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Focused ultrasound-mediated brain genome editing
Gene editing in the mammalian brain has been challenging because of the restricted transport imposed by the blood-brain barrier (BBB). Current approaches rely on local injection to bypass the BBB. However, such administration is highly invasive and not amenable to treating certain delicate regions o...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American Journal Experts
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9882596/ https://www.ncbi.nlm.nih.gov/pubmed/36712096 http://dx.doi.org/10.21203/rs.3.rs-2365576/v1 |
| _version_ | 1784879324494561280 |
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| author | Leong, Kam Lao, Yeh-Hsing Ji, Robin Zhou, Joyce Snow, Kathy Kwon, Nancy Saville, Ethan He, Siyu Chauhan, Shradha Chi, Chun-Wei Datta, Malika Zhang, Hairong Quek, Chai Hoon Cai, Sarah Li, Mingqiang Gaitan, Yaned Bechtel, Lawrence Wu, Shih-Ying Lutz, Cathleen Tomer, Raju Murray, Stephen Chavez, Alejandro Konofagou, Elisa |
| author_facet | Leong, Kam Lao, Yeh-Hsing Ji, Robin Zhou, Joyce Snow, Kathy Kwon, Nancy Saville, Ethan He, Siyu Chauhan, Shradha Chi, Chun-Wei Datta, Malika Zhang, Hairong Quek, Chai Hoon Cai, Sarah Li, Mingqiang Gaitan, Yaned Bechtel, Lawrence Wu, Shih-Ying Lutz, Cathleen Tomer, Raju Murray, Stephen Chavez, Alejandro Konofagou, Elisa |
| author_sort | Leong, Kam |
| collection | PubMed |
| description | Gene editing in the mammalian brain has been challenging because of the restricted transport imposed by the blood-brain barrier (BBB). Current approaches rely on local injection to bypass the BBB. However, such administration is highly invasive and not amenable to treating certain delicate regions of the brain. We demonstrate a safe and effective gene editing technique by using focused ultrasound (FUS) to transiently open the BBB for the transport of intravenously delivered CRISPR/Cas9 machinery to the brain. |
| format | Online Article Text |
| id | pubmed-9882596 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2023 |
| publisher | American Journal Experts |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-98825962023-01-28 Focused ultrasound-mediated brain genome editing Leong, Kam Lao, Yeh-Hsing Ji, Robin Zhou, Joyce Snow, Kathy Kwon, Nancy Saville, Ethan He, Siyu Chauhan, Shradha Chi, Chun-Wei Datta, Malika Zhang, Hairong Quek, Chai Hoon Cai, Sarah Li, Mingqiang Gaitan, Yaned Bechtel, Lawrence Wu, Shih-Ying Lutz, Cathleen Tomer, Raju Murray, Stephen Chavez, Alejandro Konofagou, Elisa Res Sq Article Gene editing in the mammalian brain has been challenging because of the restricted transport imposed by the blood-brain barrier (BBB). Current approaches rely on local injection to bypass the BBB. However, such administration is highly invasive and not amenable to treating certain delicate regions of the brain. We demonstrate a safe and effective gene editing technique by using focused ultrasound (FUS) to transiently open the BBB for the transport of intravenously delivered CRISPR/Cas9 machinery to the brain. American Journal Experts 2023-01-20 /pmc/articles/PMC9882596/ /pubmed/36712096 http://dx.doi.org/10.21203/rs.3.rs-2365576/v1 Text en https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/) , which allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use. |
| spellingShingle | Article Leong, Kam Lao, Yeh-Hsing Ji, Robin Zhou, Joyce Snow, Kathy Kwon, Nancy Saville, Ethan He, Siyu Chauhan, Shradha Chi, Chun-Wei Datta, Malika Zhang, Hairong Quek, Chai Hoon Cai, Sarah Li, Mingqiang Gaitan, Yaned Bechtel, Lawrence Wu, Shih-Ying Lutz, Cathleen Tomer, Raju Murray, Stephen Chavez, Alejandro Konofagou, Elisa Focused ultrasound-mediated brain genome editing |
| title | Focused ultrasound-mediated brain genome editing |
| title_full | Focused ultrasound-mediated brain genome editing |
| title_fullStr | Focused ultrasound-mediated brain genome editing |
| title_full_unstemmed | Focused ultrasound-mediated brain genome editing |
| title_short | Focused ultrasound-mediated brain genome editing |
| title_sort | focused ultrasound-mediated brain genome editing |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9882596/ https://www.ncbi.nlm.nih.gov/pubmed/36712096 http://dx.doi.org/10.21203/rs.3.rs-2365576/v1 |
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