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Characterization of 3D organotypic epithelial tissues reveals tonsil-specific differences in tonic interferon signaling
Three-dimensional (3D) culturing techniques can recapitulate the stratified nature of multicellular epithelial tissues. Organotypic 3D epithelial tissue culture methods have several applications, including the study of tissue development and function, drug discovery and toxicity testing, host-pathog...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9882319/ https://www.ncbi.nlm.nih.gov/pubmed/36711548 http://dx.doi.org/10.1101/2023.01.19.524743 |
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author | Jackson, Robert Rajadhyaksha, Esha V Loeffler, Reid S Flores, Caitlyn E Van Doorslaer, Koenraad |
author_facet | Jackson, Robert Rajadhyaksha, Esha V Loeffler, Reid S Flores, Caitlyn E Van Doorslaer, Koenraad |
author_sort | Jackson, Robert |
collection | PubMed |
description | Three-dimensional (3D) culturing techniques can recapitulate the stratified nature of multicellular epithelial tissues. Organotypic 3D epithelial tissue culture methods have several applications, including the study of tissue development and function, drug discovery and toxicity testing, host-pathogen interactions, and the development of tissue-engineered constructs for use in regenerative medicine. We grew 3D organotypic epithelial tissues from foreskin, cervix, and tonsil-derived primary cells and characterized the transcriptome of these in vitro tissue equivalents. Using the same 3D culturing method, all three tissues yielded stratified squamous epithelium, validated histologically using basal and superficial epithelial cell markers. The goal of this study was to use RNA-seq to compare gene expression patterns in these three types of epithelial tissues to gain a better understanding of the molecular mechanisms underlying their function and identify potential therapeutic targets for various diseases. Functional profiling by over-representation and gene set enrichment analysis revealed tissue-specific differences: i.e., cutaneous homeostasis and lipid metabolism in foreskin, extracellular matrix remodeling in cervix, and baseline innate immune differences in tonsil. Specifically, tonsillar epithelia may play an active role in shaping the immune microenvironment of the tonsil balancing inflammation and immune responses in the face of constant exposure to microbial insults. Overall, these data serve as a resource, with gene sets made available for the research community to explore, and as a foundation for understanding the epithelial heterogeneity and how it may impact their in vitro use. An online resource is available to investigate these data (https://viz.datascience.arizona.edu/3DEpiEx/). |
format | Online Article Text |
id | pubmed-9882319 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Cold Spring Harbor Laboratory |
record_format | MEDLINE/PubMed |
spelling | pubmed-98823192023-01-28 Characterization of 3D organotypic epithelial tissues reveals tonsil-specific differences in tonic interferon signaling Jackson, Robert Rajadhyaksha, Esha V Loeffler, Reid S Flores, Caitlyn E Van Doorslaer, Koenraad bioRxiv Article Three-dimensional (3D) culturing techniques can recapitulate the stratified nature of multicellular epithelial tissues. Organotypic 3D epithelial tissue culture methods have several applications, including the study of tissue development and function, drug discovery and toxicity testing, host-pathogen interactions, and the development of tissue-engineered constructs for use in regenerative medicine. We grew 3D organotypic epithelial tissues from foreskin, cervix, and tonsil-derived primary cells and characterized the transcriptome of these in vitro tissue equivalents. Using the same 3D culturing method, all three tissues yielded stratified squamous epithelium, validated histologically using basal and superficial epithelial cell markers. The goal of this study was to use RNA-seq to compare gene expression patterns in these three types of epithelial tissues to gain a better understanding of the molecular mechanisms underlying their function and identify potential therapeutic targets for various diseases. Functional profiling by over-representation and gene set enrichment analysis revealed tissue-specific differences: i.e., cutaneous homeostasis and lipid metabolism in foreskin, extracellular matrix remodeling in cervix, and baseline innate immune differences in tonsil. Specifically, tonsillar epithelia may play an active role in shaping the immune microenvironment of the tonsil balancing inflammation and immune responses in the face of constant exposure to microbial insults. Overall, these data serve as a resource, with gene sets made available for the research community to explore, and as a foundation for understanding the epithelial heterogeneity and how it may impact their in vitro use. An online resource is available to investigate these data (https://viz.datascience.arizona.edu/3DEpiEx/). Cold Spring Harbor Laboratory 2023-01-20 /pmc/articles/PMC9882319/ /pubmed/36711548 http://dx.doi.org/10.1101/2023.01.19.524743 Text en https://creativecommons.org/licenses/by-nc/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (https://creativecommons.org/licenses/by-nc/4.0/) , which allows reusers to distribute, remix, adapt, and build upon the material in any medium or format for noncommercial purposes only, and only so long as attribution is given to the creator. |
spellingShingle | Article Jackson, Robert Rajadhyaksha, Esha V Loeffler, Reid S Flores, Caitlyn E Van Doorslaer, Koenraad Characterization of 3D organotypic epithelial tissues reveals tonsil-specific differences in tonic interferon signaling |
title | Characterization of 3D organotypic epithelial tissues reveals tonsil-specific differences in tonic interferon signaling |
title_full | Characterization of 3D organotypic epithelial tissues reveals tonsil-specific differences in tonic interferon signaling |
title_fullStr | Characterization of 3D organotypic epithelial tissues reveals tonsil-specific differences in tonic interferon signaling |
title_full_unstemmed | Characterization of 3D organotypic epithelial tissues reveals tonsil-specific differences in tonic interferon signaling |
title_short | Characterization of 3D organotypic epithelial tissues reveals tonsil-specific differences in tonic interferon signaling |
title_sort | characterization of 3d organotypic epithelial tissues reveals tonsil-specific differences in tonic interferon signaling |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9882319/ https://www.ncbi.nlm.nih.gov/pubmed/36711548 http://dx.doi.org/10.1101/2023.01.19.524743 |
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