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Overview of single‐cell RNA sequencing analysis and its application to spermatogenesis research
BACKGROUND: Single‐cell transcriptomics allows parallel analysis of multiple cell types in tissues. Because testes comprise somatic cells and germ cells at various stages of spermatogenesis, single‐cell RNA sequencing is a powerful tool for investigating the complex process of spermatogenesis. Howev...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9884325/ https://www.ncbi.nlm.nih.gov/pubmed/36726594 http://dx.doi.org/10.1002/rmb2.12502 |
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author | Suzuki, Takahiro |
author_facet | Suzuki, Takahiro |
author_sort | Suzuki, Takahiro |
collection | PubMed |
description | BACKGROUND: Single‐cell transcriptomics allows parallel analysis of multiple cell types in tissues. Because testes comprise somatic cells and germ cells at various stages of spermatogenesis, single‐cell RNA sequencing is a powerful tool for investigating the complex process of spermatogenesis. However, single‐cell RNA sequencing analysis needs extensive knowledge of experimental technologies and bioinformatics, making it difficult for many, particularly experimental biologists and clinicians, to use it. METHODS: Aiming to make single‐cell RNA sequencing analysis familiar, this review article presents an overview of experimental and computational methods for single‐cell RNA sequencing analysis with a history of transcriptomics. In addition, combining the PubMed search and manual curation, this review also provides a summary of recent novel insights into human and mouse spermatogenesis obtained using single‐cell RNA sequencing analyses. MAIN FINDINGS: Single‐cell RNA sequencing identified mesenchymal cells and type II innate lymphoid cells as novel testicular cell types in the adult mouse testes, as well as detailed subtypes of germ cells. This review outlines recent discoveries into germ cell development and subtypes, somatic cell development, and cell–cell interactions. CONCLUSION: The findings on spermatogenesis obtained using single‐cell RNA sequencing may contribute to a deeper understanding of spermatogenesis and provide new directions for male fertility therapy. |
format | Online Article Text |
id | pubmed-9884325 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-98843252023-01-31 Overview of single‐cell RNA sequencing analysis and its application to spermatogenesis research Suzuki, Takahiro Reprod Med Biol Reviews BACKGROUND: Single‐cell transcriptomics allows parallel analysis of multiple cell types in tissues. Because testes comprise somatic cells and germ cells at various stages of spermatogenesis, single‐cell RNA sequencing is a powerful tool for investigating the complex process of spermatogenesis. However, single‐cell RNA sequencing analysis needs extensive knowledge of experimental technologies and bioinformatics, making it difficult for many, particularly experimental biologists and clinicians, to use it. METHODS: Aiming to make single‐cell RNA sequencing analysis familiar, this review article presents an overview of experimental and computational methods for single‐cell RNA sequencing analysis with a history of transcriptomics. In addition, combining the PubMed search and manual curation, this review also provides a summary of recent novel insights into human and mouse spermatogenesis obtained using single‐cell RNA sequencing analyses. MAIN FINDINGS: Single‐cell RNA sequencing identified mesenchymal cells and type II innate lymphoid cells as novel testicular cell types in the adult mouse testes, as well as detailed subtypes of germ cells. This review outlines recent discoveries into germ cell development and subtypes, somatic cell development, and cell–cell interactions. CONCLUSION: The findings on spermatogenesis obtained using single‐cell RNA sequencing may contribute to a deeper understanding of spermatogenesis and provide new directions for male fertility therapy. John Wiley and Sons Inc. 2023-01-28 /pmc/articles/PMC9884325/ /pubmed/36726594 http://dx.doi.org/10.1002/rmb2.12502 Text en © 2023 The Author. Reproductive Medicine and Biology published by John Wiley & Sons Australia, Ltd on behalf of Japan Society for Reproductive Medicine. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Reviews Suzuki, Takahiro Overview of single‐cell RNA sequencing analysis and its application to spermatogenesis research |
title | Overview of single‐cell RNA sequencing analysis and its application to spermatogenesis research |
title_full | Overview of single‐cell RNA sequencing analysis and its application to spermatogenesis research |
title_fullStr | Overview of single‐cell RNA sequencing analysis and its application to spermatogenesis research |
title_full_unstemmed | Overview of single‐cell RNA sequencing analysis and its application to spermatogenesis research |
title_short | Overview of single‐cell RNA sequencing analysis and its application to spermatogenesis research |
title_sort | overview of single‐cell rna sequencing analysis and its application to spermatogenesis research |
topic | Reviews |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9884325/ https://www.ncbi.nlm.nih.gov/pubmed/36726594 http://dx.doi.org/10.1002/rmb2.12502 |
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