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Red blood cell rheology during a complete blood count: A proof of concept

Counting and sizing blood cells in hematological analyzers is achieved using the Coulter principle. The cells flow in a micro-aperture in which a strong electrical field is imposed, so that an electrical perturbation, called pulse, is measured each time a cell crosses the orifice. The pulses are exp...

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Autores principales: Taraconat, Pierre, Gineys, Jean-Philippe, Isebe, Damien, Nicoud, Franck, Mendez, Simon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9882912/
https://www.ncbi.nlm.nih.gov/pubmed/36706122
http://dx.doi.org/10.1371/journal.pone.0280952
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author Taraconat, Pierre
Gineys, Jean-Philippe
Isebe, Damien
Nicoud, Franck
Mendez, Simon
author_facet Taraconat, Pierre
Gineys, Jean-Philippe
Isebe, Damien
Nicoud, Franck
Mendez, Simon
author_sort Taraconat, Pierre
collection PubMed
description Counting and sizing blood cells in hematological analyzers is achieved using the Coulter principle. The cells flow in a micro-aperture in which a strong electrical field is imposed, so that an electrical perturbation, called pulse, is measured each time a cell crosses the orifice. The pulses are expected to contain information on the shape and deformability of Red Blood Cells (RBCs), since recent studies state that RBCs rotate and deform in the micro-orifice. By implementing a dedicated numerical model, the present study sheds light on a variety of cells dynamics, which leads to different associated pulse signatures. Furthermore, simulations provide new insights on how RBCs shapes and mechanical properties affect the measured signals. Those numerical observations are confirmed by experimental assays. Finally, specific features are introduced for assessing the most relevant characteristics from the various pulse signatures and shown to highlight RBCs alterations induced by drugs. In summary, this study paves the way to a characterization of RBC rheology by routine hematological instruments.
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spelling pubmed-98829122023-01-28 Red blood cell rheology during a complete blood count: A proof of concept Taraconat, Pierre Gineys, Jean-Philippe Isebe, Damien Nicoud, Franck Mendez, Simon PLoS One Research Article Counting and sizing blood cells in hematological analyzers is achieved using the Coulter principle. The cells flow in a micro-aperture in which a strong electrical field is imposed, so that an electrical perturbation, called pulse, is measured each time a cell crosses the orifice. The pulses are expected to contain information on the shape and deformability of Red Blood Cells (RBCs), since recent studies state that RBCs rotate and deform in the micro-orifice. By implementing a dedicated numerical model, the present study sheds light on a variety of cells dynamics, which leads to different associated pulse signatures. Furthermore, simulations provide new insights on how RBCs shapes and mechanical properties affect the measured signals. Those numerical observations are confirmed by experimental assays. Finally, specific features are introduced for assessing the most relevant characteristics from the various pulse signatures and shown to highlight RBCs alterations induced by drugs. In summary, this study paves the way to a characterization of RBC rheology by routine hematological instruments. Public Library of Science 2023-01-27 /pmc/articles/PMC9882912/ /pubmed/36706122 http://dx.doi.org/10.1371/journal.pone.0280952 Text en © 2023 Taraconat et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Taraconat, Pierre
Gineys, Jean-Philippe
Isebe, Damien
Nicoud, Franck
Mendez, Simon
Red blood cell rheology during a complete blood count: A proof of concept
title Red blood cell rheology during a complete blood count: A proof of concept
title_full Red blood cell rheology during a complete blood count: A proof of concept
title_fullStr Red blood cell rheology during a complete blood count: A proof of concept
title_full_unstemmed Red blood cell rheology during a complete blood count: A proof of concept
title_short Red blood cell rheology during a complete blood count: A proof of concept
title_sort red blood cell rheology during a complete blood count: a proof of concept
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9882912/
https://www.ncbi.nlm.nih.gov/pubmed/36706122
http://dx.doi.org/10.1371/journal.pone.0280952
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