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Engineering Bacillus subtilis for production of 3-hydroxypropanoic acid
3-Hydroxypropionic acid (3-HP) is a valuable platform chemical that is used as a precursor for several higher value-added chemical products. There is an increased interest in development of cell factories as a means for the synthesis of 3-HP and various other platform chemicals. For more than a deca...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9885095/ https://www.ncbi.nlm.nih.gov/pubmed/36726744 http://dx.doi.org/10.3389/fbioe.2023.1101232 |
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author | Garg, Abhroop Jers, Carsten Hwang, Hee Jin Kalantari, Aida Ventina, Ildze Mijakovic, Ivan |
author_facet | Garg, Abhroop Jers, Carsten Hwang, Hee Jin Kalantari, Aida Ventina, Ildze Mijakovic, Ivan |
author_sort | Garg, Abhroop |
collection | PubMed |
description | 3-Hydroxypropionic acid (3-HP) is a valuable platform chemical that is used as a precursor for several higher value-added chemical products. There is an increased interest in development of cell factories as a means for the synthesis of 3-HP and various other platform chemicals. For more than a decade, concentrated effort has been invested by the scientific community towards developing bio-based approaches for the production of 3-HP using primarily Escherichia coli and Klebsiella pneumoniae as production hosts. These hosts however might not be optimal for applications in e.g., food industry due primarily to endotoxin production and the pathogenic origin of particularly the K. pneumoniae. We have previously demonstrated that the generally recognized as safe organism Bacillus subtilis can be engineered to produce 3-HP using glycerol, an abundant by-product of the biodiesel industry, as substrate. For commercial exploitation, there is a need to substantially increase the titer. In the present study, we optimized the bioprocess conditions and further engineered the B. subtilis 3-HP production strain. Thereby, using glycerol as substrate, we were able to improve 3-HP production in a 1-L bioreactor to a final titer of 22.9 g/L 3-HP. |
format | Online Article Text |
id | pubmed-9885095 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-98850952023-01-31 Engineering Bacillus subtilis for production of 3-hydroxypropanoic acid Garg, Abhroop Jers, Carsten Hwang, Hee Jin Kalantari, Aida Ventina, Ildze Mijakovic, Ivan Front Bioeng Biotechnol Bioengineering and Biotechnology 3-Hydroxypropionic acid (3-HP) is a valuable platform chemical that is used as a precursor for several higher value-added chemical products. There is an increased interest in development of cell factories as a means for the synthesis of 3-HP and various other platform chemicals. For more than a decade, concentrated effort has been invested by the scientific community towards developing bio-based approaches for the production of 3-HP using primarily Escherichia coli and Klebsiella pneumoniae as production hosts. These hosts however might not be optimal for applications in e.g., food industry due primarily to endotoxin production and the pathogenic origin of particularly the K. pneumoniae. We have previously demonstrated that the generally recognized as safe organism Bacillus subtilis can be engineered to produce 3-HP using glycerol, an abundant by-product of the biodiesel industry, as substrate. For commercial exploitation, there is a need to substantially increase the titer. In the present study, we optimized the bioprocess conditions and further engineered the B. subtilis 3-HP production strain. Thereby, using glycerol as substrate, we were able to improve 3-HP production in a 1-L bioreactor to a final titer of 22.9 g/L 3-HP. Frontiers Media S.A. 2023-01-16 /pmc/articles/PMC9885095/ /pubmed/36726744 http://dx.doi.org/10.3389/fbioe.2023.1101232 Text en Copyright © 2023 Garg, Jers, Hwang, Kalantari, Ventina and Mijakovic. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Garg, Abhroop Jers, Carsten Hwang, Hee Jin Kalantari, Aida Ventina, Ildze Mijakovic, Ivan Engineering Bacillus subtilis for production of 3-hydroxypropanoic acid |
title | Engineering Bacillus subtilis for production of 3-hydroxypropanoic acid |
title_full | Engineering Bacillus subtilis for production of 3-hydroxypropanoic acid |
title_fullStr | Engineering Bacillus subtilis for production of 3-hydroxypropanoic acid |
title_full_unstemmed | Engineering Bacillus subtilis for production of 3-hydroxypropanoic acid |
title_short | Engineering Bacillus subtilis for production of 3-hydroxypropanoic acid |
title_sort | engineering bacillus subtilis for production of 3-hydroxypropanoic acid |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9885095/ https://www.ncbi.nlm.nih.gov/pubmed/36726744 http://dx.doi.org/10.3389/fbioe.2023.1101232 |
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