Cargando…

Fast and Sensitive Detection of SARS-CoV-2 Nucleic Acid Using a Rapid Detection System Free of RNA Extraction

OBJECTIVES: To establish and evaluate the analytical and clinical performance of the Flash20 SARS-CoV-2 nucleic acid rapid detection system free of RNA extraction. METHODS: The limit of detection (LoD) was determined using a negative nasopharyngeal swab matrix spiked with different concentrations of...

Descripción completa

Detalles Bibliográficos
Autores principales:	Ma, Liang, Fan, Yanyan, Kong, Xiaomu, Jiang, Yongwei, Huang, Hong, Zhao, Meimei, Liu, Yi, Gao, Peng, Cui, Yong, Cao, Yongtong
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Hindawi 2023
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9883100/ https://www.ncbi.nlm.nih.gov/pubmed/36714173 http://dx.doi.org/10.1155/2023/8053524

Ejemplares similares

Discrimination of SARS-CoV-2 omicron variant and its lineages by rapid detection of immune-escape mutations in spike protein RBD using asymmetric PCR-based melting curve analysis
por: Kong, Xiaomu, et al.
Publicado: (2023)

Rapid detection of the irinotecan‐related UGT1A1*28 polymorphism by asymmetric PCR melting curve analysis using one fluorescent probe
por: Kong, Xiaomu, et al.
Publicado: (2022)

Association of AGTR1 A1166C and CYP2C9∗3 Gene Polymorphisms with the Antihypertensive Effect of Valsartan
por: Liu, Yi, et al.
Publicado: (2022)

Role of HLA class I and II alleles in susceptibility to ankylosing spondylitis in Chinese Han
por: Jiang, Yongwei, et al.
Publicado: (2023)

A Novel Stool Methylation Test for the Non-Invasive Screening of Gastric and Colorectal Cancer
por: Ma, Liang, et al.
Publicado: (2022)

Chinese Expert Consensus on the Nucleic Acid Detection of SARS-CoV-2
por: Liu, Zijie, et al.
Publicado: (2020)

Comparison of Rapid Nucleic Acid Extraction Methods for SARS-CoV-2 Detection by RT-qPCR
por: Silva, Lívia Mara, et al.
Publicado: (2022)

Recent advances in PCR-free nucleic acid detection for SARS-COV-2
por: Ma, Xiaowei, et al.
Publicado: (2022)

A Fast, Visual, and Instrument-free Platform Involving Rapid DNA Extraction, Chemical Heating, and Recombinase Aided Amplification for On-Site Nucleic Acid Detection
por: Wang, Xiao Fu, et al.
Publicado: (2021)

Rapid, multiplexed, and nucleic acid amplification-free detection of SARS-CoV-2 RNA using an electrochemical biosensor
por: Kashefi-Kheyrabadi, Leila, et al.
Publicado: (2022)

Nanozyme-strip for rapid and ultrasensitive nucleic acid detection of SARS-CoV-2
por: Meng, Xiangqin, et al.
Publicado: (2022)

Fast, cheap and sensitive: Homogenizer-based RNA extraction free method for SARS-CoV-2 detection by RT-qPCR
por: Ramírez-Córdova, Cristina, et al.
Publicado: (2023)

Multiple strategies to improve sensitivity, speed and robustness of isothermal nucleic acid amplification for rapid pathogen detection
por: Tong, Yanhong, et al.
Publicado: (2011)

Evaluation of six different rapid methods for nucleic acid detection of SARS‐COV‐2 virus
por: Mahmoud, Sally A., et al.
Publicado: (2021)

Automated amplification-free digital RNA detection platform for rapid and sensitive SARS-CoV-2 diagnosis
por: Shinoda, Hajime, et al.
Publicado: (2022)

Recent Advances of Representative Optical Biosensors for Rapid and Sensitive Diagnostics of SARS-CoV-2
por: Xu, Meimei, et al.
Publicado: (2022)

Advances in Simple, Rapid, and Contamination-Free Instantaneous Nucleic Acid Devices for Pathogen Detection
por: Wang, Yue, et al.
Publicado: (2023)

