A dual sgRNA library design to probe genetic modifiers using genome-wide CRISPRi screens

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Mapping genetic interactions is essential for determining gene function and defining novel biological pathways. We report a simple to use CRISPR interference (CRISPRi) based platform, compatible with Fluorescence Activated Cell Sorting (FACS)-based reporter screens, to query epistatic relationships...

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Detalles Bibliográficos
Autores principales: Guna, Alina, Page, Katharine R., Replogle, Joseph M., Esantsi, Theodore K., Wang, Maxine L., Weissman, Jonathan S., Voorhees, Rebecca M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9882262/
https://www.ncbi.nlm.nih.gov/pubmed/36711738
http://dx.doi.org/10.1101/2023.01.22.525086
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