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Metabolic fluorine labeling and hotspot imaging of dynamic gut microbiota in mice

Real-time localization and microbial activity information of indigenous gut microbiota over an extended period of time remains a challenge with existing visualizing methods. Here, we report a metabolic fluorine labeling (MEFLA)–based strategy for monitoring the dynamic gut microbiota via (19)F magne...

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Autores principales: Chen, Dongxia, Guo, Junnan, Li, Ao, Sun, Chengjie, Lin, Huibin, Lin, Hongyu, Yang, Chaoyong, Wang, Wei, Gao, Jinhao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9882976/
https://www.ncbi.nlm.nih.gov/pubmed/36706178
http://dx.doi.org/10.1126/sciadv.abg6808
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author Chen, Dongxia
Guo, Junnan
Li, Ao
Sun, Chengjie
Lin, Huibin
Lin, Hongyu
Yang, Chaoyong
Wang, Wei
Gao, Jinhao
author_facet Chen, Dongxia
Guo, Junnan
Li, Ao
Sun, Chengjie
Lin, Huibin
Lin, Hongyu
Yang, Chaoyong
Wang, Wei
Gao, Jinhao
author_sort Chen, Dongxia
collection PubMed
description Real-time localization and microbial activity information of indigenous gut microbiota over an extended period of time remains a challenge with existing visualizing methods. Here, we report a metabolic fluorine labeling (MEFLA)–based strategy for monitoring the dynamic gut microbiota via (19)F magnetic resonance imaging ((19)F MRI). In situ labeling of different microbiota subgroups is achieved by using a panel of peptidoglycan-targeting MEFLA probes containing (19)F atoms of different chemical shifts, and subsequent real-time in vivo imaging is accomplished by multiplexed hotspot (19)F MRI with high sensitivity and unlimited penetration. Using this method, we realize extended visualization (>24 hours) of native gut microbes located at different intestinal sections and semiquantitative analysis of their metabolic dynamics modulated by various conditions, such as the host death and different β-lactam antibiotics. Our strategy holds great potential for noninvasive and real-time assessing of the metabolic activities and locations of the highly dynamic gut microbiota.
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spelling pubmed-98829762023-02-08 Metabolic fluorine labeling and hotspot imaging of dynamic gut microbiota in mice Chen, Dongxia Guo, Junnan Li, Ao Sun, Chengjie Lin, Huibin Lin, Hongyu Yang, Chaoyong Wang, Wei Gao, Jinhao Sci Adv Physical and Materials Sciences Real-time localization and microbial activity information of indigenous gut microbiota over an extended period of time remains a challenge with existing visualizing methods. Here, we report a metabolic fluorine labeling (MEFLA)–based strategy for monitoring the dynamic gut microbiota via (19)F magnetic resonance imaging ((19)F MRI). In situ labeling of different microbiota subgroups is achieved by using a panel of peptidoglycan-targeting MEFLA probes containing (19)F atoms of different chemical shifts, and subsequent real-time in vivo imaging is accomplished by multiplexed hotspot (19)F MRI with high sensitivity and unlimited penetration. Using this method, we realize extended visualization (>24 hours) of native gut microbes located at different intestinal sections and semiquantitative analysis of their metabolic dynamics modulated by various conditions, such as the host death and different β-lactam antibiotics. Our strategy holds great potential for noninvasive and real-time assessing of the metabolic activities and locations of the highly dynamic gut microbiota. American Association for the Advancement of Science 2023-01-27 /pmc/articles/PMC9882976/ /pubmed/36706178 http://dx.doi.org/10.1126/sciadv.abg6808 Text en Copyright © 2023 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (https://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
spellingShingle Physical and Materials Sciences
Chen, Dongxia
Guo, Junnan
Li, Ao
Sun, Chengjie
Lin, Huibin
Lin, Hongyu
Yang, Chaoyong
Wang, Wei
Gao, Jinhao
Metabolic fluorine labeling and hotspot imaging of dynamic gut microbiota in mice
title Metabolic fluorine labeling and hotspot imaging of dynamic gut microbiota in mice
title_full Metabolic fluorine labeling and hotspot imaging of dynamic gut microbiota in mice
title_fullStr Metabolic fluorine labeling and hotspot imaging of dynamic gut microbiota in mice
title_full_unstemmed Metabolic fluorine labeling and hotspot imaging of dynamic gut microbiota in mice
title_short Metabolic fluorine labeling and hotspot imaging of dynamic gut microbiota in mice
title_sort metabolic fluorine labeling and hotspot imaging of dynamic gut microbiota in mice
topic Physical and Materials Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9882976/
https://www.ncbi.nlm.nih.gov/pubmed/36706178
http://dx.doi.org/10.1126/sciadv.abg6808
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