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Red blood cell rheology during a complete blood count: A proof of concept
Counting and sizing blood cells in hematological analyzers is achieved using the Coulter principle. The cells flow in a micro-aperture in which a strong electrical field is imposed, so that an electrical perturbation, called pulse, is measured each time a cell crosses the orifice. The pulses are exp...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9882912/ https://www.ncbi.nlm.nih.gov/pubmed/36706122 http://dx.doi.org/10.1371/journal.pone.0280952 |
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author | Taraconat, Pierre Gineys, Jean-Philippe Isebe, Damien Nicoud, Franck Mendez, Simon |
author_facet | Taraconat, Pierre Gineys, Jean-Philippe Isebe, Damien Nicoud, Franck Mendez, Simon |
author_sort | Taraconat, Pierre |
collection | PubMed |
description | Counting and sizing blood cells in hematological analyzers is achieved using the Coulter principle. The cells flow in a micro-aperture in which a strong electrical field is imposed, so that an electrical perturbation, called pulse, is measured each time a cell crosses the orifice. The pulses are expected to contain information on the shape and deformability of Red Blood Cells (RBCs), since recent studies state that RBCs rotate and deform in the micro-orifice. By implementing a dedicated numerical model, the present study sheds light on a variety of cells dynamics, which leads to different associated pulse signatures. Furthermore, simulations provide new insights on how RBCs shapes and mechanical properties affect the measured signals. Those numerical observations are confirmed by experimental assays. Finally, specific features are introduced for assessing the most relevant characteristics from the various pulse signatures and shown to highlight RBCs alterations induced by drugs. In summary, this study paves the way to a characterization of RBC rheology by routine hematological instruments. |
format | Online Article Text |
id | pubmed-9882912 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-98829122023-01-28 Red blood cell rheology during a complete blood count: A proof of concept Taraconat, Pierre Gineys, Jean-Philippe Isebe, Damien Nicoud, Franck Mendez, Simon PLoS One Research Article Counting and sizing blood cells in hematological analyzers is achieved using the Coulter principle. The cells flow in a micro-aperture in which a strong electrical field is imposed, so that an electrical perturbation, called pulse, is measured each time a cell crosses the orifice. The pulses are expected to contain information on the shape and deformability of Red Blood Cells (RBCs), since recent studies state that RBCs rotate and deform in the micro-orifice. By implementing a dedicated numerical model, the present study sheds light on a variety of cells dynamics, which leads to different associated pulse signatures. Furthermore, simulations provide new insights on how RBCs shapes and mechanical properties affect the measured signals. Those numerical observations are confirmed by experimental assays. Finally, specific features are introduced for assessing the most relevant characteristics from the various pulse signatures and shown to highlight RBCs alterations induced by drugs. In summary, this study paves the way to a characterization of RBC rheology by routine hematological instruments. Public Library of Science 2023-01-27 /pmc/articles/PMC9882912/ /pubmed/36706122 http://dx.doi.org/10.1371/journal.pone.0280952 Text en © 2023 Taraconat et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Taraconat, Pierre Gineys, Jean-Philippe Isebe, Damien Nicoud, Franck Mendez, Simon Red blood cell rheology during a complete blood count: A proof of concept |
title | Red blood cell rheology during a complete blood count: A proof of concept |
title_full | Red blood cell rheology during a complete blood count: A proof of concept |
title_fullStr | Red blood cell rheology during a complete blood count: A proof of concept |
title_full_unstemmed | Red blood cell rheology during a complete blood count: A proof of concept |
title_short | Red blood cell rheology during a complete blood count: A proof of concept |
title_sort | red blood cell rheology during a complete blood count: a proof of concept |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9882912/ https://www.ncbi.nlm.nih.gov/pubmed/36706122 http://dx.doi.org/10.1371/journal.pone.0280952 |
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