in a phenomenon referred to as cuticular resistance. Cuticular resistance characterization of the yellow fever mosquito, Aedes aegypti, is lacking. In the current study, we utilized solid-state Nuclear Magnetic Resonance (ssNMR) spectroscopy, gas chromatography/mass spectrometry (GC-MS), and transmission electron microscopy (TEM) to gain insights into the cuticle composition of congenic cytochrome P450 monooxygenase insecticide resistant and susceptible Ae. aegypti. No differences in cuticular hydrocarbon content or phenolic biopolymer deposition were found. In contrast, we observed cuticle thickness of insecticide resistant Ae. aegypti increased over time and exhibited higher polysaccharide abundance. Moreover, we found these local cuticular changes correlated with global metabolic differences in the whole mosquito, suggesting the existence of novel cuticular resistance mechanisms in this major disease vector.
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spelling pubmed-98822512023-01-28 Cuticular profiling of insecticide resistant Aedes aegypti Jacobs, Ella Chrissian, Christine Rankin-Turner, Stephanie Wear, Maggie Camacho, Emma Scott, Jeff G. Broderick, Nichole A. McMeniman, Conor J. Stark, Ruth E. Casadevall, Arturo bioRxiv Article Insecticides have made great strides in reducing the global burden of vector-borne disease. Nonetheless, serious public health concerns remain because insecticide-resistant vector populations continue to spread globally. To circumvent insecticide resistance, it is essential to understand all contributing mechanisms. Contact-based insecticides are absorbed through the insect cuticle, which is comprised mainly of chitin polysaccharides, cuticular proteins, hydrocarbons, and phenolic biopolymers sclerotin and melanin. Cuticle interface alterations can slow or prevent insecticide penetration in a phenomenon referred to as cuticular resistance. Cuticular resistance characterization of the yellow fever mosquito, Aedes aegypti, is lacking. In the current study, we utilized solid-state Nuclear Magnetic Resonance (ssNMR) spectroscopy, gas chromatography/mass spectrometry (GC-MS), and transmission electron microscopy (TEM) to gain insights into the cuticle composition of congenic cytochrome P450 monooxygenase insecticide resistant and susceptible Ae. aegypti. No differences in cuticular hydrocarbon content or phenolic biopolymer deposition were found. In contrast, we observed cuticle thickness of insecticide resistant Ae. aegypti increased over time and exhibited higher polysaccharide abundance. Moreover, we found these local cuticular changes correlated with global metabolic differences in the whole mosquito, suggesting the existence of novel cuticular resistance mechanisms in this major disease vector. Cold Spring Harbor Laboratory 2023-01-13 /pmc/articles/PMC9882251/ /pubmed/36712033 http://dx.doi.org/10.1101/2023.01.13.523989 Text en https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/) , which allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use.
spellingShingle Article
Jacobs, Ella
Chrissian, Christine
Rankin-Turner, Stephanie
Wear, Maggie
Camacho, Emma
Scott, Jeff G.
Broderick, Nichole A.
McMeniman, Conor J.
Stark, Ruth E.
Casadevall, Arturo
Cuticular profiling of insecticide resistant Aedes aegypti
title Cuticular profiling of insecticide resistant Aedes aegypti
title_full Cuticular profiling of insecticide resistant Aedes aegypti
title_fullStr Cuticular profiling of insecticide resistant Aedes aegypti
title_full_unstemmed Cuticular profiling of insecticide resistant Aedes aegypti
title_short Cuticular profiling of insecticide resistant Aedes aegypti
title_sort cuticular profiling of insecticide resistant aedes aegypti
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9882251/
https://www.ncbi.nlm.nih.gov/pubmed/36712033
http://dx.doi.org/10.1101/2023.01.13.523989
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