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Transposon mutagenesis libraries reveal novel molecular requirements during CRISPR RNA-guided DNA integration

CRISPR-associated transposons (CASTs) direct DNA integration downstream of target sites using the RNA-guided DNA binding activity of nuclease-deficient CRISPR-Cas systems. Transposition relies on several key protein-protein and protein-DNA interactions, but little is known about the explicit sequenc...

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Detalles Bibliográficos
Autores principales:	Walker, Matt W.G., Klompe, Sanne E., Zhang, Dennis J., Sternberg, Samuel H.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Cold Spring Harbor Laboratory 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9882353/ https://www.ncbi.nlm.nih.gov/pubmed/36711804 http://dx.doi.org/10.1101/2023.01.19.524723

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