Rapid and Sensitive Isothermal Detection of Nucleic-acid Sequence by Multiple Cross Displacement Amplification
por: Wang, Yi, et al.
Publicado: (2015)

Rapid and Sensitive Detection of Repetitive Nucleic Acid Sequences Using Magnetically Modulated Biosensors
por: Margulis, Michael, et al.
Publicado: (2019)

Nanomechanical assay for ultrasensitive and rapid detection of SARS-CoV-2 based on peptide nucleic acid
por: Wang, Yu, et al.
Publicado: (2022)

Rapid Detection of Attomolar SARS-CoV-2 Nucleic Acids in All-Dielectric Metasurface Biosensors
por: Iwanaga, Masanobu
Publicado: (2022)

A NOVEL ULTRAFAST REAL-TIME PCR INSTRUMENT FOR RAPID DETECTION OF SARS-COV-2 NUCLEIC ACIDS IN 30 MINUTES
por: Tan, J., et al.
Publicado: (2023)

Synergistic Effect of the MTHFR C677T and EPHX2 G860A Polymorphism on the Increased Risk of Ischemic Stroke in Chinese Type 2 Diabetic Patients
por: Ma, Liang, et al.
Publicado: (2017)

Nucleic Acids for Ultra-Sensitive Protein Detection
por: Janssen, Kris P. F., et al.
Publicado: (2013)

Simple and Low‐Cost Sampling of Cell‐Free Nucleic Acids from Blood Plasma for Rapid and Sensitive Detection of Circulating Tumor DNA
por: Jin, Choong Eun, et al.
Publicado: (2018)

A fast, sensitive and cost-effective method for nucleic acid detection using non-radioactive probes
por: Viterbo, David, et al.
Publicado: (2018)

A Novel Lateral Flow Assay for Rapid and Sensitive Nucleic Acid Detection of Avibacterium paragallinarum
por: Huo, Caiyun, et al.
Publicado: (2021)

An integrated rapid nucleic acid detection assay based on recombinant polymerase amplification for SARS-CoV-2
por: Tang, Ying, et al.
Publicado: (2022)

A Label-Free Impedimetric Genosensor for the Nucleic Acid Amplification-Free Detection of Extracted RNA of Dengue Virus
por: Wu, Ching-Chou, et al.
Publicado: (2020)

Sensitive extraction-free SARS-CoV-2 RNA virus detection using a chelating resin
por: Guan, Bin, et al.
Publicado: (2021)

An instrument-free, programmable approach for nucleic acid detection
Publicado: (2023)

A novel method for early detection of colorectal cancer based on detection of methylation of two fragments of syndecan-2 (SDC2) in stool DNA
por: Ma, Liang, et al.
Publicado: (2022)

Sensitive, Fast, and Specific Immunoassays for Methyltestosterone Detection
por: Kong, Na, et al.
Publicado: (2015)

Automatic label-free immunoassay with high sensitivity for rapid detection of SARS-CoV-2 nucleocapsid protein based on chemiluminescent magnetic beads
por: Lyu, Aihua, et al.
Publicado: (2021)

Association of EPHX2 R287Q Polymorphism with Diabetic Nephropathy in Chinese Type 2 Diabetic Patients
por: Ma, Liang, et al.
Publicado: (2018)

Analytical sensitivity and clinical sensitivity of the three rapid antigen detection kits for detection of SARS-CoV-2 virus
por: Mak, Gannon CK, et al.
Publicado: (2020)

Biosensing strategies for the detection of SARS-CoV-2 nucleic acids
por: Tamborelli, Alejandro, et al.
Publicado: (2023)

Strategies That Facilitate Extraction-Free SARS-CoV-2 Nucleic Acid Amplification Tests
por: Delgado-Diaz, David J., et al.
Publicado: (2022)

Ultra-sensitive and rapid detection of nucleic acids and microorganisms in body fluids using single-molecule tethering
por: Cheng, Wen-Chih, et al.
Publicado: (2020)

Rapid and sensitive detection of SARS-CoV-2 antibodies by biolayer interferometry
por: Dzimianski, John V., et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_npgliq5pidp6m06cm91dk53ks8