Cargando…

Increased expression of long-isoform thymic stromal lymphopoietin is associated with rheumatoid arthritis and fosters inflammatory responses

Thymic stromal lymphopoietin (TSLP) is a pleiotropic cytokine that is involved in the pathogenesis of inflammatory diseases and asthma, but the expression and biological implications of the existence of two isoforms, long TSLP (lTSLP) and short TSLP (sTSLP), in RA have yet to be elucidated. Here we...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Wanlin, Liao, Chenghui, Du, Jing, Hu, Jing, Wang, Lu, Song, Xun, He, Zhendan, Xiao, Xiaohua, Ye, Liang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9885117/
https://www.ncbi.nlm.nih.gov/pubmed/36726974
http://dx.doi.org/10.3389/fimmu.2022.1079415
_version_ 1784879862012444672
author Li, Wanlin
Liao, Chenghui
Du, Jing
Hu, Jing
Wang, Lu
Song, Xun
He, Zhendan
Xiao, Xiaohua
Ye, Liang
author_facet Li, Wanlin
Liao, Chenghui
Du, Jing
Hu, Jing
Wang, Lu
Song, Xun
He, Zhendan
Xiao, Xiaohua
Ye, Liang
author_sort Li, Wanlin
collection PubMed
description Thymic stromal lymphopoietin (TSLP) is a pleiotropic cytokine that is involved in the pathogenesis of inflammatory diseases and asthma, but the expression and biological implications of the existence of two isoforms, long TSLP (lTSLP) and short TSLP (sTSLP), in RA have yet to be elucidated. Here we report that lTSLP is the predominant TSLP isoform in RA and active RA, whereas sTSLP is the major TSLP isoform in inactive RA and healthy controls. lTSLP expression is associated with disease activity, including 28-joint Disease Activity Score (DAS28) and erythrocyte sedimentation rate (ESR), as well as proinflammatory cytokine expression, irrespective of other laboratory parameters. Importantly, lTSLP alone or combined with LPS promotes the expression of proinflammatory cytokines IL-1β, IL-6, and IL-8 in PBMCs of RA, but restrains anti-inflammatory cytokine IL-10 expression in PBMCs of RA. Furthermore, we found that STAT5 signaling is involved in lTSLP-induced inflammatory accumulation in PBMCs of RA. Therefore, these results highlight the clinical significance of lTSLP in RA pathology and inflammatory response in acute-phase disease, which may provide a therapeutic target for RA.
format Online
Article
Text
id pubmed-9885117
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-98851172023-01-31 Increased expression of long-isoform thymic stromal lymphopoietin is associated with rheumatoid arthritis and fosters inflammatory responses Li, Wanlin Liao, Chenghui Du, Jing Hu, Jing Wang, Lu Song, Xun He, Zhendan Xiao, Xiaohua Ye, Liang Front Immunol Immunology Thymic stromal lymphopoietin (TSLP) is a pleiotropic cytokine that is involved in the pathogenesis of inflammatory diseases and asthma, but the expression and biological implications of the existence of two isoforms, long TSLP (lTSLP) and short TSLP (sTSLP), in RA have yet to be elucidated. Here we report that lTSLP is the predominant TSLP isoform in RA and active RA, whereas sTSLP is the major TSLP isoform in inactive RA and healthy controls. lTSLP expression is associated with disease activity, including 28-joint Disease Activity Score (DAS28) and erythrocyte sedimentation rate (ESR), as well as proinflammatory cytokine expression, irrespective of other laboratory parameters. Importantly, lTSLP alone or combined with LPS promotes the expression of proinflammatory cytokines IL-1β, IL-6, and IL-8 in PBMCs of RA, but restrains anti-inflammatory cytokine IL-10 expression in PBMCs of RA. Furthermore, we found that STAT5 signaling is involved in lTSLP-induced inflammatory accumulation in PBMCs of RA. Therefore, these results highlight the clinical significance of lTSLP in RA pathology and inflammatory response in acute-phase disease, which may provide a therapeutic target for RA. Frontiers Media S.A. 2023-01-16 /pmc/articles/PMC9885117/ /pubmed/36726974 http://dx.doi.org/10.3389/fimmu.2022.1079415 Text en Copyright © 2023 Li, Liao, Du, Hu, Wang, Song, He, Xiao and Ye https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Li, Wanlin
Liao, Chenghui
Du, Jing
Hu, Jing
Wang, Lu
Song, Xun
He, Zhendan
Xiao, Xiaohua
Ye, Liang
Increased expression of long-isoform thymic stromal lymphopoietin is associated with rheumatoid arthritis and fosters inflammatory responses
title Increased expression of long-isoform thymic stromal lymphopoietin is associated with rheumatoid arthritis and fosters inflammatory responses
title_full Increased expression of long-isoform thymic stromal lymphopoietin is associated with rheumatoid arthritis and fosters inflammatory responses
title_fullStr Increased expression of long-isoform thymic stromal lymphopoietin is associated with rheumatoid arthritis and fosters inflammatory responses
title_full_unstemmed Increased expression of long-isoform thymic stromal lymphopoietin is associated with rheumatoid arthritis and fosters inflammatory responses
title_short Increased expression of long-isoform thymic stromal lymphopoietin is associated with rheumatoid arthritis and fosters inflammatory responses
title_sort increased expression of long-isoform thymic stromal lymphopoietin is associated with rheumatoid arthritis and fosters inflammatory responses
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9885117/
https://www.ncbi.nlm.nih.gov/pubmed/36726974
http://dx.doi.org/10.3389/fimmu.2022.1079415
work_keys_str_mv AT liwanlin increasedexpressionoflongisoformthymicstromallymphopoietinisassociatedwithrheumatoidarthritisandfostersinflammatoryresponses
AT liaochenghui increasedexpressionoflongisoformthymicstromallymphopoietinisassociatedwithrheumatoidarthritisandfostersinflammatoryresponses
AT dujing increasedexpressionoflongisoformthymicstromallymphopoietinisassociatedwithrheumatoidarthritisandfostersinflammatoryresponses
AT hujing increasedexpressionoflongisoformthymicstromallymphopoietinisassociatedwithrheumatoidarthritisandfostersinflammatoryresponses
AT wanglu increasedexpressionoflongisoformthymicstromallymphopoietinisassociatedwithrheumatoidarthritisandfostersinflammatoryresponses
AT songxun increasedexpressionoflongisoformthymicstromallymphopoietinisassociatedwithrheumatoidarthritisandfostersinflammatoryresponses
AT hezhendan increasedexpressionoflongisoformthymicstromallymphopoietinisassociatedwithrheumatoidarthritisandfostersinflammatoryresponses
AT xiaoxiaohua increasedexpressionoflongisoformthymicstromallymphopoietinisassociatedwithrheumatoidarthritisandfostersinflammatoryresponses
AT yeliang increasedexpressionoflongisoformthymicstromallymphopoietinisassociatedwithrheumatoidarthritisandfostersinflammatoryresponses