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Estradiol-17β [E(2)] stimulates wound healing in a 3D in vitro tissue-engineered vaginal wound model

Childbirth contributes to common pelvic floor problems requiring reconstructive surgery in postmenopausal women. Our aim was to develop a tissue-engineered vaginal wound model to investigate wound healing and the contribution of estradiol to pelvic tissue repair. Partial thickness scalpel wounds wer...

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Autores principales: Shafaat, Sarah, Roman Regueros, Sabiniano, Chapple, Christopher, MacNeil, Sheila, Hearnden, Vanessa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9885031/
https://www.ncbi.nlm.nih.gov/pubmed/36726532
http://dx.doi.org/10.1177/20417314221149207
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author Shafaat, Sarah
Roman Regueros, Sabiniano
Chapple, Christopher
MacNeil, Sheila
Hearnden, Vanessa
author_facet Shafaat, Sarah
Roman Regueros, Sabiniano
Chapple, Christopher
MacNeil, Sheila
Hearnden, Vanessa
author_sort Shafaat, Sarah
collection PubMed
description Childbirth contributes to common pelvic floor problems requiring reconstructive surgery in postmenopausal women. Our aim was to develop a tissue-engineered vaginal wound model to investigate wound healing and the contribution of estradiol to pelvic tissue repair. Partial thickness scalpel wounds were made in tissue models based on decellularized sheep vaginal matrices cultured with primary sheep vaginal epithelial cells and fibroblasts. Models were cultured at an airliquid interface (ALI) for 3 weeks with and without estradiol-17β [E(2)]. Results showed that E(2) significantly increased wound healing and epithelial maturation. Also, E(2) led to collagen reorganization after only 14 days with collagen fibers more regularly aligned and compactly arranged Additionally, E(2) significantly downregulated α-SMA expression which is involved in fibrotic tissue formation. This model allows one to investigate multiple steps in vaginal wound healing and could be a useful tool in developing therapies for improved tissue healing after reconstructive pelvic floor surgery.
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spelling pubmed-98850312023-01-31 Estradiol-17β [E(2)] stimulates wound healing in a 3D in vitro tissue-engineered vaginal wound model Shafaat, Sarah Roman Regueros, Sabiniano Chapple, Christopher MacNeil, Sheila Hearnden, Vanessa J Tissue Eng Original Article Childbirth contributes to common pelvic floor problems requiring reconstructive surgery in postmenopausal women. Our aim was to develop a tissue-engineered vaginal wound model to investigate wound healing and the contribution of estradiol to pelvic tissue repair. Partial thickness scalpel wounds were made in tissue models based on decellularized sheep vaginal matrices cultured with primary sheep vaginal epithelial cells and fibroblasts. Models were cultured at an airliquid interface (ALI) for 3 weeks with and without estradiol-17β [E(2)]. Results showed that E(2) significantly increased wound healing and epithelial maturation. Also, E(2) led to collagen reorganization after only 14 days with collagen fibers more regularly aligned and compactly arranged Additionally, E(2) significantly downregulated α-SMA expression which is involved in fibrotic tissue formation. This model allows one to investigate multiple steps in vaginal wound healing and could be a useful tool in developing therapies for improved tissue healing after reconstructive pelvic floor surgery. SAGE Publications 2023-01-27 /pmc/articles/PMC9885031/ /pubmed/36726532 http://dx.doi.org/10.1177/20417314221149207 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Article
Shafaat, Sarah
Roman Regueros, Sabiniano
Chapple, Christopher
MacNeil, Sheila
Hearnden, Vanessa
Estradiol-17β [E(2)] stimulates wound healing in a 3D in vitro tissue-engineered vaginal wound model
title Estradiol-17β [E(2)] stimulates wound healing in a 3D in vitro tissue-engineered vaginal wound model
title_full Estradiol-17β [E(2)] stimulates wound healing in a 3D in vitro tissue-engineered vaginal wound model
title_fullStr Estradiol-17β [E(2)] stimulates wound healing in a 3D in vitro tissue-engineered vaginal wound model
title_full_unstemmed Estradiol-17β [E(2)] stimulates wound healing in a 3D in vitro tissue-engineered vaginal wound model
title_short Estradiol-17β [E(2)] stimulates wound healing in a 3D in vitro tissue-engineered vaginal wound model
title_sort estradiol-17β [e(2)] stimulates wound healing in a 3d in vitro tissue-engineered vaginal wound model
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9885031/
https://www.ncbi.nlm.nih.gov/pubmed/36726532
http://dx.doi.org/10.1177/20417314221149207
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