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Estradiol-17β [E(2)] stimulates wound healing in a 3D in vitro tissue-engineered vaginal wound model
Childbirth contributes to common pelvic floor problems requiring reconstructive surgery in postmenopausal women. Our aim was to develop a tissue-engineered vaginal wound model to investigate wound healing and the contribution of estradiol to pelvic tissue repair. Partial thickness scalpel wounds wer...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9885031/ https://www.ncbi.nlm.nih.gov/pubmed/36726532 http://dx.doi.org/10.1177/20417314221149207 |
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author | Shafaat, Sarah Roman Regueros, Sabiniano Chapple, Christopher MacNeil, Sheila Hearnden, Vanessa |
author_facet | Shafaat, Sarah Roman Regueros, Sabiniano Chapple, Christopher MacNeil, Sheila Hearnden, Vanessa |
author_sort | Shafaat, Sarah |
collection | PubMed |
description | Childbirth contributes to common pelvic floor problems requiring reconstructive surgery in postmenopausal women. Our aim was to develop a tissue-engineered vaginal wound model to investigate wound healing and the contribution of estradiol to pelvic tissue repair. Partial thickness scalpel wounds were made in tissue models based on decellularized sheep vaginal matrices cultured with primary sheep vaginal epithelial cells and fibroblasts. Models were cultured at an airliquid interface (ALI) for 3 weeks with and without estradiol-17β [E(2)]. Results showed that E(2) significantly increased wound healing and epithelial maturation. Also, E(2) led to collagen reorganization after only 14 days with collagen fibers more regularly aligned and compactly arranged Additionally, E(2) significantly downregulated α-SMA expression which is involved in fibrotic tissue formation. This model allows one to investigate multiple steps in vaginal wound healing and could be a useful tool in developing therapies for improved tissue healing after reconstructive pelvic floor surgery. |
format | Online Article Text |
id | pubmed-9885031 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-98850312023-01-31 Estradiol-17β [E(2)] stimulates wound healing in a 3D in vitro tissue-engineered vaginal wound model Shafaat, Sarah Roman Regueros, Sabiniano Chapple, Christopher MacNeil, Sheila Hearnden, Vanessa J Tissue Eng Original Article Childbirth contributes to common pelvic floor problems requiring reconstructive surgery in postmenopausal women. Our aim was to develop a tissue-engineered vaginal wound model to investigate wound healing and the contribution of estradiol to pelvic tissue repair. Partial thickness scalpel wounds were made in tissue models based on decellularized sheep vaginal matrices cultured with primary sheep vaginal epithelial cells and fibroblasts. Models were cultured at an airliquid interface (ALI) for 3 weeks with and without estradiol-17β [E(2)]. Results showed that E(2) significantly increased wound healing and epithelial maturation. Also, E(2) led to collagen reorganization after only 14 days with collagen fibers more regularly aligned and compactly arranged Additionally, E(2) significantly downregulated α-SMA expression which is involved in fibrotic tissue formation. This model allows one to investigate multiple steps in vaginal wound healing and could be a useful tool in developing therapies for improved tissue healing after reconstructive pelvic floor surgery. SAGE Publications 2023-01-27 /pmc/articles/PMC9885031/ /pubmed/36726532 http://dx.doi.org/10.1177/20417314221149207 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Original Article Shafaat, Sarah Roman Regueros, Sabiniano Chapple, Christopher MacNeil, Sheila Hearnden, Vanessa Estradiol-17β [E(2)] stimulates wound healing in a 3D in vitro tissue-engineered vaginal wound model |
title | Estradiol-17β [E(2)] stimulates wound healing in a 3D in
vitro tissue-engineered vaginal wound model |
title_full | Estradiol-17β [E(2)] stimulates wound healing in a 3D in
vitro tissue-engineered vaginal wound model |
title_fullStr | Estradiol-17β [E(2)] stimulates wound healing in a 3D in
vitro tissue-engineered vaginal wound model |
title_full_unstemmed | Estradiol-17β [E(2)] stimulates wound healing in a 3D in
vitro tissue-engineered vaginal wound model |
title_short | Estradiol-17β [E(2)] stimulates wound healing in a 3D in
vitro tissue-engineered vaginal wound model |
title_sort | estradiol-17β [e(2)] stimulates wound healing in a 3d in
vitro tissue-engineered vaginal wound model |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9885031/ https://www.ncbi.nlm.nih.gov/pubmed/36726532 http://dx.doi.org/10.1177/20417314221149207 |
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