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A modular vaccine platform enabled by decoration of bacterial outer membrane vesicles with biotinylated antigens
Engineered outer membrane vesicles (OMVs) derived from Gram-negative bacteria are a promising technology for the creation of non-infectious, nanoparticle vaccines against diverse pathogens. However, antigen display on OMVs can be difficult to control and highly variable due to bottlenecks in protein...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9883832/ https://www.ncbi.nlm.nih.gov/pubmed/36709333 http://dx.doi.org/10.1038/s41467-023-36101-2 |
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author | Weyant, Kevin B. Oloyede, Ayomide Pal, Sukumar Liao, Julie Jesus, Mariela Rivera-De Jaroentomeechai, Thapakorn Moeller, Tyler D. Hoang-Phou, Steven Gilmore, Sean F. Singh, Riya Pan, Daniel C. Putnam, David Locher, Christopher de la Maza, Luis M. Coleman, Matthew A. DeLisa, Matthew P. |
author_facet | Weyant, Kevin B. Oloyede, Ayomide Pal, Sukumar Liao, Julie Jesus, Mariela Rivera-De Jaroentomeechai, Thapakorn Moeller, Tyler D. Hoang-Phou, Steven Gilmore, Sean F. Singh, Riya Pan, Daniel C. Putnam, David Locher, Christopher de la Maza, Luis M. Coleman, Matthew A. DeLisa, Matthew P. |
author_sort | Weyant, Kevin B. |
collection | PubMed |
description | Engineered outer membrane vesicles (OMVs) derived from Gram-negative bacteria are a promising technology for the creation of non-infectious, nanoparticle vaccines against diverse pathogens. However, antigen display on OMVs can be difficult to control and highly variable due to bottlenecks in protein expression and localization to the outer membrane of the host cell, especially for bulky and/or complex antigens. Here, we describe a universal approach for avidin-based vaccine antigen crosslinking (AvidVax) whereby biotinylated antigens are linked to the exterior of OMVs whose surfaces are remodeled with multiple copies of a synthetic antigen-binding protein (SNAP) comprised of an outer membrane scaffold protein fused to a biotin-binding protein. We show that SNAP-OMVs can be readily decorated with a molecularly diverse array of biotinylated subunit antigens, including globular and membrane proteins, glycans and glycoconjugates, haptens, lipids, and short peptides. When the resulting OMV formulations are injected in mice, strong antigen-specific antibody responses are observed that depend on the physical coupling between the antigen and SNAP-OMV delivery vehicle. Overall, these results demonstrate AvidVax as a modular platform that enables rapid and simplified assembly of antigen-studded OMVs for application as vaccines against pathogenic threats. |
format | Online Article Text |
id | pubmed-9883832 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-98838322023-01-30 A modular vaccine platform enabled by decoration of bacterial outer membrane vesicles with biotinylated antigens Weyant, Kevin B. Oloyede, Ayomide Pal, Sukumar Liao, Julie Jesus, Mariela Rivera-De Jaroentomeechai, Thapakorn Moeller, Tyler D. Hoang-Phou, Steven Gilmore, Sean F. Singh, Riya Pan, Daniel C. Putnam, David Locher, Christopher de la Maza, Luis M. Coleman, Matthew A. DeLisa, Matthew P. Nat Commun Article Engineered outer membrane vesicles (OMVs) derived from Gram-negative bacteria are a promising technology for the creation of non-infectious, nanoparticle vaccines against diverse pathogens. However, antigen display on OMVs can be difficult to control and highly variable due to bottlenecks in protein expression and localization to the outer membrane of the host cell, especially for bulky and/or complex antigens. Here, we describe a universal approach for avidin-based vaccine antigen crosslinking (AvidVax) whereby biotinylated antigens are linked to the exterior of OMVs whose surfaces are remodeled with multiple copies of a synthetic antigen-binding protein (SNAP) comprised of an outer membrane scaffold protein fused to a biotin-binding protein. We show that SNAP-OMVs can be readily decorated with a molecularly diverse array of biotinylated subunit antigens, including globular and membrane proteins, glycans and glycoconjugates, haptens, lipids, and short peptides. When the resulting OMV formulations are injected in mice, strong antigen-specific antibody responses are observed that depend on the physical coupling between the antigen and SNAP-OMV delivery vehicle. Overall, these results demonstrate AvidVax as a modular platform that enables rapid and simplified assembly of antigen-studded OMVs for application as vaccines against pathogenic threats. Nature Publishing Group UK 2023-01-28 /pmc/articles/PMC9883832/ /pubmed/36709333 http://dx.doi.org/10.1038/s41467-023-36101-2 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Weyant, Kevin B. Oloyede, Ayomide Pal, Sukumar Liao, Julie Jesus, Mariela Rivera-De Jaroentomeechai, Thapakorn Moeller, Tyler D. Hoang-Phou, Steven Gilmore, Sean F. Singh, Riya Pan, Daniel C. Putnam, David Locher, Christopher de la Maza, Luis M. Coleman, Matthew A. DeLisa, Matthew P. A modular vaccine platform enabled by decoration of bacterial outer membrane vesicles with biotinylated antigens |
title | A modular vaccine platform enabled by decoration of bacterial outer membrane vesicles with biotinylated antigens |
title_full | A modular vaccine platform enabled by decoration of bacterial outer membrane vesicles with biotinylated antigens |
title_fullStr | A modular vaccine platform enabled by decoration of bacterial outer membrane vesicles with biotinylated antigens |
title_full_unstemmed | A modular vaccine platform enabled by decoration of bacterial outer membrane vesicles with biotinylated antigens |
title_short | A modular vaccine platform enabled by decoration of bacterial outer membrane vesicles with biotinylated antigens |
title_sort | modular vaccine platform enabled by decoration of bacterial outer membrane vesicles with biotinylated antigens |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9883832/ https://www.ncbi.nlm.nih.gov/pubmed/36709333 http://dx.doi.org/10.1038/s41467-023-36101-2 |
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