Cargando…

Engineering Bacillus subtilis for production of 3-hydroxypropanoic acid

3-Hydroxypropionic acid (3-HP) is a valuable platform chemical that is used as a precursor for several higher value-added chemical products. There is an increased interest in development of cell factories as a means for the synthesis of 3-HP and various other platform chemicals. For more than a deca...

Descripción completa

Detalles Bibliográficos
Autores principales: Garg, Abhroop, Jers, Carsten, Hwang, Hee Jin, Kalantari, Aida, Ventina, Ildze, Mijakovic, Ivan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9885095/
https://www.ncbi.nlm.nih.gov/pubmed/36726744
http://dx.doi.org/10.3389/fbioe.2023.1101232
_version_ 1784879856516857856
author Garg, Abhroop
Jers, Carsten
Hwang, Hee Jin
Kalantari, Aida
Ventina, Ildze
Mijakovic, Ivan
author_facet Garg, Abhroop
Jers, Carsten
Hwang, Hee Jin
Kalantari, Aida
Ventina, Ildze
Mijakovic, Ivan
author_sort Garg, Abhroop
collection PubMed
description 3-Hydroxypropionic acid (3-HP) is a valuable platform chemical that is used as a precursor for several higher value-added chemical products. There is an increased interest in development of cell factories as a means for the synthesis of 3-HP and various other platform chemicals. For more than a decade, concentrated effort has been invested by the scientific community towards developing bio-based approaches for the production of 3-HP using primarily Escherichia coli and Klebsiella pneumoniae as production hosts. These hosts however might not be optimal for applications in e.g., food industry due primarily to endotoxin production and the pathogenic origin of particularly the K. pneumoniae. We have previously demonstrated that the generally recognized as safe organism Bacillus subtilis can be engineered to produce 3-HP using glycerol, an abundant by-product of the biodiesel industry, as substrate. For commercial exploitation, there is a need to substantially increase the titer. In the present study, we optimized the bioprocess conditions and further engineered the B. subtilis 3-HP production strain. Thereby, using glycerol as substrate, we were able to improve 3-HP production in a 1-L bioreactor to a final titer of 22.9 g/L 3-HP.
format Online
Article
Text
id pubmed-9885095
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-98850952023-01-31 Engineering Bacillus subtilis for production of 3-hydroxypropanoic acid Garg, Abhroop Jers, Carsten Hwang, Hee Jin Kalantari, Aida Ventina, Ildze Mijakovic, Ivan Front Bioeng Biotechnol Bioengineering and Biotechnology 3-Hydroxypropionic acid (3-HP) is a valuable platform chemical that is used as a precursor for several higher value-added chemical products. There is an increased interest in development of cell factories as a means for the synthesis of 3-HP and various other platform chemicals. For more than a decade, concentrated effort has been invested by the scientific community towards developing bio-based approaches for the production of 3-HP using primarily Escherichia coli and Klebsiella pneumoniae as production hosts. These hosts however might not be optimal for applications in e.g., food industry due primarily to endotoxin production and the pathogenic origin of particularly the K. pneumoniae. We have previously demonstrated that the generally recognized as safe organism Bacillus subtilis can be engineered to produce 3-HP using glycerol, an abundant by-product of the biodiesel industry, as substrate. For commercial exploitation, there is a need to substantially increase the titer. In the present study, we optimized the bioprocess conditions and further engineered the B. subtilis 3-HP production strain. Thereby, using glycerol as substrate, we were able to improve 3-HP production in a 1-L bioreactor to a final titer of 22.9 g/L 3-HP. Frontiers Media S.A. 2023-01-16 /pmc/articles/PMC9885095/ /pubmed/36726744 http://dx.doi.org/10.3389/fbioe.2023.1101232 Text en Copyright © 2023 Garg, Jers, Hwang, Kalantari, Ventina and Mijakovic. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Garg, Abhroop
Jers, Carsten
Hwang, Hee Jin
Kalantari, Aida
Ventina, Ildze
Mijakovic, Ivan
Engineering Bacillus subtilis for production of 3-hydroxypropanoic acid
title Engineering Bacillus subtilis for production of 3-hydroxypropanoic acid
title_full Engineering Bacillus subtilis for production of 3-hydroxypropanoic acid
title_fullStr Engineering Bacillus subtilis for production of 3-hydroxypropanoic acid
title_full_unstemmed Engineering Bacillus subtilis for production of 3-hydroxypropanoic acid
title_short Engineering Bacillus subtilis for production of 3-hydroxypropanoic acid
title_sort engineering bacillus subtilis for production of 3-hydroxypropanoic acid
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9885095/
https://www.ncbi.nlm.nih.gov/pubmed/36726744
http://dx.doi.org/10.3389/fbioe.2023.1101232
work_keys_str_mv AT gargabhroop engineeringbacillussubtilisforproductionof3hydroxypropanoicacid
AT jerscarsten engineeringbacillussubtilisforproductionof3hydroxypropanoicacid
AT hwangheejin engineeringbacillussubtilisforproductionof3hydroxypropanoicacid
AT kalantariaida engineeringbacillussubtilisforproductionof3hydroxypropanoicacid
AT ventinaildze engineeringbacillussubtilisforproductionof3hydroxypropanoicacid
AT mijakovicivan engineeringbacillussubtilisforproductionof3hydroxypropanoicacid